| 钟士杰,邓 堂,林开文,廖 勇,魏胜超,刘华江,史键山,云宏芳,金桂云.车前草提取物对缺氧性肺动脉高压SD大鼠模型肺动脉压力、肺功能及炎症因子的影响[J].,2021,(11):2035-2039 |
| 车前草提取物对缺氧性肺动脉高压SD大鼠模型肺动脉压力、肺功能及炎症因子的影响 |
| Plantain Extract on SD Rat Model of Hypoxic Pulmonary Hypertension Pulmonary Artery Pressure, Lung Function and the Influence of Inflammatory Factors |
| 投稿时间：2021-01-06 修订日期：2021-01-28 |
| DOI：10.13241/j.cnki.pmb.2021.11.007 |
| 中文关键词: 缺氧性肺动脉高压 HPH 车前草提取物 肺动脉压力 肺功能 |
| 英文关键词: Hypoxic pulmonary hypertension HPH Plantain Fasudil Pulmonary artery pressure Lung function |
| 基金项目:国家自然科学基金项目（31560267） |
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| 中文摘要: |
| 摘要 目的：探讨车前草提取物对缺氧性肺动脉高压(HPH)SD大鼠模型肺动脉压力、肺功能及炎症改变的效果。方法：随机选取18只SD雄性大鼠分为3组，正常对照组（Control组）、缺氧性肺动脉高压（HPH组）、车前草干预组（PW组），每组各6只。观察并记录各组大鼠日常状况，于第21天测定大鼠肺动脉压力、肺功能后处死各组6只大鼠，获取大鼠肺组织标本，左肺泡灌洗液予Elisa试剂盒检测各组ICAM-1、TGF-β、MMP-9因子含量，右肺上中下叶各取一块组织行苏木精-伊红染色，其余肺组织研磨匀浆后予Elisa试剂盒检测HIF-1α含量。结果：用PW干预HPH大鼠，可降低SD大鼠的平均肺动脉压力，提高0.4 sFEV、FVC、0.4sFEV/FVC、MMEF等肺功能指标，降低肺泡灌洗液中ICAM-1、TGF-β、MMP-9炎症因子水平及肺组织中HIF-1a水平，气道及肺动脉管腔扩大，管壁变薄，炎性细胞浸润减少。结论：车前草提取物能降低HIF-1a水平，减轻HPH SD大鼠的炎症反应，降低肺动脉压力，提升肺功能，改善肺动脉及气道重塑。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of plantain extract on pulmonary artery pressure, pulmonary function and inflammation in SD rat model of hypoxic pulmonary hypertension (HPH). Methods: Eighteen SD male rats were randomly selected and divided into 3 groups, the normal control group (Control group), hypoxic pulmonary hypertension (HPH group), and plantain intervention group (PW group), each with 6 rats. Observe and record the daily status of the rats in each group. On the 21st day, the pulmonary artery pressure and pulmonary function of the rats were measured and then 6 rats in each group were sacrificed. The lung tissue samples of the rats were obtained. The left alveolar lavage fluid was tested by Elisa kit for each group. ICAM-1, TGF-β, MMP-9 factors content, a piece of tissue from each of the upper, middle, and lower lobes of the right lung was stained with hematoxylin and eosin, and the remaining lung tissues were ground and homogenized and then tested for HIF-1α content with Elisa kit. Results: Intervening HPH rats with PW can reduce the average pulmonary artery pressure of SD rats, increase lung function indexes such as 0.4 sFEV, FVC, 0.4 sFEV/FVC, MMEF, and reduce ICAM-1, TGF-β, and ICAM-1 in alveolar lavage fluid. The level of MMP-9 inflammatory factors and the level of HIF-1α in lung tissues, the airway and pulmonary artery lumens are enlarged, the tube wall becomes thinner, and the infiltration of inflammatory cells is reduced. Conclusion: Plantain extract can reduce the level of HIF-1α, reduce inflammation in HPH SD rats, reduce pulmonary artery pressure, enhance lung function, and improve pulmonary artery and airway remodeling. |
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