| 高崇婷,桂定坤,汪年松,谢 玲,苏 君,叶 丹.黄芪皂苷Ⅱ对STZ诱导的糖尿病大鼠的肾脏保护作用研究[J].,2020,(23):4401-4406 |
| 黄芪皂苷Ⅱ对STZ诱导的糖尿病大鼠的肾脏保护作用研究 |
| Renal Protective Effect of Astragaloside-Ⅱ on STZ-induced Diabetic Rats |
| 投稿时间：2020-04-23 修订日期：2020-05-18 |
| DOI：10.13241/j.cnki.pmb.2020.23.001 |
| 中文关键词: 糖尿病肾病 黄芪皂苷Ⅱ 足细胞 凋亡 |
| 英文关键词: Diabetic nephrology Astragaloside-Ⅱ Podocyte Apoptosis |
| 基金项目:国家自然科学基金面上项目（81774052，81573738) |
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| 中文摘要: |
| 摘要 目的：研究黄芪皂苷Ⅱ（Astragaloside-Ⅱ, AS-Ⅱ）对链脲佐菌素（Streptozocin，STZ）诱导的糖尿病肾病（Diabetic nephrology，DN）大鼠肾脏的保护作用。方法：将8周龄雄性SD（Sprague-Dawley）大鼠随机选取5只作为正常对照组，其余大鼠予腹腔注射55 mg/kg剂量STZ建立糖尿病模型。造模成功的大鼠随机分为模型组与AS-Ⅱ 治疗组，每组5只。AS-Ⅱ 治疗组予AS-Ⅱ 3.2mg/(kgod）连续口服灌胃治疗9周，同时正常对照组和模型组给予等量生理盐水溶液灌胃。第9周末，收集大鼠24 h尿液测定尿微量白蛋白浓度，留取肾脏组织观察肾脏病理改变并检测肾组织中足细胞裂孔隔膜蛋白Nephrin、WT1和caspase-3的表达。结果：与糖尿病模型组比较， AS-Ⅱ 治疗组DN大鼠肾脏病理损伤明显改善， caspase-3表达明显减少，Nephrin和WT1表达增加，尿蛋白排泄减轻( P＜0. 05)。结论：AS-Ⅱ 治疗可改善DN大鼠肾脏足细胞凋亡，降低尿蛋白，具有肾脏保护作用。 |
| 英文摘要: |
| ABSTRACT Objective: To study the effects of Astragaloside-Ⅱ(AS-Ⅱ) on renal protection in streptozotocin (STZ) induced type 1 diabetic rats. Methods: Five male Sprague-Dawley (SD) rats aged 8 weeks were randomly selected as the normal control group. The rest of the rats were induced by intraperitoneal injection of STZ (55 mg/kg) to establish the model of type 1 diabetes rats. The diabetic rats were randomly divided into diabetic model group and AS-Ⅱ treatment group (n=5). AS-Ⅱ treatment group was given 3.2 mg/(kg?d) solution by gavage for 9 weeks. Normal control group and diabetes model group were given the same volume by gavage for 9 weeks. At the end of the 9th week, urine albumin to creatinine ratio (ACR) and renal histopathology were examined, immunohistochemistry was used to detect the protein expression of Nephrin, WT1 and caspase-3. Results: Compared with diabetes model group, ACR and the renal histopathology was obviously improved, moreover, the expression of Nephrin and WT1 were up-regulated and the expression of caspase-3 were down-regulated in AS-Ⅱ treatment group. Conclusion: Therefore, AS-Ⅱ ameliorates podocyte apoptosis in STZ-induced type 1 diabetic rats, alleviate the pathological injury of the kidney, reduce ACR. has a protective effect on the kidney of STZ-induced type 1 diabetic rats, which can delay the progression of diabetic nephropathy. |
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