| 薛 芬,薛姗姗,周翠红,刘江正,王化宁,吴 迪.氟西汀对慢性不可预见应激模型大鼠海马磷脂酰乙醇胺的影响[J].,2022,(4):606-609 |
| 氟西汀对慢性不可预见应激模型大鼠海马磷脂酰乙醇胺的影响 |
| Effect of Fluoxetine on Phosphatidylethanolamine in Hippocampus of Rats with Chronic Unpredictable Stress |
| 投稿时间：2021-05-28 修订日期：2021-06-24 |
| DOI：10.13241/j.cnki.pmb.2022.04.002 |
| 中文关键词: 磷脂酰乙醇胺 慢性不可预见应激模型 氟西汀 |
| 英文关键词: Phosphatidylethanolamine Chronic Unpredictable Mild Stress model Fluoxetine |
| 基金项目:国家自然科学基金项目(81571309；81904280) |
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| 中文摘要: |
| 摘要 目的：探讨氟西汀对慢性不可预见应激（Chronic Unpredictable Mild Stress，CUS）模型大鼠海马内磷脂酰乙醇胺（phosphatidylethanolamine，PE）组成的影响。方法：（1）将24只SD大鼠随机分为对照组（Sham）、模型组（CUS）和氟西汀组（Flx）。CUS组和Flx组均接受CUS造模，并且在造模后接受生理盐水（1 mL/kg）或氟西汀（10 mg/kg）腹腔注射，连续14天；Sham不进行CUS造模，但是每天接受腹腔注射生理盐水。随后处死大鼠，取海马进行脂质组学分析，比较各处理组海马总的PE和PE小分子相对丰度、不同碳链长度和含不同不饱和键PE的相对丰度差异。结果：（1）与CUS组相比，Sham组PE相对丰度明显减低，而Flx组明显增高（P<0.05）；（2）与Sham组相比，CUS组9个PE小分子相对丰度发生变化，PE（34:1e）、PE（36:1p）、PE（36:2）、PE（36:2p）、PE（36:4）、PE（38:2）、PE（38:4）和PE（40:7）共8个上调（P<0.05或0.01），PE（34:0p）下调（P<0.05），CUS组碳链长度为36的PE丰度上升（P<0.05），碳链长度为38的PE丰度下降（P<0.01），CUS组含0个不饱和键、4个不饱和键的PE丰度下调（P<0.01, P<0.05），而1个不饱和键的PE丰度上升（P<0.05）；（3）与CUS组相比，Flx组6个PE分子相对丰度减少，包括PE（34:1e）、PE（36:2）、PE（36:4）、PE（38:1p）、PE（38:6e）和PE（40:5p）（P<0.05或0.01），Flx组碳链长度为34的PE丰度下降（P<0.05），碳链长度为36的PE水平升高（P<0.05），Flx组含1个不饱和键的PE丰度下调（P<0.05），差异具有统计学意义。结论：氟西汀可以调节CUS模型大鼠海马的PE水平。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the impact of fluoxetine on the composition of phosphatidylethanolamine in hippocampus of rats with chronic unpredictable stress. Methods: 24 SD rats were randomly divided into control group (sham), model group (CUS) and fluoxetine group (FLX). Cus group and FLX group received CUS stimulus, and each group received intraperitoneal injection of normal saline (1 mL / kg) or fluoxetine (10 mg / kg) for 14 consecutive days; sham did not conduct CUS stimulus, but received intraperitoneal injection of normal saline every day. 24 h after the last stimulation, the rats were killed, and the hippocampus was taken for lipomics analysis. The relative concentrations of total PE and different molecules, different carbon chain length and unsaturated bonds of PE in the hippocampus of each group were compared. Results: (1) Compared to CUS group, the relative concentrations of PE in the Sham group was significantly reduced, while the Flx group was significantly increased（P<0.05）; (2) Compared to Sham group, there was significant difference on the relative concentrations of 9 molecules of PE, PE (34: 1e), PE (36: 1p), PE (36: 2), PE (36: 2p), PE (36: 4), PE (38: 2), PE (38: 4) and PE (40: 7) was increased and PE (34: 0p) was decreased in CUS group（P<0.05 or 0.01）, the concentrations of PE with a carbon chain length of 36 in the CUS group was increased（P<0.05）, and the concentrations of PE with a carbon chain length of 38 were decreased（P<0.01）, the concentrations of PE with 0 and 4 unsaturated bonds decreased（P<0.01, P<0.05）, while the PE concentrations of 1 unsaturated bond increased in CUS group（P<0.05）; (3) Compared to CUS group, there was significant difference in Flx group on the relative concentrations of PE composition by molecules, PE (34: 1e), PE (36: 2), PE (36: 4), PE (38: 1p ), PE (38: 6e) and PE (40: 5p) were increased（P<0.05 or 0.01）; the concentrations of PE with the carbon chain length of 34 were decreased in Flx group （P<0.05）, and the carbon chain length of 36 were increased（P<0.05）; the concentrations of PE with 1 unsaturated bond were decreased in Flx group（P<0.05）. Conclusion: Fluoxetine can regulate the level of PE in hippocampus of CUS model rats. |
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