| 曲 妍,张雪楠,张天予,陈 茜,王立新.2型糖尿病伴慢性牙周炎患者龈沟液Omentin-1、MMP-9、OPG/RANKL比值与牙周指标、氧化应激和NLRP3炎症小体的关系[J].,2023,(7):1257-1262 |
| 2型糖尿病伴慢性牙周炎患者龈沟液Omentin-1、MMP-9、OPG/RANKL比值与牙周指标、氧化应激和NLRP3炎症小体的关系 |
| Relationship between Gingival Crevicular Fluid Omentin-1, MMP-9, OPG/RANKL Ratio and Periodontal Indicators, Oxidative Stress and NLRP3 Inflammatorome in Type 2 Diabetes Mellitus with Chronic Periodontitis |
| 投稿时间：2022-11-05 修订日期：2022-11-28 |
| DOI：10.13241/j.cnki.pmb.2023.07.011 |
| 中文关键词: 2型糖尿病 慢性牙周炎 Omentin-1 MMP-9 OPG/RANKL比值 牙周指标 氧化应激 NLRP3炎症小体 相关性 |
| 英文关键词: Type 2 diabetes mellitus Chronic periodontitis Omentin-1 MMP-9 OPG/RANKL ratio Periodontal indicators Oxidative stress NLRP3 inflammatorome Correlation |
| 基金项目:北京市自然科学基金项目（7182175）；首都医科大学附属北京康复医院2020-2022年科技发展专项项目（2020-084） |
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| 中文摘要: |
| 摘要 目的：探讨2型糖尿病（T2DM）伴慢性牙周炎（CP）患者龈沟液网膜素-1（Omentin-1）、基质金属蛋白酶-9（MMP-9）、骨保护素（OPG）/细胞核因子κB受体活化因子配体（RANKL）比值与牙周指标、氧化应激和核苷酸结合寡聚化结构域样受体蛋白3（NLRP3）炎症小体的关系。方法：选择2020年6月至2022年6月首都医科大学附属北京康复医院收治的73例T2DM患者（T2DM组），77例CP患者（CP组），83例T2DM伴CP患者（T2DM伴CP组）。检测所有患者龈沟液中Omentin-1、MMP-9、OPG/RANKL比值，分析其与牙周指标、氧化应激和NLRP3炎症小体相关分子信使核糖核酸（mRNA）表达的相关性。结果：T2DM伴CP组龈沟液中Omentin-1，OPG/RANKL比值、总抗氧化能力（TAC）、超氧化物歧化酶（SOD）低于T2DM组和CP组（P＜0.05），MMP-9、丙二醛（MDA）、NLRP3mRNA、凋亡相关斑点样蛋白（ASC）mRNA、半胱氨酸蛋白酶-1（caspase-1）mRNA表达以及出血指数（SBI）、菌斑指数（PLI）、牙周袋探诊深度（PD）、附着丧失（AL）高于T2DM组和CP组（P＜0.05）。T2DM伴CP患者龈沟液中Omentin-1、OPG/RANKL比值与TAC、SOD呈正相关（P＜0.05），与MDA、NLRP3mRNA、ASC mRNA、caspase-1mRNA表达以及PLI、SBI、AL、PD呈负相关（P＜0.05），MMP-9与TAC、SOD呈负相关（P＜0.05），与MDA、NLRP3mRNA、ASC mRNA、caspase-1mRNA表达以及PLI、SBI、AL、PD呈正相关（P＜0.05）。结论：T2DM伴CP患者龈沟液中Omentin-1水平、OPG/ RANKL比值降低，MMP-9水平升高，与牙周组织破坏加重、氧化应激、NLRP3炎症小体激活有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the relationship between the gingival crevicular fluid Omentin-1, matrix metalloproteinase-9 (MMP-9), osteopterin (OPG)/receptor activator of nuclear factor-κBligand (RANKL) ratio and periodontal indicators, oxidative stress and nucleotide binding oligomerization domain-like receptor protein 3 (NLRP3) inflammasome in type 2 diabetes mellitus (T2DM) with chronic periodontitis (CP). Methods: 73 patients with T2DM (T2DM group), 77 patients with CP (CP group), and 83 patients with T2DM with CP (T2DM with CP group) who were admitted to Beijing Rehabilitation Hospital affiliated to Capital Medical University from June 2020 to June 2022 were selected. The Omentin-1, MMP-9 and OPG/RANKL ratio in gingival creval fluid of all patients were detected, and their correlations with periodontal indicators, oxidative stress and NLRP3 inflammator-related molecule messenger RNA (mRNA) expression were analyzed. Results: The gingival crevicular fluid Omentin-1, OPG/RANKL ratio, total antioxidant capacity (TAC) and superoxide dismutase (SOD) in the T2DM with CP group were lower than those in the T2DM group and CP group (P＜0.05). The MMP-9, malonaldehyde (MDA), NLRP3mRNA, apoptosis-related speck-like protein (ASC) mRNA, cysteine protease-1 (caspase-1) mRNA expressions, and bleeding index (SBI), plaque index (PLI), periodontal pocket probing depth (PD) and attachment loss (AL) were higher than those in the T2DM group and CP group(P＜0.05). The gingival crevicular fluid Omentin-1 and OPG/RANKL ratio in patients with T2DM with CP were positively correlated with TAC and SOD (P＜0.05), they were negatively correlated with the MDA, NLRP3mRNA, ASC mRNA, caspase-1mRNA expressions, and PLI, SBI, AL, PD (P＜0.05), and MMP-9 was negatively correlated with TAC and SOD (P＜0.05), it was positively correlated with the MDA, NLRP3mRNA, ASC mRNA, Caspase-1 mRNA expressions, and PLI, SBI, AL, PD (P＜0.05). Conclusion: The gingival crevicular fluid Omentin-1 level, OPG/ RANKL ratio in patients with T2DM with CP are decreased, and MMP-9 level are increased, which are associated with aggravated periodontal tissue destruction, oxidative stress and activation of NLRP3 inflammatorome. |
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