| 陶明源,高长玉,高 红,赵耀伟,韩东卫.SIRT7琥珀酰化修饰对肝癌生存、免疫浸润及预后的相关性分析[J].,2022,(21):4049-4054 |
| SIRT7琥珀酰化修饰对肝癌生存、免疫浸润及预后的相关性分析 |
| Correlation Analysis of Succinylation of SIRT7 in Survival, Immune Invasion and Prognosis of Hepatocellular Carcinoma |
| 投稿时间：2022-06-21 修订日期：2022-07-15 |
| DOI：10.13241/j.cnki.pmb.2022.21.008 |
| 中文关键词: 肝癌 琥珀酰化 SIRT7 糖酵解 生信分析 |
| 英文关键词: Cancer of the liver Succinylation SIRT7 Glycolysis Bioinformatics analysis |
| 基金项目:黑龙江省自然科学基金项目（YQ2021H026）；黑龙江中医药大学"优秀创新人才支持计划"科研项目；中国博士后科学基金资助项目（2020M670942） |
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| 中文摘要: |
| 摘要 目的：探究SIRT7基因琥珀酰化修饰对肝癌患者的生存、免疫浸润及预后的相关性分析。方法：采用生物信息分析法对SIRT7在肝癌组织中的表达情况及其对肝癌患者预后的影响进行分析；采用蛋白免疫印迹法（Western blot）检测其转染效果。结果：（1）生物信息分析结果显示：SIRT7在多种肿瘤（包括肝癌）组织中呈高表达（P<0.05）；SIRT7的表达与肿瘤的生存曲线相关（P<0.05）；肝癌患者的SIRT7相对表达量与其预后相关，高表达组肝癌患者的总生存情况（P=0.017）和无进展生存情况较低表达组缩短（P=0.004）；免疫浸润和肿瘤微环境分析结果显示，SIRT7表达水平与多数免疫细胞浸润水平、肿瘤微环境(ESTIMATES core)均有明显负相关。（2）Western blot显示，SIRT7在肝癌细胞中表达高于正常细胞。因此，SIRT7 可作为肝癌的潜在预后标志物。结论：SIRT7表达水平与肝癌(HCC)患者的预后、免疫细胞浸润性、肿瘤微环境免疫细胞和基质细胞浸润等相关。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the correlation analysis of succinylation for SIRT7 gene on survival, immune invasion and prognosis of hepatocellular carcinoma. Methods: The expression of SIRT7 in HCC and its effect on prognosis of HCC patients were analyzed by bioinformatics analysis. The transfection effect was detected by Western blot. Real-time fluorescence quantitative polymerase short reaction (qRT-PCR) was used to detect the changes of epithelial-mesenchymal transformation (EMT) related genes after silencing SIRT7. Results: (1) Bioinformatics analysis showed that SIRT7 was highly expressed in various tumor tissues (including liver cancer) (P<0.05). The expression of SIRT7 was correlated with tumor survival curve (P<0.05). The relative expression level of SIRT7 in HCC patients was correlated with its prognosis. The overall survival (P=0.017) and progression-free survival (P=0.004) of HCC patients in the high expression group were shorter than those in the low expression group. The results of immune invasion and tumor microenvironment analysis showed that the expression level of SIRT7 was significantly correlated with the levels of most immune cell invasion and tumor microenvironment (ESTIMATEScore), and both were negatively correlated. (2) Western blotting showed that SIRT7 expression was higher in HCC cells than in normal cells. Therefore, SIRT7 can be used as a potential prognostic marker of liver cancer. Conclusion: The expression level of SIRT7 is correlated with the prognosis of HCC patients, the infiltration of immune cells, and the infiltration of immune cells and stromal cells in tumor microenvironment. |
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