| 吴雪芳,瞿 菲,刘 谦,卢蓉蓉,黄 香,孙春晓,付子毅,殷咏梅,李 薇.LNCRNA FAR2P1对乳腺癌细胞增殖迁移和凋亡的影响研究[J].,2023,(5):801-806 |
| LNCRNA FAR2P1对乳腺癌细胞增殖迁移和凋亡的影响研究 |
| Effect of LNCRNA FAR2P1 on Proliferation, Migration and Apoptosis of Breast Cancer Cells |
| 投稿时间：2022-09-25 修订日期：2022-10-21 |
| DOI：10.13241/j.cnki.pmb.2023.05.001 |
| 中文关键词: 长链非编码 RNA FAR2P1 乳腺癌 增殖 迁移 |
| 英文关键词: Long non-coding RNA FAR2P1 Breast cancer Proliferation Migration |
| 基金项目:国家自然科学基金面上项目（81772475）；江苏省自然科学基金面上项目（BK20191355） |
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| 中文摘要: |
| 摘要 目的：探讨新型 LncRNA FAR2P1在乳腺癌发生发展中的作用和影响。方法：癌症基因组图谱（The cancer genome atlas, TCGA, https://portal.gdc.cancer.gov/）数据库分析LncRNA FAR2P1差异表达量。根据169例LncRNA FAR2P1高表达的乳腺癌患者和1180例LncRNA FAR2P1 低表达患者随访资料，分析LncRNA FAR2P1与乳腺癌患者预后的相关性。首先利用实时定量荧光聚合酶链反应(qRT-PCR)检测LncRNA FAR2P1在乳腺癌细胞系（MDA-MB-231、SKBR3）和乳腺正常上皮细胞（MCF-10A）中的表达水平；转染小干扰（si-RNA）获得低表达 FAR2P1的细胞株, Cell Counting Kit-8（CCK8）实验、EDU实验、克隆形成评估细胞增殖活力，Transwell 实验探究 FAR2P1 对细胞迁移的影响；通过流式分析FAR2P1 是否调节细胞凋亡；体内异种成瘤模型评估 FAR2P1 在体内对乳腺癌增值的调节作用。结果：LncRNA FAR2P1在MDA-MB-231、SKBR3中表达显著升高，并且与乳腺癌患者预后负相关。体外实验表明，敲低MDA-MB-231和SKBR3细胞中的 FAR2P1 后，细胞的增殖能力和迁移能力减弱，但是凋亡率明显升高。体内异种成瘤模型显示沉默 FAR2P1相比对照，乳腺癌细胞增殖能力显著降低。结论：LncRNA FAR2P1 在乳腺癌中高表达，并且参与调控乳腺癌增殖、凋亡和迁移。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the biological role and influence of novel LncRNA FAR2P1 in the tumorigenesis and progress of breast cancer. Methods: The cancer genome atlas（TCGA, https://portal.gdc.cancer.gov/) database was used to analyze the differential expression of LncRNA FAR2P1. According to the follow-up data of 169 breast cancer patients with high expression of LncRNA FAR2P1 and 1180 breast cancer patients with low expression of LncRNA FAR2P1, the relation between LncRNA FAR2P1 and the prognosis was analyzed. Qrt-pcr was used to explore the expression of LncRNA FAR2P1 in McF-10a and MDA-MB-231 SKBR3 cells. The effects of FAR2P1 on the proliferation and migration of cells were evaluated by in vitro experiments, such as Cell Counting KIT-8 (CCK8) method, EDU method, clone formation method and Transwell method.Flow cytometry was used to detect whether FAR2P1 regulates the apoptosis process of cells. The regulatory effect of FAR2P1 on cell proliferation in vivo was further investigated. Results: The expression of LncRNA FAR2P1 is obviously increased in breast cancer tissues and cell line MDA-MB-231 SKBR3, and is negatively relate with the prognosis of patients. Compared with the control, the silencing FAR2P1 significantly reduced the proliferation and migration ability of MDA-MB-231 and SKBR3, but improved the proportion of apoptosis.In vivo xenogenic tumor model showed that silencing FAR2P1 markedly decreased the proliferation ability of breast cancer compared with the control groups. Conclusion: LncRNA FAR2P1 is highly expressed in breast cancer and plays an important role in regulating the proliferation, apoptosis and migration of breast cancer. |
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