| 艾尼瓦尔·吾拉木,帕丽扎提·阿不都热依木,刘晓萱,卢 婷,马文婷.HIV/AIDS患者抗病毒治疗前HIV-1耐药情况调查及外周血CD8+T细胞CD38表达水平研究[J].,2021,(16):3092-3096 |
| HIV/AIDS患者抗病毒治疗前HIV-1耐药情况调查及外周血CD8+T细胞CD38表达水平研究 |
| Investigation of HIV-1 Drug Resistance in HIV/AIDS Patients before Antiviral Treatment and Study on the Expression Level of CD8+T Cells CD38 in Peripheral Blood |
| 投稿时间：2021-02-03 修订日期：2021-02-27 |
| DOI：10.13241/j.cnki.pmb.2021.16.019 |
| 中文关键词: 人免疫缺陷病毒 艾滋病 耐药 影响因素 CD8+T细胞CD38 CD4+T细胞 |
| 英文关键词: Human immunodeficiency virus AIDS Drug resistance Influencing factors CD8+T cells CD38 CD4+ T cells |
| 基金项目:新疆维吾尔自治区自然科学基金项目（2017D01C17） |
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| 中文摘要: |
| 摘要 目的：分析人免疫缺陷病毒/艾滋病（HIV/AIDS）患者抗病毒治疗前HIV-1耐药以及影响因素，探讨HIV/AIDS患者外周血CD8+T细胞CD38表达（CD8+CD38+T淋巴细胞百分比）与CD4+T淋巴细胞计数的相关性。方法：选择2016年3月至2019年12月我院接诊的442例HIV/AIDS患者（HIV/AIDS组）和163例同期于我院进行体检的健康志愿者（对照组），HIV/AIDS组扩增pol基因，进行HIV-1基因耐药分析，检测CD8+CD38+T淋巴细胞百分比、CD4+T淋巴细胞计数、CD8+T淋巴细胞计数。分析HIV/AIDS患者HIV-1耐药的影响因素，分析CD8+CD38+T淋巴细胞百分比与CD4+T淋巴细胞计数、CD8+T淋巴细胞计数相关性。结果：HIV/AIDS组442例HIV/AIDS患者中376例获得HIV-1 pol基因序列，HIV-1耐药35例，耐药率9.31%（35/376）。单因素分析结果显示耐药组和非耐药组在年龄、文化程度、感染途径、HIV病毒载量方面差异有统计学意义（P＜0.05）。多因素Logistic回归分析结果显示同性性传播、注射吸毒、高HIV病毒载量是HIV/AIDS患者抗病毒治疗前HIV-1耐药的危险因素（P＜0.05）。HIV/AIDS组外周血CD4+T淋巴细胞计数、CD8+T淋巴细胞计数低于对照组（P＜0.05），CD8+CD38+T淋巴细胞百分比高于对照组（P＜0.05）。CD8+CD38+T淋巴细胞百分比与CD4+T淋巴细胞计数、CD8+T淋巴细胞计数呈负相关（P＜0.05）。结论：抗病毒治疗前HIV/AIDS患者存在一定HIV-1耐药率，传播途径、HIV-1病毒载量与HIV-1耐药有关。CD8+T细胞表面CD38过表达与HIV/AIDS 患者CD4+T T细胞的过度消耗有关。 |
| 英文摘要: |
| ABSTRACT Objective: To analyze HIV-1 drug resistance and influencing factors before antiviral therapy in human immunodeficiency virus /AIDS (HIV/AIDS) patients, and to investigate the correlation between CD8+T cells CD38 expression (percentage of CD8+CD38+T lymphocytes) and CD4+T lymphocyte count in peripheral blood of HIV/AIDS patients. Methods: 442 HIV/AIDS patients (HIV/AIDS group) and 163 healthy volunteers (control group) who were admitted to our hospital from March 2016 to December 2019 were selected. The HIV/AIDS group amplified pol gene, and the drug resistance of HIV-1 gene were analyzed. The percentage of CD8+CD38+T lymphocytes, CD4+T lymphocyte count and CD8+ T lymphocyte count were detected. The influencing factors of HIV-1 drug resistance in HIV/AIDS patients were analyzed, and the correlation between the percentage of CD8+CD38+T lymphocytes and CD4+T lymphocyte count and CD8+T lymphocyte count were analyzed. Results: In the HIV/AIDS group, 376 cases of the 442 cases of HIV/AIDS patients obtained HIV-1 Pol gene sequence, and 35 cases were HIV-1 drug resistance, with drug resistance rate of 9.31% (35/376). Univariate analysis results showed that the drug-resistant group and non-drug-resistant group had statistically significant differences in age, educational level, route of infection and HIV viral load (P＜0.05). Multivariate Logistic regression analysis showed that homosexual sexual transmission, injecting drug abuse and high HIV viral load were the risk factors for HIV-1 drug resistance in HIV/AIDS patients before antiviral treatment (P＜0.05). The CD4+T lymphocyte count and CD8+T lymphocyte count in peripheral blood of HIV/AIDS group were lower than those of control group (P＜0.05), and the percentage of CD8+CD38+T lymphocytes was higher than that of control group (P＜0.05). The percentage of CD8+CD38+T lymphocytes was negatively correlated with CD4+T lymphocyte count and CD8+T lymphocyte count (P＜0.05). Conclusion: There is a certain drug resistance rate of HIV-1 in HIV/AIDS patients before antiviral treatment. Transmission route, HIV-1 viral load are related to HIV-1 drug resistance. Overexpression of CD38 on CD8+T cells is associated with excessive depletion of CD4+TT cells in HIV/AIDS patients. |
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