| 芦希艳,李 琦,赵卫锋,董春燕,安 琦.参苓白术散联合针刺对溃疡性结肠炎大鼠内质网应激、炎症反应的作用机制[J].,2023,(11):2025-2029 |
| 参苓白术散联合针刺对溃疡性结肠炎大鼠内质网应激、炎症反应的作用机制 |
| Mechanism of ShenlingBaizhu Powder Combined with Acupuncture on Endoplasmic Reticulum Stress and Inflammation in Ulcerative Colitis Rats |
| 投稿时间：2022-11-11 修订日期：2022-12-06 |
| DOI：10.13241/j.cnki.pmb.2023.11.005 |
| 中文关键词: 参苓白术散 针刺 溃疡性结肠炎 内质网应激 炎症反应 |
| 英文关键词: Shenling Baizhu Powder Acupuncture Ulcerative colitis Endoplasmic reticulum stress The inflammatory response |
| 基金项目:2021年陕西省教育厅重点科研计划项目（21JS011） |
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| 摘要 目的：探讨参苓白术散联合针刺对溃疡性结肠炎大鼠内质网应激、炎症反应的作用机制。方法：采用三硝基苯磺酸的方法构建溃疡性结肠炎大鼠模型。给予大鼠参苓白术散联合针刺干预。采用ELISA法检测大鼠病变结肠的炎症反应和氧化应激水平；采用肉眼观察各组结肠组织形态学评分；采用Western blot 检测大鼠内质网应激相关蛋白的表达。结果：与空白组相比，模型组、针刺组、参苓白术散、参苓白术散组联合针刺组大鼠体质量更低（P＜0.05）；与模型组相比，针刺组、参苓白术散、参苓白术散组联合针刺组溃疡指数评分和大鼠体质量更低（P＜0.05），且参苓白术散组联合针刺组明显低于针刺组和参苓白术散组；与空白组相比，模型组、针刺组、参苓白术散、参苓白术散组联合针刺组ROS、GSH-Px、MDA更高（P＜0.05）；与模型组相比，针刺组、参苓白术散、参苓白术散组联合针刺组ROS、GSH-Px、MDA更低（P＜0.05），且参苓白术散组联合针刺组明显低于针刺组和参苓白术散组；与空白组相比，模型组、针刺组、参苓白术散、参苓白术散组联合针刺组TNF-α、IL-1β、IL-18更高（P＜0.05）；与模型组相比，针刺组、参苓白术散、参苓白术散组联合针刺组TNF-α、IL-1β、IL-18更低（P＜0.05），且参苓白术散组联合针刺组低于针刺组和参苓白术散组；与空白组相比，模型组、针刺组、参苓白术散、参苓白术散组联合针刺组GRP78、p-PERK、p-eIF2α更高（P＜0.05）；与模型组相比，针刺组、参苓白术散、参苓白术散组联合针刺组GRP78、p-PERK、p-eIF2α更低（P＜0.05），且参苓白术散组联合针刺组明显低于针刺组和参苓白术散组。结论：参苓白术散组联合针刺可有效抑制溃疡性结肠炎大鼠内质网应激，进而抑制炎症反应和氧化应激反应，并促进溃疡性结肠炎大鼠病变病情转归。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the mechanism of ShenlingBaizhu Powder combined with acupuncture on endoplasmic reticulum stress and inflammation in ulcerative colitis rats. Methods: The rat model of ulcerative colitis was established by tritrobenzene sulfonic acid method. The rats were treated with Shenling Baizhu Powder combined with acupuncture. The levels of inflammation and oxidative stress in the diseased colon of rats were detected by ELISA. The colonic histomorphological scores of each group were observed by naked eye. Western blot was used to detect the expression of endoplasmic reticulum stress related proteins. Results: Compared with blank group, the body weight of rats in model group, acupuncture group, Shenling Baizhu SAN group and Shenling Baizhu SAN combined acupuncture group were lower (P<0.05). Compared with model group, the ulcer index score and body weight of rats in acupuncture group, Shenling Baizhu SAN group and Shenling Baizhu SAN combined with acupuncture group were lower(P<0.05), and Shenling Baizhu SAN combined with acupuncture group was lower than acupuncture group and Shenling Baizhu SAN group (P<0.05). Compared with blank group, ROS, GSH-Px and MDA in model group, acupuncture group, Shenling Baizhu SAN group and Shenling Baizhu SAN combined with acupuncture group were higher(P<0.05). Compared with model group, ROS, GSH-Px and MDA of acupuncture group, Shenling Baizhu SAN group and Shenling Baizhu SAN combined with acupuncture group were lower(P<0.05), and Shenling Baizhu SAN combined with acupuncture group was lower than acupuncture group and Shenling Baizhu SAN group. Compared with blank group, TNF-α, IL-1β and IL-18 in model group, acupuncture group, ShenlingBaizhu SAN group and Shenling Baizhu SAN combined with acupuncture group were higher(P<0.05). Compared with model group, TNF-α, IL-1β and IL-18 of acupuncture group, Shenling Baizhu SAN group and Shenling Baizhu SAN combined with acupuncture group were lower(P<0.05), and Shenling Baizhu SAN combined with acupuncture group was lower than acupuncture group and Shenling Baizhu SAN group. Compared with blank group, GRP78, P-PERK and P-EIF2α in model group, acupuncture group, ShenlingBaizhu SAN group and Shenling Baizhu SAN combined acupuncture group were higher(P<0.05). Compared with model group, GRP78, P-PERK and P-EIF2α were lower in acupuncture group, Shenling Baizhu SAN group and Shenling Baizhu SAN combined with acupuncture group(P<0.05), and Shenling Baizhu SAN combined with acupuncture group was lower than acupuncture group and Shenling Baizhu SAN group. Conclusion: Shenling Baizhu Powder group combined with acupuncture can effectively inhibit endoplasmic reticulum stress in ulcerative colitis rats, and then inhibit inflammation and oxidative stress, and promote the prognosis of ulcerative colitis rats. |
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