| 张新燕,韦 东,胡 明,郑 若,孔香波.象皮生肌膏对肛瘘术后模型大鼠炎症因子、p38/MK2信号通路和创面组织VEGF、bFGF表达的影响[J].,2021,(17):3242-3246 |
| 象皮生肌膏对肛瘘术后模型大鼠炎症因子、p38/MK2信号通路和创面组织VEGF、bFGF表达的影响 |
| Effects of Xiangpi Shengji Ointment on Inflammatory Factors, p38/MK2 Signaling Pathway and Expressions of VEGF and bFGF in Wound Tissue of Rats with Anal Fistula |
| 投稿时间：2021-02-22 修订日期：2021-03-17 |
| DOI：10.13241/j.cnki.pmb.2021.17.009 |
| 中文关键词: 象皮生肌膏 肛瘘术后模型大鼠 炎症因子 p38/MK2信号通路 血管内皮生长因子 碱性成纤维细胞生长因子 |
| 英文关键词: Xiangpi Shengji ointment Anal fistula model rats Inflammatory factors p38/MK2 signaling pathway Vascular endothelial growth factor Basic fibroblast growth factor |
| 基金项目:海南省自然科学基金项目（20168324） |
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| 中文摘要: |
| 摘要 目的：探讨象皮生肌膏对肛瘘术后模型大鼠炎症因子、p38/MK2信号通路和创面组织血管内皮生长因子（VEGF）、碱性成纤维细胞生长因子（bFGF）表达的影响。方法：选择雄性SD大鼠60只，按随机数字表法分为象皮生肌膏组、模型组和假手术组，每组20只，象皮生肌膏组和模型组进行肛瘘术后创面造模，分别给予象皮生肌膏治疗及普通换药治疗。假手术组构建创面后滴加生理盐水造模，给予普通换药治疗。统计治疗后3 d、7 d、14 d创面愈合率、创面水肿积分；对比治疗后14 d血清白细胞介素-1（IL-1）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）水平和治疗后3 d、14 d创面组织VEGF、bFGF、p38、MK2、磷酸化p38（p-p38）、磷酸化MK2（p-MK2）表达。结果：治疗后7d、14d象皮生肌膏组创面愈合率高于模型组，但低于假手术组（P＜0.05）；治疗后7 d、14 d假手术组创面水肿积分低于象皮生肌膏组，且象皮生肌膏组低于模型组（P＜0.05）。治疗后14 d象皮生肌膏组血清IL-1、IL-6、TNF-?琢水平低于模型组，但高于假手术组（P＜0.05）。治疗后14 d象皮生肌膏组创面组织VEGF、bFGF水平高于模型组，但低于假手术组（P＜0.05）。治疗后14 d象皮生肌膏组创面组织p38、MK2蛋白水平低于模型组，但高于假手术组，p-p38、p-MK2蛋白水平高于模型组，但低于假手术组（P＜0.05）。结论：象皮生肌膏可以减轻大鼠肛瘘术后创面水肿程度，促进创面愈合。其主要机制可能与象皮生肌膏可降低大鼠血清炎症因子水平，抑制p38/MK2信号通路，促进VEGF和bFGF表达有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of Xiangpi Shengji ointment on inflammatory factors, p38/MK2 signaling pathway and the expression of vascular endothelial growth factor(VEGF) and basic fibroblast growth factor (bFGF) in wound tissue of rats after anal fistula operation. Methods: Sixty male SD rats were selected, they were randomly divided into Xiangpi Shengji ointment group, model group and sham operation group with 20 rats in each group. The wound models of the two groups were made after anal fistula operation, and treated with Xiangpi Shengji ointment and common dressing change respectively. The sham operation group was treated with normal saline after wound construction. The wound healing rate and wound edema score were calculated on 3 d, 7 d and 14 d after treatment, the levels of serum interleukin-1 (IL-1), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) on the 14 d after treatment and the expressions of VEGF, bFGF, p38, MK2, phosphorylated p38 (p-p38) and phosphorylated MK2 (p-MK2) on the 3 d and 14th day after treatment were compared. Results: The wound healing rate of the Xiangpi Shengji ointment group was higher than that of the model group, but lower than that of the sham operation group(P<0.05). The wound edema score of the sham operation group was lower than that of the Xiangpi Shengji ointment group, and the Xiangpi Shengji ointment group was lower than that of the model group (P<0.05). 14 d after treatment, the levels of serum IL-1, IL-6 and TNF-α in the Xiangpi Shengji ointment group were lower than those in the model group, but higher than those in the sham operation group (P<0.05). 14 d after treatment, the levels of VEGF and bFGF in Xiangpi Shengji ointment group were higher than those in model group, but lower than those in sham operation group (P<0.05). 14 d after treatment, the protein levels of p38 and MK2 in Xiangpi Shengji ointment group were lower than those in the model group, but higher than those in the sham operation group, and the protein levels of p-p38, p-MK2 in Xiangpi Shengji ointment group were higher than those in the model group, but lower than those in the sham operation group(P<0.05). Conclusion: Xiangpi Shengji ointment can reduce the degree of postoperative wound edema and promote wound healing. The main mechanism may be that Xiangpi Shengji ointment can reduce the level of serum inflammatory factors, inhibit p38/MK2 signaling pathway, and promote the expression of VEGF and bFGF. |
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