文章摘要
聂雨泽,王 燕,杨 帆,杨 艳,孙 薇.天麻素通过下调Nav1.6通道表达缓解糖尿病神经病理性疼痛的研究[J].,2021,(4):629-633
天麻素通过下调Nav1.6通道表达缓解糖尿病神经病理性疼痛的研究
Gastrodin Alleviated Diabetic Neuropathic Pain via Downregulating the Expression of Nav1.6 in Rats
投稿时间:2020-07-23  修订日期:2020-08-18
DOI:10.13241/j.cnki.pmb.2021.04.006
中文关键词: 糖尿病神经病理性疼痛  天麻素  Nav1.6通道  背根神经节
英文关键词: Diabetic neuropathic pain  Gastrodin  Nav1.6 channel  Dorsal root ganglion
基金项目:唐都医院科技创新发展基金项目(2019QYTS005);陕西省重点研发计划项目(2020SF-164);唐都医院苗子人才资助计划项目;国家自然科学基金项目(31300919)
作者单位E-mail
聂雨泽 空军军医大学基础医学院 陕西 西安710038 1365005524@qq.com 
王 燕 空军军医大学第二附属医院疼痛生物医学研究所 陕西 西安710038  
杨 帆 空军军医大学第二附属医院疼痛生物医学研究所 陕西 西安710038  
杨 艳 空军军医大学第二附属医院疼痛生物医学研究所 陕西 西安710038  
孙 薇 空军军医大学第二附属医院疼痛生物医学研究所 陕西 西安710038  
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中文摘要:
      摘要 目的:探究天麻素对 Ⅱ 型糖尿病神经病理性痛的镇痛作用以及天麻素对背根神经节Nav1.6通道的表达调控作用。方法:将60只雄性SD大鼠随机分为空白对照组、糖尿病组和天麻素处理组(10 mg?kg-1?d-1)。通过高脂饮食喂养4周,低剂量腹腔注射STZ (30 mg?kg-1) 的方法构建 Ⅱ 型糖尿病神经病理性痛大鼠模型,利用痛行为学检测观察各组大鼠的机械刺激足缩反应阈值变化,采用免疫荧光组织化学及Western blot方法观察各组大鼠背根神经节上Nav1.6通道的表达变化。结果:与空白对照组相比,糖尿病模型大鼠出现显著的机械刺激疼痛阈值下降 (P<0.05),且模型组大鼠背根神经节神经元上的Nav1.6通道表达上调 (P< 0.05)。与糖尿病组相比,连续腹腔注射天麻素 3天、7天、14天后,模型动物的疼痛明显缓解 (P<0.05),另外天麻素可以翻转背根神经节上Nav1.6通道的高表达 (P<0.05)。结论:天麻素可能通过降低Nav1.6通道的表达来缓解 Ⅱ 型糖尿病神经病理性疼痛,从而为天麻素缓解糖尿病神经病理性疼痛提供新的理论依据。
英文摘要:
      ABSTRACT Objective: To investigate the inhibitory effect of gastrodin on allodynia in diabetic neuropathic pain and its mechanisms. Methods: 144 male SD rats were randomly divided into three groups: control group, diabetes group and gastrodin treatment group (10 mg?kg-1?d-1). Type Ⅱ Diabetic rats were fed high fat diet for 4 weeks and intraperitoneally injected with low-dose streptozotocin (STZ) (30 mg?kg-1). The paw withdrawal mechanical threshold (PWMT) was detected to reflect the pain behavioral changes. The expression of Nav1.6 was observed by immunofluorescence histochemistry and Western blot analysis. Results: Compared with control rats, type Ⅱ diabetic rats showed significant pain sensitization and lower PWMT (P<0.05). Upregulated expression of Nav1.6 channel was detected on dorsal root ganglion (DRG) neurons from these painful diabetic rats (P<0.05). After continuous injection of gastrodin for 3 days, 7 days and 14 days, the allodynia of diabetic rats was significantly relieved (P<0.05). Moreover, gastrodin could reverse the overexpression of Nav1.6 on dorsal root ganglion neurons (P<0.05). Conclusion: Gastrodin could alleviate diabetic neuropathic pain by downregulating the overexpression of Nav1.6. This research may provide new data for clinical therapy in diabetic neuropathic pain.
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