| 董霏雪,孙 河,王丽媛,赵 爽,李 阳,刘清娇,刘 旭.通窍明目IV号对青光眼模型大鼠视神经保护的机制研究[J].,2022,(2):237-243 |
| 通窍明目IV号对青光眼模型大鼠视神经保护的机制研究 |
| Mechanism of TongQiaoMingMu IV on the Mechanism of Optic Nerve Protection in Rats of Glaucoma Model |
| 投稿时间：2021-06-27 修订日期：2021-07-23 |
| DOI：10.13241/j.cnki.pmb.2022.02.007 |
| 中文关键词: 通窍明目IV号 青光眼 Bcl-2 P53 Caspase |
| 英文关键词: TongQiaoMingMu IV Glaucoma Bcl-2 P53 Caspase |
| 基金项目:国家自然科学基金面上项目（81674029）；黑龙江省自然科学基金项目（QC2018115）；黑龙江省中医药科研项目（ZHY-031）；黑龙江中医药大学科研基金项目（201510） |
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| 中文摘要: |
| 摘要 目的：观察不同浓度通窍明目IV号对青光眼模型大鼠凋亡相关蛋白表达的干预作用。方法：将42只SD大鼠随机分为空白组，模型组，通窍明目4号低、中、高剂量组和神经营养剂组，共计6组，每组7只。经眼球前房角膜缘注射卡波姆复制高眼压模型，成模21天后灌胃给药，给药期4周。采用HE染色观察视网膜形态及视神经节胞、免疫组化法测定BCL-2、Bax、P53蛋白表达，用Western Blot法测定Caspase-3、Caspase-9蛋白表达。结果：与空白组对比，模型组视网膜Bax与p53阳性表达显著升高（P<0.01）；与模型组比较，神经营养剂组与通窍明目IV号高、中剂量组视网膜Bax与p53蛋白的阳性表达显著降低（P<0.01)。与空白组比较，模型组视网膜Bcl-2阳性表达显著降低（P<0.01）；与模型组比较，神经营养剂组与通窍明目IV号各剂量组视网膜Bcl-2的阳性表达有不同程度的提高。模型组大鼠caspase-3、caspase-9的蛋白表达水平均高于空白组（P<0.01）；与模型组比较，神经营养剂组与通窍明目IV号各剂量组大鼠视网膜Caspase3及caspase-9表达水平明显降低（P<0.01）。结论：通窍明目IV号可能通过下调Bax、P53表达，促进Bcl-2表达升高、抑制Caspase-3、Caspase-9激活凋亡途径，从而对青光眼视神经起到保护作用。 |
| 英文摘要: |
| ABSTRACT Objective: To observe the intervention effects of different concentrations of TongQiaoMingMu IV on the expression of apoptosis-related proteins in glaucoma model rats. Methods: 42 SD rats were randomly divided into control group(n=7), model group (n=7), TongQiaoMingMu IV low dose group (n=7), medium dose group (n=7), high dose group (n=7) and neurotrophic group (n=7). The model of high intraocular pressure was established by injecting carbomer through the limbal of the anterior chamber of the eyeball. 21 days later, the drug was intragastrically administered for 4 weeks. The morphology of retina and optic ganglion cells were observed by HE staining. The expression of BCL-2, Bax and p53 protein was detected by immunohistochemical method, and the expression of Caspase-3 and Caspase-9 protein was determined by WesternBlot method. Results: Compared with the blank group, the positive expression of Bax and p53 in the model group increased significantly, while the positive expression of Bax and p53 protein decreased significantly in the neurotrophic group and TongQiaoMingMu IV high and medium dose groups compared with the model group. Compared with the blank group, the positive expression of Bcl-2 in the model group decreased significantly, and compared with the model group, the positive expression of Bcl-2 in the neurotrophic group and TongQiaoMingMu IV group increased in different degrees. The protein expression levels of caspase-3 and caspase-9 in the model group were higher than those in the control group, and the expression levels of Caspase3 and caspase-9 in the retina of rats in the neurotrophic group and TongQiaoMingMu IV group were significantly lower than those in the model group (P＜0.01). Conclusion: TongQiaoMingMu IV may protect the optic nerve of glaucoma by down-regulating the expression of Bax and p53, increasing the expression of Bcl-2 and inhibiting the activation of apoptosis pathway of Caspase-3 and Caspase-9. |
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