| 刘晓卉,潘姝杉,魏小倩,苗茸茸,徐平湘.植物乳杆菌P-8对小鼠免疫功能的调节作用研究[J].,2022,(16):3005-3009 |
| 植物乳杆菌P-8对小鼠免疫功能的调节作用研究 |
| Immunomodulatory Effect of Lactobacillus Plantarum P-8 in Mice |
| 投稿时间：2021-10-31 修订日期：2021-11-26 |
| DOI：10.13241/j.cnki.pmb.2022.16.002 |
| 中文关键词: 植物乳杆菌P-8 小鼠 免疫功能 剂量 |
| 英文关键词: Lactobacillus plantarum P-8 Mouse Immunomodulatory effect Dose |
| 基金项目:北京市自然科学基金面上项目（7172029） |
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| 中文摘要: |
| 摘要 目的：不同类型的益生菌株免疫调节功能各异。本文旨在评价植物乳杆菌P-8（Lactobacillus plantarum P-8）对小鼠免疫功能的调控作用及机制。方法：C57BL/6J小鼠每日灌胃给予不同剂量的植物乳杆菌P-8（0. 25 mg/kg、0.5 mg/kg、1.5 mg/kg），连续30天，记录小鼠一般情况。给药结束后处死动物，测定小鼠脏器/体重比；小鼠碳廓清实验、小鼠腹腔巨噬细胞吞噬鸡红细胞实验评价各组小鼠的单核-巨噬细胞功能；血清溶血素测定、抗体生成细胞实验评价各组小鼠的体液免疫功能；脾淋巴细胞转化实验、迟发型变态反应实验评价各组小鼠的细胞免疫功能；NK细胞的活性测定实验评价小鼠的NK细胞活性。结果：与对照组相比，低、中、高剂量组植物乳杆菌P-8对小鼠脏器/体重比值差异无统计学意义（P>0.05）；且植物乳杆菌P-8可显著提高小鼠的碳廓清能力、小鼠腹腔巨噬细胞吞噬鸡红细胞能力、半数溶血值、二硝基氟苯诱导的小鼠迟发型变态反应及NK细胞活力（P均<0.05）。结论：植物乳杆菌P-8可通过提高单核-巨噬细胞功能、体液免疫功能、细胞免疫功能及NK细胞活力增强小鼠的免疫功能。 |
| 英文摘要: |
| ABSTRACT Objective: Different types of probiotics have different immunomodulatory functions. The present study aimed to evaluate the immunomodulatory effects of Lactobacillus plantarum P-8 in mice and its mechanism. Methods: Normal C57BL/6J mice received intragastric administration of Lactobacillus plantarum P-8 (0.25 mg/kg, 0.5 mg/kg, and 1.5 mg/kg) once daily for 30 days, while a control group received the same amount of water. The immunity of mice was evaluated by their organ ratios. The function of monocyte macrophage was evaluated by carbon clearance test and mouse peritoneal macrophage phagocytosis of chicken red blood cells. The humoral immunity of mice was evaluated by serum hemolysin assay and antibody producing cell assay. The cellular immunity was evaluated by ConA-induced mouse splenic lymphocyte transformation assay and delayed hypersensitivity induced by DNFB. NK cell activity was evaluated by NK cell activity assay. Results: Compared with the control group, Lactobacillus plantarum P-8 showed no significant effects on organ ratios of the mice. Lactobacillus plantarum P-8 significantly increased the murine macrophage carbon clearance function, mouse peritoneal macrophage phagocytosis of chicken red blood cells, hemolytic activity, delayed hypersensitivity induced by dinitrofluorobenzene, and NK cell activity. Conclusion: Lactobacillus plantarum P-8 exhibited potent immunomodulatory effects by increasing function of monocyte macrophage, cellular immunity, humoral immunity, and NK cell activity. |
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