| 段鸣锐,黄佩芝,欧官用,方 州,沈辉帆,乔新宇,赵炜疆.APPswe小鼠β淀粉样斑块与胶质细胞激活相关性研究[J].,2021,(15):2819-2822 |
| APPswe小鼠β淀粉样斑块与胶质细胞激活相关性研究 |
| Correlation Study on the Roles of Glial Cell Activation in APPswe Mice |
| 投稿时间：2021-01-06 修订日期：2021-01-29 |
| DOI：10.13241/j.cnki.pmb.2021.15.004 |
| 中文关键词: 阿尔兹海默症 APPswe小鼠 β淀粉样斑块 神经炎症 GFAP Iba-1 |
| 英文关键词: Alzheimer's Disease (AD) APPswe mouse β-Amyloid plaque Neuroinflammation GFAP Iba-1 |
| 基金项目:国家自然科学基金面上项目 (81471279；81171138) |
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| 中文摘要: |
| 摘要 目的：通过检测阿尔茨海默病（Alzheimer's Disease，AD）APPswe转基因小鼠前额叶皮质（prefrontal cortex, PFC）中β-淀粉样蛋白（β-amyloid, Aβ）与胶质纤维酸性蛋白(glial fibrillary acidic protein, GFAP)和离子钙结合衔接分子1（Ionized calcium binding adaptor molecule-1，Iba-1）的表达相关性，进一步明确AD中β淀粉样斑块在神经胶质细胞激活中的重要作用。方法：选取9只12月龄APPswe AD小鼠，使用免疫组织化学法检测PFC中Aβ、GFAP以及Iba-1表达，并分析Aβ与GFAP和Iba-1水平的相关性；使用免疫荧光双标法分别评价不同大小Aβ斑块分别与GFAP和Iba-1的共染情况。结果：免疫组织化学结果显示，AD小鼠前额叶皮质中Aβ水平高，则GFAP和Iba-1信号水平也较高；反之，Aβ水平低，GFAP和Iba-1的表达水平也较低。Pearson相关性分析结果表明Aβ水平与GFAP（R=0.6677，P<0.05）和Iba-1（R=0.8257，P<0.05）的水平呈正相关，且与GFAP相比，Iba-1显示出与Aβ水平更高的表达相关性。与免疫组织化学结果一致，免疫荧光双标法结果亦表明小鼠PFC中较大的Aβ斑块所在区域GFAP或Iba-1荧光信号强度及范围亦大于较小的Aβ斑块所在区域GFAP或Iba-1荧光信号强度。结论：AD小鼠前额叶皮质中Aβ水平与GFAP和Iba-1的表达量呈正相关，表明Aβ形成在神经胶质细胞的激活中发挥重要作用。 |
| 英文摘要: |
| ABSTRACT Objective: To verify the relationship between β-amyloid (Aβ) plaques and glial cell activation by testing the correlation between Aβ and glial fibrillary acidic protein (GFAP) or ionized calcium binding adaptor molecule-1 (Iba-1) in the prefrontal cortex (PFC) of an Alzheimer's Disease (AD）APPswe mouse model. Methods: Nine 12-month-old APPswe AD mice were included. The expression of Aβ, GFAP, and Iba-1 in the PFC was detected by immunohistochemistry, and the correlation of Aβ with either GFAP or Iba-1 level was analyzed. The co-existence of different sized Aβ plaques with either GFAP or Iba-1 was detected by double-immunofluorescence labeling assay. Results: Immunohistochemical results showed that higher levels of Aβ in the PFC of AD mice were associated with higher levels of GFAP and Iba-1. On the contrary, lower levels of Aβ were associated with lower levels of GFAP and Iba-1 expression. Pearson correlation analysis showed that Aβ level was positively correlated with that of GFAP (R=0.6677, P<0.05) and Iba-1 (R=0.8257, P<0.05), respectively. Immunofluorescence double labeling results also demonstrated that the region with large sized Aβ plaques showed a higher fluorescence signal intensity of both GFAP and Iba-1 in the PFC of AD mice when compared to that with small sized Aβ plaques. Conclusion: The level of Aβ was positively correlated with those of GFAP and Iba-1 in the PFC of AD mice, suggesting that Aβ formation plays an important role in neuroinflammation induced by the activation of glial cells. |
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