| 周玉弟,张 杰,田伟千,崔耀梅,解 珂.电针对术后认知功能障碍大鼠认知功能及海马α7nAChR受体表达的影响[J].,2020,(20):3815-3821 |
| 电针对术后认知功能障碍大鼠认知功能及海马α7nAChR受体表达的影响 |
| Effect of Electroacupuncture on the Cognitive Function and Expression of α7nAChR Receptor in the Hippocampus of Rats with Postoperative Cognitive Dysfunction |
| 投稿时间：2020-05-29 修订日期：2020-06-25 |
| DOI：10.13241/j.cnki.pmb.2020.20.003 |
| 中文关键词: 电针 术后认知功能障碍 α7烟碱型乙酰胆碱受体 |
| 英文关键词: Electroacupuncture Postoperative cognitive dysfunction α7nAChR |
| 基金项目:国家自然科学基金项目（NSFC 81673741） |
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| 中文摘要: |
| 摘要 目的：探讨电针对术后认知功能大鼠认知功能及海马α7型神经烟碱胆碱能受体(nicotinic acetylcholine receptor，α7nAChR)表达含量的影响。方法：选择120只雄性SD大鼠，将其随机分为以下5组：对照组(Ctrl组)、手术组(Op组)、电针组(EA)、α7nAChR抑制剂 (EA+α-BGT)组、α7nAChR激动剂 (PHA-543,613组)，每组12只，每组又分为1 d和3 d两个亚组。采用Morris水迷宫检测认知功能，ELISA检测血清肿瘤坏死因子(Tumor necrosis factor，TNF)和白细胞介素-1β(interleukin-1β，IL-1β)、高迁移率族蛋白1(High mobility group box 1，HMGB-1)含量，RT-PCR检测海马TNF-α和IL-1β、HMGB-1 m-RNA表达，蛋白印迹法检测海马α7nAChR表达，甲苯胺蓝法检测海马CA1区肥大细胞活化情况，Tunel法检测海马CA1区细胞凋亡情况。结果：与Op组相比，EA组、PHA-543,613组术后第1 d、第3 d逃避潜伏期显著缩短，穿越平台次数增加 (P<0.05)；与EA组相比，EA+α-BGT组术后第1 d、第3 d逃避潜伏期显著延长，穿越平台次数明显减少(P<0.05)。与Op组相比，EA组、PHA-543,613组鼠术后第1 d、第3 d血清TNF-α和IL-1β、HMGB-1含量显著降低，海马TNF-α和IL-1β、HMGB-1 m-RNA表达亦明显下调，海马α7nAChR蛋白含量表达上调、海马CA1区肥大细胞活化数目、Tunel阳性细胞数目减少，两组比较差异有统计学意义(P<0.05)。与EA组相比，EA+α-BGT组大鼠术后第1 d、第3 d血清TNF-α和IL-1β、HMGB-1含量增加，海马TNF-α和IL-1β、HMGB-1 m-RNA表达亦明显上调，海马α7nAChR蛋白表达上调，海马CA1区肥大细胞活化数目、Tunel阳性细胞数目亦明显增加(P<0.05)。结论：电针可能通过抑制中枢肥大细胞活化，上调脑内α7nAChR蛋白表达，抑制TNF-α和IL-1β、HMGB-1表达和释放，进而改善胫骨骨折术后大鼠学习与记忆能力。 |
| 英文摘要: |
| ABSTRACT Objective: To observe the effects of electroacupuncture on cognitive function and hippocampal α7nAChR expression in rats with postoperative cognitive function. Methods: 120 male SD rats were selected and randomly divided into 5 groups: control group (Ctrl group), operation group (Op group), electroacupuncture group (EA), α7nAChR inhibitor (EA + α-BGT) group, α7nAChR agonist (PHA-543,613 group), 12 in each group. Each group was divided into two subgroups for 1 day and 3 days. Morris water maze was used to detect cognitive function, ELISA to detect serum TNF-α and IL-1β, HMGB-1 levels, RT-PCR to detect hippocampal TNF-α and IL-1β HMGB-1 m-RNA, Western blotting to detect hippocampal α7nAChR protein expression, toluidine blue method to detect hippocampal CA1 region mast cell activation number. Tunel method to detect cell apoptosis in hippocampal CA1 area. Results: Compared with the Op group, the EA group, PHA-543,613 group had significantly shorter escape latency on the 1st and 3rd day after surgery, and increased the number of platform crossings (P<0.05). Compared with the EA group, the EA+α-BGT group on the 1st and 3rd day after surgery, the escape latency was significantly extended, and the number of crossing platforms was significantly reduced (P<0.05). Compared with the Op group, the levels of serum TNF-α, IL-1β and HMGB-1 in the EA group, PHA-543,613 group decreased significantly on the 1st and 3rd day after operation, and the hippocampal TNF-α, IL-1β and HMGB-1 m-RNA expression was also significantly down-regulated, hippocampal α7nAChR protein expression was up-regulated, the number of activated mast cells in hippocampal CA1 area, and the number of Tunel-positive cells decreased. The difference between the two groups was statistically significant (P<0.05). Compared with the EA group, the levels of serum TNF-α, IL-1β and HMGB-1 in the EA +α-BGT group increased on the 1st and 3rd day after operation. The m-RNA expression of TNF-α, IL-1β and HMGB-1 in the hippocampus was also significantly up-regulated, the expression of α7nAChR protein in the hippocampus was up-regulated, and the number of activated mast cells and number of Tunel positive cells in the hippocampal CA1 area also increased significantly(P<0.05). Conclusion: Electroacupuncture may inhibit the activation of central mast cells, up-regulate the expression of α7nAChR protein in the brain, inhibit the expression and release of TNF-α, IL-1β, and HMGB-1, thereby improving the learning and memory ability of rats after tibial fracture. |
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