| 吴 松,张文娟,崔茸茸,高芳芳,李迎楼.小檗碱对牙周炎大鼠的细胞因子水平、氧化应激和炎症性骨溶解的实验研究[J].,2022,(5):814-817 |
| 小檗碱对牙周炎大鼠的细胞因子水平、氧化应激和炎症性骨溶解的实验研究 |
| Study of Berberine on Cytokine Levels, Oxidative Stress and Inflammatory Osteolysis in Rats with Periodontitis |
| 投稿时间：2021-09-08 修订日期：2021-09-30 |
| DOI：10.13241/j.cnki.pmb.2022.05.003 |
| 中文关键词: 小檗碱 牙周炎 炎症 氧化应激 炎症性骨溶解 |
| 英文关键词: Berberine, Periodontitis, Inflammation, Oxidative stress, Inflammatory osteolysis |
| 基金项目:国家重点研发计划"国家质量基础的共性技术研究与应用"重点专项（2019YFF0216502） |
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| 中文摘要: |
| 摘要 目的：研究小檗碱对牙周炎大鼠的治疗效果，并探讨小檗碱治疗对牙周炎大鼠牙龈组织炎症细胞因子、氧化应激和炎症性骨溶解的影响。方法：45只SD大鼠被随机分为三组，即对照组、模型组和小檗碱治疗组。模型组和小檗碱治疗组大鼠通过涂抹牙周致病菌建立牙周炎模型，对照组大鼠涂抹生理盐水。小檗碱通过灌胃给予小檗碱进行治疗，对照组和模型组大鼠给予等量生理盐水。小檗碱治疗8周后，安乐死各组大鼠通过ELISA试剂盒测定各组大鼠牙龈组织中前列腺素E2（PEG2）、白介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、丙二醛（MDA）、过氧化氢脂质（LPO）、过氧化氢酶（CAT）、核因子-kB活化受体（RANKL）和骨保护素（OPG）含量。结果：与牙周炎模型组大鼠比较，小檗碱治疗组牙周炎大鼠牙龈指数和附着水平均显著降低（P<0.05）。小檗碱治疗8周后，牙周炎大鼠模型牙龈组织PEG2, IL-1β, TNF-α和IL-6等炎症细胞因子水平均显著下降（P<0.05），氧化应激指标MDA和LPO均显著下降（P<0.05），抗氧化应激指标CAT含量显著上升（P<0.05）。此外，小檗碱还显著降低牙周炎大鼠牙龈组织RANKL含量和RANKL/OPG比值（P<0.05），而显著提高牙周炎大鼠牙龈组织OPG含量（P<0.05）。结论：小檗碱对牙周炎大鼠具有较高的治疗效果，其机制可能与小檗碱治疗可降低牙周炎大鼠牙龈组织炎症细胞因子含量，降低氧化应激水平和缓解炎症性骨溶解有关。 |
| 英文摘要: |
| ABSTRACT Objective: To study the treatment effect of berberine on periodontitis rats, and to explore the effect of berberine treatment on inflammatory cytokines, oxidative stress and inflammatory osteolysis in the gingival tissue of rats with periodontitis. Methods: 45 SD rats were randomly divided into three groups, namely the control group, the model group and the berberine treatment group. The rats in the model group and the berberine treatment group were applied with periodontal pathogens to establish a periodontitis model, and the rats in the control group were applied with normal saline. Berberine was treated by intragastric administration of berberine, and rats in the control and model groups were given the same amount of normal saline. After 8 weeks of berberine treatment, rats in each group were euthanized by Elisa kit to determine the levels of prostaglandin E2(PEG2), interleukin-1β(IL-1β), and tumor necrosis factor-α (TNF-α) malondialdehyde (MDA), lipid hydrogen peroxide (LPO), catalase (CAT), nuclear factor-kB activated receptor (RANKL) and osteoprotegerin (OPG) content in the gingival tissue of each group of rats. Results: Compared with the periodontitis model group of rats, the gingival index and adhesion level of periodontitis rats in the berberine treatment group were significantly decreased (P<0.05). After 8 weeks of berberine treatment, the levels of inflammatory cytokines such as PEG2, IL-1β, TNF-α and IL-6 in the gingival tissue of the periodontitis rat model decreased significantly(P<0.05), and the oxidative stress indicators MDA and LPO Both were significantly decreased (P<0.05), and the content of anti-oxidative stress index CAT increased significantly (P<0.05). In addition, berberine also significantly reduced the RANKL content and RANKL/OPG ratio in the gingival tissue of rats with periodontitis (P<0.05), and significantly increased the OPG content in the gingival tissue of rats with periodontitis(P<0.05). Conclusion: Berberine has a high therapeutic effect on rats with periodontitis, and its mechanism may be related to the fact that berberine treatment can reduce the content of inflammatory cytokines in the gum tissue of rats with periodontitis, reduce the level of oxidative stress and relieve inflammatory osteolysis. |
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