| 韩田田,卫 丽,刘 虹,刘 娜,林博浩.布地奈德混悬液对哮喘幼鼠上皮-间充质转化和气道重塑的影响及机制[J].,2022,(7):1216-1219 |
| 布地奈德混悬液对哮喘幼鼠上皮-间充质转化和气道重塑的影响及机制 |
| Effect and Mechanism of Budesonide Suspension on Epithelial-mesenchymal Transition and Airway Remodeling in Asthmatic Young Mice |
| 投稿时间：2021-07-23 修订日期：2021-08-18 |
| DOI：10.13241/j.cnki.pmb.2022.07.004 |
| 中文关键词: 布地奈德混悬液 哮喘 幼鼠 上皮-间充质转化 气道重塑 肿瘤坏死因子-α 血红素加氧酶-1 |
| 英文关键词: Budesonide suspension asthma Young mice Epithelial-mesenchymal transition Airway remodeling Tumor necrosis factor-α Heme oxygenase-1 |
| 基金项目:陕西省国际科技合作计划项目（2018KW-041） |
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| 摘要 目的：探讨布地奈德混悬液对哮喘幼鼠上皮-间充质转化和气道重塑的影响及机制。方法：将哮喘幼鼠(n=36)随机平分为三组-模型组、布地奈德1组、布地奈德2组，三组分别给予雾化吸入生理盐水、布地奈德混悬液0.1 mg与布地奈德混悬液0.2 mg，1次/d，共14d，检测与观察幼鼠上皮-间充质转化和气道重塑变化情况。结果：布地奈德1组、布地奈德2组的支气管肺泡灌洗液白细胞总数与嗜酸性粒细胞总数都低于模型组(P<0.05)，布地奈德2组低于布地奈德1组(P<0.05)。布地奈德1组、布地奈德2组的血清肿瘤坏死因子(Tumor necrosis factor，TNF)-α、血红素加氧酶(heme oxygenase，HO)-1含量低于模型组(P<0.05)，布地奈德2组低于布地奈德1组(P<0.05)。布地奈德1组、布地奈德2组的支气管壁厚度(WAt/Pi)、支气管壁平滑肌细胞核数量(N/Pi)、支气管壁平滑肌厚度(WAm/Pi)都高于模型组，布地奈德2组高于布地奈德1组(P<0.05)。布地奈德1组、布地奈德2组的肺组织E-cadherin、NF-κB蛋白相对表达水平低于模型组(P<0.05)，布地奈德2组低于布地奈德1组(P<0.05)。结论：布地奈德混悬液在哮喘幼鼠的应用能抑制上皮-间充质转化和气道重塑，也可抑制TNF-α、HO-1的释放，减轻嗜酸性粒细胞与白细胞的浸润，从而发挥肺保护作用，且存在剂量依赖性。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects and mechanism of budesonide suspension on epithelial-mesenchymal transition and airway remodeling in asthmatic young rats. Methods: Asthmatic infant rats (n=36) were randomly divided into three groups-model group, budesonide 1 group, and budesonide 2 groups. The three groups were given aerosol inhalation of normal saline, budesonide suspension 0.1 mg, budesonide suspension 0.2 mg, 1 time/d, for a total of 14 days, detected and observed the changes of epithelial-mesenchymal transition and airway remodeling in the rats. Results: The total number of leukocytes and eosinophils in the bronchoalveolar lavage fluid of the budesonide group 1 and budesonide group 2 were lower than those of the model group (P<0.05), and the budesonide group 2 were lower than the budesonide group 1 (P<0.05). The serum tumor necrosis factor (TNF)-α and heme oxygenase (HO)-1 levels in budesonide group 1 and budesonide group 2 were lower than those in the model group(P<0.05), and the budesonide group 2 were lower than budesonide group 1(P<0.05). The bronchial wall thickness (WAt/Pi), the number of bronchial wall smooth muscle cell nuclei (N/Pi) and the bronchial wall smooth muscle thickness (WAm/Pi) of the budesonide group 1 and budesonide group 2 were higher than those of the model group, and the budesonide group 2 were higher than the budesonide group 1(P<0.05). The relative expression levels of E-cadherin and NF-κB protein in lung tissues of budesonide group 1 and budesonide group 2 were lower than those of the model group (P<0.05), and budesonide group 2 were lower than budesonide group 1(P<0.05). Conclusion: The application of budesonide suspension in asthmatic young mice can inhibit epithelial-mesenchymal transition and airway remodeling, and can also inhibit the release of TNF-α and HO-1, and reduce the infiltration of acidic granulocytes and leukocytes, thereby reduce the infiltration of acidic granulocytes and leukocytes. So as to play lung protective effect, and there are dose-dependent. |
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