| 曾志兰,周建寅,刘康华,朱立忠,庞 雁.桑叶黄酮调控糖尿病模型小鼠心肌线粒体功能和纤维化进展的机制[J].,2022,(7):1234-1238 |
| 桑叶黄酮调控糖尿病模型小鼠心肌线粒体功能和纤维化进展的机制 |
| Mechanism of Mulberry Leaf Flavonoids Regulating Myocardial Mitochondrial Function and Fibrosis Progression in Diabetic Model Mice |
| 投稿时间：2021-09-07 修订日期：2021-09-30 |
| DOI：10.13241/j.cnki.pmb.2022.07.008 |
| 中文关键词: 桑叶黄酮 糖尿病 线粒体 纤维化 |
| 英文关键词: Mulberry leaf flavonoids Diabetes Mitochondria Fibrosis |
| 基金项目:江苏省妇幼保健协会科研课题立项（FYX201808） |
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| 中文摘要: |
| 摘要 目的：研究桑叶黄酮对糖尿病小鼠心肌线粒体功能和心肌纤维化的影响，并探讨其作用的具体分子机制。方法：45只ICR小鼠，随机分为3组，即正常组、模型组和桑叶黄酮组。模型组和桑叶黄酮组通过腹腔注射四氧嘧啶生理盐水溶液建立糖尿病模型，正常组小鼠腹腔注射生理盐水。桑叶黄酮组糖尿病小鼠在模型成功建立后灌胃给予桑叶黄酮（1.0 g/kg/天）治疗，正常组和模型组给予等量生理盐水治疗。治疗6周后，测定并比较三组小鼠血糖、血胰岛素、肝糖原、肝己糖激酶（HK）和丙酮酸激酶（PK）含量，心肌线粒体谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、总钙和ATP含量，以及心脏CD31、α-SMA和Collagen I mRNA表达。结果：研究期间，模型组和桑叶黄酮组小鼠分别死亡3只和1只。经桑叶黄酮治疗6周后，糖尿病小鼠血糖显著降低，血胰岛素水平、肝糖原、肝HK和肝PK含量，心肌线粒体GSH、SOD、总钙和ATP含量均显著增高（P<0.05）。心肌纤维化指标：糖尿病小鼠心肌CD31 mRNA表达水平经桑叶黄酮治疗后显著增高，而α-SMA和Collagen I mRNA表达水平却显著降低（P<0.05）。结论：桑叶黄酮可显著降低糖尿病小鼠血糖水平，改善其糖代谢和心肌线粒体损伤，延缓心肌纤维化进展。 |
| 英文摘要: |
| ABSTRACT Objective: To study the effect of mulberry leaf flavonoids on myocardial mitochondrial function and myocardial fibrosis in diabetic mice, and to explore the specific molecular mechanism of its effect. Methods: 45 ICR mice were randomly divided into 3 groups, namely normal group, model group and mulberry leaf flavonoid group. The model group and the mulberry leaf flavonoids group were intraperitoneally injected with alloxan saline solution to establish a diabetic model, and the mice in the normal group were intraperitoneally injected with saline. The diabetic mice in the mulberry leaf flavonoid group were treated with mulberry leaf flavonoids (1.0 g/kg/day) by gavage after the model was successfully established, and the normal group and the model group were treated with the same amount of normal saline. After 6 weeks of treatment, the levels of blood glucose, blood insulin, liver glycogen, liver hexokinase (HK) and pyruvate kinase (PK), myocardial mitochondria glutathione (GSH) and superoxide were measured and compared among the three groups of mice. Dismutase (SOD), total calcium and ATP content, and cardiac CD31, α-SMA and Collagen I mRNA expression. Results: During the study period, 3 mice in the model group and 1 mouse in the mulberry leaf flavonoid group died. After 6 weeks of treatment with mulberry leaf flavonoids, the blood sugar of diabetic mice was significantly reduced, the blood insulin level, liver glycogen, liver HK and liver PK content, and myocardial mitochondrial GSH, SOD, total calcium and ATP content were significantly increased (P<0.05). Myocardial fibrosis indicators: the expression of CD31 mRNA in the myocardium of diabetic mice was significantly increased after treatment with mulberry leaf flavonoids, but the expression of α-SMA and Collagen I mRNA was significantly decreased (P<0.05). Conclusion: Mulberry leaf flavonoids can significantly reduce blood sugar levels in diabetic mice, improve their glucose metabolism and myocardial mitochondrial damage, and delay the progression of myocardial fibrosis. |
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