| 叶良静,李 慧,孙卫国,陈 艳,徐小倩.龈沟液miR-155、miR-223表达水平与慢性牙周炎伴2型糖尿病患者牙周临床指标、口腔龈下菌群以及Th17/Treg失衡的相关性分析[J].,2023,(2):350-355 |
| 龈沟液miR-155、miR-223表达水平与慢性牙周炎伴2型糖尿病患者牙周临床指标、口腔龈下菌群以及Th17/Treg失衡的相关性分析 |
| Correlation Analysis of the Expression Levels of miR-155 and miR-223 in Gingival Crevicular Fluid and Periodontal Clinical Indicators, Oral Subgingival Microbiota and Th17/Treg Imbalancein Patients with Chronic Periodontitis with Type 2 Diabetes Mellitus |
| 投稿时间：2022-05-21 修订日期：2022-06-17 |
| DOI：10.13241/j.cnki.pmb.2023.02.028 |
| 中文关键词: 慢性牙周炎 2型糖尿病 口腔菌群 牙周临床指标 辅助性T细胞17 调节性T细胞 相关性 |
| 英文关键词: Chronic periodontitis Type 2 diabetes Oral flora Periodontal clinical indicators T helper cell 17 Regulatory T cells Correlation |
| 基金项目:安徽省卫生计生委科研计划项目（2016qk1208） |
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| 中文摘要: |
| 摘要 目的：探讨龈沟液miR-155、miR-223表达水平与慢性牙周炎伴2型糖尿病（T2DM）患者牙周临床指标、口腔龈下菌群以及外周血辅助性T细胞17（Th17）/调节性T细胞（Treg）失衡的相关性。方法：选择2018年1月至2022年1月安徽理工大学第一附属医院口腔科收治的86例慢性牙周炎患者，根据是否伴T2DM将患者分为慢性牙周炎伴T2DM组15例和单纯慢性牙周炎组71例，另选择65例健康体检志愿者为对照组。检测龈沟液miR-155、miR-223表达水平，口腔龈下菌群以及外周血Th17细胞占比、Treg细胞占比、血清白细胞介素（IL）-17、转化生长因子-β（TGF-β）水平。Pearson相关分析龈沟液miR-155、miR-223表达水平与牙周临床指标、口腔龈下菌群、外周血Th17/Treg以及血清IL-17、TGF-β的相关性。结果：慢性牙周炎伴T2DM组、单纯慢性牙周炎组龈沟液miR-155、miR-223表达水平高于对照组（P＜0.05），且慢性牙周炎伴T2DM组高于单纯慢性牙周炎组（P＜0.05）。慢性牙周炎伴T2DM组龈沟出血指数（SBI）、菌斑指数（PLI）、探诊深度（PD）、附着丧失（AL）、牙龈卟啉单胞菌、二氧化碳噬纤维菌、中间普氏菌、变黑普氏菌数量、外周血Th17细胞占比、Th17/Treg比值、血清IL-17水平高于单纯慢性牙周炎组（P＜0.05），外周血Treg细胞占比低于单纯慢性牙周炎组（P＜0.05）。龈沟液miR-155、miR-223表达水平与PLI、SBI、AL、PD、牙龈卟啉单胞菌、二氧化碳噬纤维菌、中间普氏菌、变黑普氏菌数量、外周血Th17细胞占比、Th17/Treg比值、血清IL-17水平呈正相关（P＜0.05），与外周血Treg细胞占比呈负相关（P＜0.05）。结论：慢性牙周炎伴T2DM患者龈沟液中miR-155、miR-233表达均上调，且与牙周组织破坏程度、龈下菌群紊乱和Th17/Treg失衡有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the correlation between the expression levels of miR-155 and miR-223 in gingival crevicular fluid and periodontal clinical indicators, oral subgingival microbiota and peripheral blood helper T cell 17 (Th17)/regulatory T cell (Treg) imbalance in patients with chronic periodontitis with type 2 diabetes mellitus (T2DM). Methods: 86 patients with chronic periodontitis who were admitted to the Department of Stomatology, the First Affiliated Hospital of Anhui University of Science and Technology from January 2018 to January 2022 were selected, and they were divided into chronic periodontitis with T2DM group with 15 cases and simple chronic periodontitis group with 71 cases according to whether they had T2DM. In addition, 65 healthy volunteers were selected as the control group. The expression levels of miR-155 and miR-223 in gingival crevicular fluid, oral subgingival microbiota, the proportion of peripheral blood Th17 cells and proportion of Treg cells, serum interleukin (IL)-17 and transforming growth factor-β (TGF-β) were detected. Pearson correlation analysis was performed to analyze the correlation between the expression levels of miR-155 and miR-223 in gingival crevicular fluid and periodontal clinical indicators, oral subgingival microbiota, peripheral blood Th17/Treg, serum IL-17 and TGF-β. Results: The expression levels of miR-155 and miR-223 in gingival crevicular fluid in chronic periodontitis with T2DM group and simple chronic periodontitis group were higher than those in control group (P<0.05), and the chronic periodontitis with T2DM group was higher than the simple chronic periodontitis group (P<0.05). The gingival crevicular bleeding index (SBI), plaque index (PLI), probing depth (PD), attachment loss (AL), Porphyromonas gingivalis, Carbon dioxide-eating fibroblasts, Prevotella intermedia, Prevotella nigrescens, the proportion of peripheral blood Th17 cells, Th17/Treg ratio, and serum IL-17 level in chronic periodontitis with T2DM group were higher than those in simple chronic periodontitis group (P<0.05), the proportion of peripheral blood Treg cells was lower than that in simple chronic periodontitis group (P<0.05). The expression levels of miR-155 and miR-223 in gingival crevicular fluid were positively correlated with PLI, SBI, AL, PD, Porphyromonas gingivalis, carbon dioxide-eating fibroblasts, Prevotelia intermedia, Prevotelia melanogra, the proportion of peripheral blood Th17 cells, Th17/Treg ratio, and serum IL-17 level (P<0.05), they were negatively correlated with the proportion of peripheral blood Treg cells (P<0.05). Conclusion: The expressions of miR-155 and miR-233 in gingival crevicular fluid of patients with chronic periodontitis witn T2DM are up-regulated, which are related to the degree of periodontal tissue destruction, subgingival microbiota disorder and Th17/Treg imbalance. |
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