| 刘 云,马 立,赵佳楠,聂婵娟,张玮洪.基于Wnt/β-catenin信号通路探讨miR-613对宫颈癌SiHa细胞增殖、迁移与侵袭的影响[J].,2022,(5):837-841 |
| 基于Wnt/β-catenin信号通路探讨miR-613对宫颈癌SiHa细胞增殖、迁移与侵袭的影响 |
| Investigate the Effects of miR-613 on the Proliferation, Migration and Invasion of Cervical Cancer SiHa Cells Based on Wnt/β-catenin Signaling Pathway |
| 投稿时间：2021-07-27 修订日期：2021-08-23 |
| DOI：10.13241/j.cnki.pmb.2022.05.008 |
| 中文关键词: 宫颈癌 miR-613 增殖 迁移 侵袭 Wnt/β-catenin信号通路 |
| 英文关键词: Cervical cancer miR-613 Proliferation Migration Invasion Wnt/β-catenin signaling pathway |
| 基金项目:河北省医学科学研究重点课题计划项目（ZD20160055） |
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| 中文摘要: |
| 摘要 目的：研究基于Wnt/β-catenin信号通路探讨微小RNA（miR）-613对宫颈癌SiHa细胞增殖、迁移与侵袭的影响。方法：体外培养宫颈癌SiHa细胞和正常宫颈上皮细胞H8，检测细胞中miR-613表达。根据转染miR-613 mimic浓度不同，将SiHa细胞分为0 μmol/L组，100 μmol/L和200 μmol/L组。MTT法检测细胞增殖情况，划痕实验检测细胞迁移能力，Transwell实验检测细胞侵袭能力。蛋白免疫印迹法检测miR-613表达对Wnt/β-catenin信号通路蛋白β-catenin、Vimentin、E-cadherin和MMP9表达的影响。结果：宫颈癌SiHa细胞中miR-613表达水平均显著低于正常宫颈上皮细胞H8，差异有统计学意义(P<0.05)。转染miR-613 mimic后，100 μmol/L、200 μmol/L 组SiHa细胞中miR-613表达水平显著上调，并且具有浓度依赖性(P<0.05)。与0 μmol/L组相比，100 μmol/L、200 μmol/L组的SiHa细胞增殖，迁移，侵袭能力均明显下降，并且具有浓度依赖性(P<0.05)。免疫印迹结果，与0 μmol/L组相比，100 μmol/L、200 μmol/L各浓度组的SiHa细胞Wnt/β-catenin信号通路蛋白β-catenin、Vimentin和MMP9表达显著下调，E-cadherin表达显著上调，并且具有浓度依赖性(P<0.05)。结论：miR-613能通过抑制Wnt/β-catenin信号通路抑制人宫颈癌细胞系SiHa细胞的增殖，迁移和侵袭。 |
| 英文摘要: |
| ABSTRACT Objective: To study the effect of microRNA (miR)-613 on the proliferation, migration and invasion of cervical cancer SiHa cells based on the Wnt/β-catenin signaling pathway. Methods: Cervical cancer SiHa cells and normal cervical epithelial cells H8 were cultured in vitro, and the expression of miR-613 in the cells was detected. According to the different concentrations of transfected miR-613 mimic, SiHa cells were divided into 0 μmol/L group, 100 μmol/L and 200 μmol/L group. Cell proliferation was detected by MTT assay, cell migration ability was detected by scratch assay, and cell invasion ability was detected by Transwell assay. Western blotting was used to detect the effects of miR-613 expression on the expression of Wnt/β-catenin signaling pathway proteins β-catenin, Vimentin, E-cadherin and MMP9. Results: The expression level of miR-613 in human cervical cancer cells SiHa was significantly lower than that in normal cervical epithelial cells H8, and the difference was statistically significant(P<0.05). After transfection of miR-613 mimic, the expression levels of miR-613 of SiHa cells in the 100 μmol/L and 200 μmol/L concentration groups were significantly up-regulated, and it was concentration-dependent(P<0.05). Compared with the 0 μmol/L group, the SiHa cells in the 100 μmol/L and 200 μmol/L concentration groups had significantly lower proliferation, migration and invasion abilities, and it was concentration-dependent(P<0.05). Western blot results showed that compared with the 0 μmol/L group, the expressions of Wnt/β-catenin signaling pathway proteins β-catenin, Vimentin and MMP9 were significantly down-regulated, and the expression of E-cadherin was significantly up-regulated in SiHa cells in the 100 μmol/L and 200 μmol/L concentration groups, and they were concentration-dependent(P<0.05). Conclusion: miR-613 can inhibit the proliferation, migration and invasion of human cervical cancer cell line SiHa cells by inhibiting the Wnt/β-catenin signaling pathway. |
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