| ABSTRACT Objective: To explore the effect of miR-125a-5p transfection on the proliferation, invasion and migration of hepatoma cells. Methods: Liver cancer cells were divided into control group, down-regulation group and up-regulation group, and stable down-regulation group and up-regulation group were established by cell transfection. Cell proliferation was detected by MMT, apoptosis was detected by flow cytometry, cell invasion was detected by Transwell chamber test, cell migration was detected by cell scratch test, and protein expression of Akt, Bax, Bcl-2, P13K and p-Akt in P13K / Akt pathway was detected by Western blot. Results: Compared with the up-regulated group, the down-regulated group had significantly lower cell proliferation rates at 24, 48 and 72 h, cell invasion and cell migration, and the expression levels of AKT, bcl-2, P13K and p-akt were significantly lower in the down-regulated group (29.67±9.87 vs 17.34±5.71, t=5.192, P<0.05, 34.75±11.56 vs 15.17±5.04, t=7.365, P<0.05, 38.48±12.81 vs 12.51 ±4.13, t=9.153, P<0.05). 72.53±24.17 vs 36.28±12.07, t=6.365, P<0.05, 86.51±28.75 vs 46.28±15.32, t=5.858, P<0.05, 1.26±0.41 vs 0.81±0.26, t=4.397, P< 0.05, 1.35±0.44 vs 0.76±0.24, t=5.584, P<0.05, 1.48±0.46 vs 0.79±0.26, t=6.194, P<0.05, 1.22±0.39 vs 0.73±0.24, t=5.584, P<0.05); Compared with the up-regulated group, the apoptosis rate of 24, 48, and 72h cells in the down-regulated group was significantly increased, with statistically significant differences (17.62±5.84 vs 29.31±9.75, t=4.879, P<0.05, 14.97±4.65 vs 34.19±11.36, t=7.427, P<0.05, 11.26±3.74 vs 38.62±12.86, t=9.690, P<0.05, 0.75±0.24 vs 1.33±0.43, t=5.587, P<0.05). Conclusion: The down-regulation of mir-125a-5p can regulate the expression of Akt, Bax, Bcl-2, P13K and p-Akt protein by acting on P13K / Akt pathway, and then inhibit the proliferation, apoptosis, invasion and migration of hepatoma cells. |
