| 李晓静,黄刚莉,王 露,杜姿岑,张 霞.外周血白细胞介素-13与干扰素-γ在儿童特应性皮炎中的发病机制研究[J].,2020,(13):2533-2536 |
| 外周血白细胞介素-13与干扰素-γ在儿童特应性皮炎中的发病机制研究 |
| Study of Pathogenesis of Peripheral Blood Interleukin-13 and Interferon-γ in Children with Atopic Dermatitis |
| 投稿时间：2019-11-19 修订日期：2019-12-13 |
| DOI：10.13241/j.cnki.pmb.2020.13.028 |
| 中文关键词: 特应性皮炎 儿童 白细胞介素-13 干扰素-γ 免疫球蛋白E |
| 英文关键词: Atopic dermatitis Children Interleukin-13 Interferon-γ Immunoglobulin E |
| 基金项目:四川省卫生和计划生育委员会科研项目（16PJ1302） |
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| 中文摘要: |
| 摘要 目的：探讨外周血白细胞介素-13（IL-13）与干扰素-γ（IFN-γ）在儿童特应性皮炎（AD）发病机制中的作用。方法：选择2016年5月~2018年4月本院收治的AD患儿120例作为观察组，并根据欧洲特应性皮炎评分标准（SCORAD）将其分为轻度组（n=33）、中度组（n=53）、重度组（n=34），采用分层抽样的方法抽取同期在本院进行健康体检的120例健康儿童为对照组。采用酶联免疫吸附法（ELISA）检测外周血IL-13和IFN-γ水平，采用放射免疫法检测免疫球蛋白E（IgE）水平，比较各组IL-13、IFN-γ与IgE水平，采用Spearman相关分析IL-13、IFN-γ、IgE两两之间的相关性。结果：观察组儿童治疗前IL-13、IgE水平高于对照组体检时，IFN-γ水平低于对照组体检时，差异有统计学意义（P＜0.05）。治疗前IL-13与IgE呈正相关关系（r=0.762，P＜0.05），IFN-γ与IgE呈负相关关系（r=-0.673，P＜0.05），IL-13与IFN-γ呈负相关关系（r=-0.672，P＜0.05）。轻、中、重度组患儿治疗前的IL-13、IFN-γ、IgE水平整体比较差异有统计学意义（P<0.05），重度组、中度组治疗前IL-13、IgE水平高于轻度组，IFN-γ低于轻度组，且重度组治疗前IL-13、IgE水平高于中度组，IFN-γ低于中度组，差异均有统计学意义（P<0.05）。观察组治疗3、6个月后，IL-13水平和IgE水平明显低于治疗前，IFN-γ水平明显高于治疗前，差异有统计学意义（P＜0.05）。结论：AD患儿外周血IL-13水平升高，IFN-γ水平降低，二者可能通过影响IgE水平，引起辅助性T细胞1/辅助性T细胞2（Th1/Th2）失衡，从而参与AD的发病过程。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the pathogenesis of peripheral blood interleukin-13(IL-13) and interferon-γ(IFN-γ) in children with atopic dermatitis(AD). Methods: 120 children with AD who were treated in our hospital from May 2016 to April 2018 were selected as observation group, which were divided into mild group（n=33）, moderate group（n=53）, severe group（n=34） according to the European Scoring of Atopic Dermatitis(SCORAD), 120 healthy children for physical examination in our hospital during the same period were selected as control group by A stratified sampling method. The levels of peripheral blood IL-13 and IFN-γ were detected by the enzyme-linked immunosorbent assay(ELISA), and the level of immunoglobulin E(IgE) was detected by the radioimmunoassay. The correlations between IL-13, IFN-γ and IgE were analyzed by Spearman correlation analysis. Results: The levels of IL-13 and IgE in the observation group before treatment were higher than those in the control group when physical examination, while the levels of IFN-γ in the observation group was lower than that in the control group when physical examination, the differences were statistically significant(P<0.05). IL-13 was positively correlated with IgE before treatment(r=0.763, P<0.05), IFN-γ was negatively correlated with IgE before treatment(r=-0.673, P<0.05), and IL-13 was negatively correlated with IFN-γ before treatment(r=-0.672, P<0.05). The levels of IL-13, IFN-γ and IgE in mild, moderate and severe groups were significantly different before treatment(P<0.05), the levels of IL-13 and IgE in severe group and moderate group were higher than those in mild group, while the levels of IFN-γ was lower than that in mild group before treatment, and the levels of IL-13 and IgE in severe group were higher than those in moderate group, and the levels of IFN-γ was lower than that in moderate group before treatment, the differences were statistically significant(P<0.05). The levels of IL-13 and IgE in observation group at 3 and 6 months after treatment were lower than those before treatment, while IFN-γ was higher than that before treatment, the differences were statistically significant(P<0.05). Conclusion: The levels of IL-13 in peripheral blood of children with AD increase, while the levels of IFN-γ decrease, which may cause imbalance of helper T cell 1/helper T cell 2 (Th1/Th2) by affecting IgE level, thus participating in the pathogenesis of AD. |
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