| 杨晴晴,曹婉悦,赵秋燕,贾雪冰,陈立晓.MiR-1-3p通过抑制CAPRIN1调控胰腺癌发生发展[J].,2022,(18):3401-3407 |
| MiR-1-3p通过抑制CAPRIN1调控胰腺癌发生发展 |
| MiR-1-3p Regulates the Development of Pancreatic Cancer via Inhibiting CAPRIN1 |
| 投稿时间：2022-03-12 修订日期：2022-04-07 |
| DOI：10.13241/j.cnki.pmb.2022.18.001 |
| 中文关键词: miR-1-3P CAPRIN1 细胞周期 胰腺癌 |
| 英文关键词: MiR-1-3p CAPRIN1 Cell cycle Pancreatic cancer |
| 基金项目:国家自然科学基金项目（81800893） |
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| 中文摘要: |
| 摘要 目的：探讨miR-1-3p在胰腺癌发生发展中的分子机制。方法：以MIA-PaCa-2，SW 1990为研究目标，通过qRT-PCR技术检测miR-1-3p的表达量，利用TargetScan和miRDB数据库预测miR-1-3p的下游靶基因及结合位点，并通过构建双荧光素酶报告基因，进一步确认miR-1-3p与靶基因的结合。利用CCK8细胞增殖实验及平板克隆形成实验检测过表达miR-1-3p及敲低CAPRIN1对细胞增殖的作用；利用流式检测细胞周期；利用蛋白质免疫印迹方法检测miR-1-3p对CAPRIN1及其下游基因的影响；通过流式来确认，过表达miR-1-3p及敲减CAPRIN1基因对细胞周期的影响。结果：miR-1-3p在胰腺癌细胞MIA-PaCa-2，SW 1990中低表达；miR-1-3p直接与CAPRIN1的3'-untranslated region (3'- UTR)结合；过表达miR-1-3p或抑制CAPRIN1基因的表达可明显抑制胰腺癌细胞的增殖能力，同时也产生细胞周期阻滞。结论：miR-1-3p通过抑制CAPRIN1基因表达，而产生细胞周期阻滞进而抑制胰腺癌细胞的增殖能力。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the molecular mechanism of mir-1-3p in the development and progression of pancreatic cancer. Methods: With MIA-PACA-2 and SW 1990 as the research target, the expression level of mir-1-3p was detected by qRT-PCR technology, and the downstream target genes and binding sites of mir-1-3p were predicted by TargetScan and miRDB databases, and the double luciflucase reporter gene was constructed, the binding of miR-1-3p to target genes was further confirmed. CCK8 cell proliferation assay and plate clonal formation assay were used to detect the effects of overexpression of miR-1-3p and knockdown CAPRIN1 on cell proliferation. Western blot was used to detect the effect of miR-1-3p on CAPRIN1 and its downstream genes. Finally, the effects of miR-1-3p overexpression and CAPRIN1 knockdown on cell cycle were confirmed by flow cytometry. Results: In pancreatic cancer cells MIA-PACA-2, SW 1990, miR-1-3p was low in mRNA level. Mir-1-3p directly binds to the 3'-untranslated region (3' -UTR) of CAPRIN1. Overexpression of miR-1-3p or inhibition of CAPRIN1 gene expression can significantly inhibit the proliferation of pancreatic cancer cells, and also induce cell cycle arrest. Conclusion: MiR-1-3p inhibits the proliferation of pancreatic cancer cells by inhibiting the expression of CAPRIN1 gene, resulting in cell cycle arrest. |
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