| 时孝晴,揭立士,吴 鹏,茆 军,张农山,王培民,殷松江.SS-31通过调控线粒体功能延缓细胞衰老的分子机制[J].,2022,(10):1834-1840 |
| SS-31通过调控线粒体功能延缓细胞衰老的分子机制 |
| The Molecular Mechanism of SS-31 Mediating Mitochondrial Function to Delay the Senescence of HEK293T Cells |
| 投稿时间：2021-10-26 修订日期：2021-11-21 |
| DOI：10.13241/j.cnki.pmb.2022.10.008 |
| 中文关键词: SS-31 线粒体功能 细胞衰老 |
| 英文关键词: SS-31 Mitochondrial function Cell senescence |
| 基金项目:江苏省自然科学基金青年项目（SBK2020041404）；国家自然科学基金青年项目（82074460）；江苏省研究生科研与实践创新计划项目（KYCX21_1684）；江苏省高校自然科学基金面上项目（20KJB360003） |
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| 中文摘要: |
| 摘要 目的：探讨SS-31通过调控线粒体功能延缓细胞衰老的调控效应。方法：将HEK293T细胞分为空白组、模型组、SS-31组。使用H2O2诱导应激性衰老模型，然后采用SS-31对HEK293T细胞进行干预。SA-β-gal衰老染色试剂盒检测细胞衰老水平；JC-1试剂盒检测细胞线粒体膜电位水平；荧光倒置显微镜观察线粒体活性氧（ROS）荧光强度；ATP检测试剂盒检测细胞内ATP水平；蛋白质免疫印迹检测P53，P21，Acetyl-p53，Sirt1蛋白表达。结果：模型组SA-β-gal阳性率高于空白组，应用SS-31后阳性率显著降低，差异具有统计学意义（P＜0.05）；ROS荧光检测：与空白组比较，模型组荧光强度上升，而SS-31组荧光强度较模型组下降，差异有统计学意义（P＜0.05）；线粒体膜电位检测：与空白组比较，模型组红色荧光强度显著下降，SS-31干预后，红色荧光强度显著上升，差异有统计学意义（P＜0.05）；ATP检测：模型组ATP水平低于对照组，SS-31组高于模型组，差异有统计学意义（P＜0.05）；此外，模型组P53，P21及Acetyl-p53蛋白表达水平较空白组增加（P＜0.05），而SS-31组较模型组有所降低，差异有统计学意义（P＜0.05），模型组Sirt1蛋白表达水平较空白组降低（P＜0.05），而SS-31组较模型组升高，差异有统计学差异（P＜0.05）。结论：SS-31能够通过改善线粒体功能延缓HEK293T细胞衰老。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the regulatory effect of SS-31 in delaying cell senescence through mediating mitochondrial function. Methods: The HEK293T cell were divided into blank group, model group and SS-31 group. Use H2O2 to induce a stress-induced aging model, and then use SS-31 to intervene in HEK293T cell. SA-β-gal senescence staining kit detects cell senescence level; JC-1 kit detects cell mitochondrial membrane potential level; fluorescence inverted microscope observes mitochondrial reactive oxygen species (ROS) fluorescence intensity; ATP detection kit detects intracellular ATP level; protein Western blotting was used to detect the expression levels of P53, P21, Acetyl-p53 and Sirt1. Results: The percentage of SA-β-gal positive cells in the model group was higher than that of the blank group, and the percentage of SA-β-gal positive cells in the SS-31 group was reduced, and the difference was statistically significant (P＜0.05); ROS fluorescence detection found that the model group produced a lot of Green fluorescence, while the fluorescence intensity of the SS-31 group decreased, and the difference was statistically significant (P＜0.05); mitochondrial membrane potential detection showed that the red fluorescence intensity of the model group decreased significantly compared with the blank group. After the intervention of SS-31, the red fluorescence The intensity increased significantly, and the difference was statistically significant (P<0.05); ATP detection showed that the ATP level of the model group was lower than that of the control group, and the SS-31 group was higher than the model group, and the difference was statistically significant (P<0.05); The protein expression levels of P53, P21 and Acetyl-p53 increased compared with the blank group (P＜0.05), while the SS-31 group was lower than the model group. The difference was statistically significant (P＜0.05). The expression level of Sirt1 protein in the model group was higher than that of the blank group. The blank group decreased (P<0.05), while the SS-31 group was higher than the model group, the difference was statistically significant (P<0.05). Conclusion: SS-31 can improve the function of mitochondria, thereby delaying the senescence of HEK293T cell. |
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