| 张银福,白 静,高 巍,王 乐,庞 芸,陈颖力.芬太尼联合电针通过介导HDAC2途径调节糖尿病大鼠周围神经痛的作用机制研究[J].,2022,(23):4430-4434 |
| 芬太尼联合电针通过介导HDAC2途径调节糖尿病大鼠周围神经痛的作用机制研究 |
| Mechanism of Fentanyl Combined with Electroacupuncture in Regulating Peripheral Neuralgia in Diabetic Rats by Mediating HDAC2 Pathway |
| 投稿时间：2022-03-27 修订日期：2022-04-23 |
| DOI：10.13241/j.cnki.pmb.2022.23.006 |
| 中文关键词: 芬太尼 电针 糖尿病周围神经痛 机械痛阈 体重 胰岛素 组蛋白去乙酰化酶2 |
| 英文关键词: Fentanyl Electroacupuncture Diabetic peripheral neuralgia Mechanical pain threshold Body weight Insulin Histone deacetylase 2 |
| 基金项目:国家自然科学基金面上项目（81971290） |
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| 中文摘要: |
| 摘要 目的：探讨与研究芬太尼联合电针通过介导组蛋白去乙酰化酶2（histone deacetylase 2，HDAC2）途径调节糖尿病大鼠周围神经痛的作用机制。方法：将建模成功的糖尿病周围神经痛大鼠（n=36）随机平分为三组-模型组、芬太尼组与电针组，芬太尼组、模型组分别经尾静脉泵注1.0 μg/kg/min芬太尼与等剂量的磷酸盐缓冲液5 min，1次/d。电针组在芬太尼治疗的基础上给予电针治疗，1次/d，均共治疗2周。治疗第1周与第2周，对大鼠进行体重称重，采用双抗体酶联免疫夹心法测血清胰岛素浓度，动态足底触觉仪检测大鼠机械痛阈，免疫印迹检测HDAC2蛋白相对表达水平。结果：芬太尼组与电针组在治疗第1周、第2周的体重都明显高于模型组（P<0.05），电针组也明显高于芬太尼组（P<0.05）。芬太尼组与电针组在治疗第1周、第2周的血清胰岛素浓度都明显低于模型组（P<0.05），电针组也明显高于芬太尼组（P<0.05）。芬太尼组与电针组在治疗第1周、第2周的机械痛阈都明显高于模型组（P<0.05），电针组明显高于芬太尼组（P<0.05）。芬太尼组与电针组在治疗第2周、第4周的 HDAC2蛋白表达水平明显高于模型组（P<0.05），电针组也显著高于芬太尼组（P<0.05）。结论：芬太尼联合电针在糖尿病周围神经痛大鼠的应用能提高机械痛阈，能提高大鼠体重，也可降低胰岛素浓度，其作用机制可能与促进HDAC2表达有关。 |
| 英文摘要: |
| ABSTRACT Objective: To explore and study the mechanism of fentanyl combined with electroacupuncture in regulating peripheral neuralgia in diabetic rats by mediating histone deacetylase 2 (HDAC2) pathway. Methods: The successfully modeled diabetic peripheral neuralgia rats (n=36) were randomly divided into three groups - model group, fentanyl group and electroacupuncture group. The fentanyl group and model group were injected with 1.0 μg/kg/min fentanyl and equal dose of phosphate buffer for 5 min, once a day. The electroacupuncture group was given electroacupuncture on the basis of fentanyl treatment, once a day, for a total of 2 weeks. In the first week and the second week of treatment, the rats were weighed, and the serum insulin concentration was measured by double-antibody enzyme-linked immunosorbent assay, the mechanical pain threshold of rats was detected by dynamic plantar tactile instrument, and the relative expression level of HDAC2 protein was detected by western blotting. Results: The body weight of the fentanyl group and the electroacupuncture group were significantly higher than that of the model group in the first week and the second week of treatment (P<0.05), and the electroacupuncture group were also significantly higher than the fentanyl group (P<0.05). The serum insulin concentrations of the fentanyl group and the EA group were significantly lower than those of the model group in the first and second weeks of treatment (P<0.05), and the electroacupuncture group were also significantly higher than the fentanyl group (P<0.05). The mechanical pain thresholds of the fentanyl group and the electroacupuncture group were significantly higher than those of the model group at the first and second week of treatment (P<0.05), and the electroacupuncture group were significantly higher than the fentanyl group (P<0.05). The expression levels of HDAC2 protein in the fentanyl group and the EA group were significantly higher than those in the model group at the second and fourth week of treatment(P<0.05), and the EA group were also significantly higher than the fentanyl group (P<0.05). Conclusion: The application of fentanyl combined with electroacupuncture in rats with diabetic peripheral neuralgia can improve the mechanical pain threshold, increase the body weight of the rats, and reduce the concentration of insulin. The mechanism of action may be related to the promotion of HDAC2 expression. |
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