| 拜云虎,吴菲菲,孔二亮,王亚云,王全晖,杨雁灵.急性肝损伤时肝脏内Pink1/Parkin的表达变化及意义[J].,2020,(9):1648-1651 |
| 急性肝损伤时肝脏内Pink1/Parkin的表达变化及意义 |
| Expression of Pink1/Parkin in Liver during Acute Liver Injury and Its Significance |
| 投稿时间：2019-10-03 修订日期：2019-10-28 |
| DOI：10.13241/j.cnki.pmb.2020.09.009 |
| 中文关键词: 急性肝损伤 肝脏 Pink1 Parkin |
| 英文关键词: Acute liver injury Liver Pink1 Parkin |
| 基金项目:国家自然科学基金项目（81470843） |
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| 中文摘要: |
| 摘要 目的：观察C57小鼠急性肝损伤（Acute liver injury, ALI）中线粒体自噬相关蛋白Pink1/Parkin表达的变化及意义。方法：领取28只小鼠，随机分为对照组、ALI 1 d组、ALI 4 d组、ALI 7 d组。分别检测血清谷氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）的变化；肝组织病理变化用H&E染色观察；Real Time quality PCR检测Pink1/Parkin在mRNA水平的表达变化；进一步利用Western Blot方法分别检测肝组织中Pink1/Parkin蛋白水平的表达变化。结果：ALI组1 d、4 d、7 d组与对照组相比，ALT、AST表达显著升高，具有统计学意义（P<0.05）；肝组织病理学检查ALI组1 d和4 d组中，释放出大量的炎性细胞，肝细胞出现大面积坏死，7 d组肝细胞坏死与1 d和4 d比较明显缓解；mRNA水平检测Pink1/Parkin在ALI 1 d（4.79/1.82倍）、4 d（1.74/4.17倍）和7 d（2.91/1.25倍）与对照组相比表达水平明显升高（P<0.05）；Pink1/Parkin在蛋白水平检测时ALI 1 d（2.03/1.82倍）、4 d（1.78/4.17倍）和7 d（1.25/1.25倍）时肝组织内表达也显著增加，差异具有统计学意义（P<0.05）。结论：急性肝损伤状态下Pink1/Parkin在mRNA和蛋白水平表达升高，且其表达量随着肝脏的损伤缓解而表达水平降低，提示Pink1/Parkin可能对于治疗急性肝损伤具有重要意义。 |
| 英文摘要: |
| ABSTRACT Objective: To observe the change of mitochondrial autophagy-associated protein Pink1/Parkin expression in acute liver injury (ALI) of C57 mice and its significance. Methods: Twenty-eight mice were randomly divided into control group, ALI 1 d group, ALI 4 d group, and ALI 7 d group. The change of serum glutamate aminotransferase (ALT) and aspartate aminotransferase (AST) were detected respectively. The pathological changes of liver tissue were observed by H&E staining. The expression of Pink1/Parkin in mRNA level were detected by Real Time quality PCR. Next step, the expression of Pink1/Parkin in protein level of liver tissue were detected by the Western blot methods. Results: The level of ALT and AST in ALI groups at 1 d, 4 d and 7 d were significantly higher than in the sham group (P<0.05). H&E staining showed that a mass of inflammatory cells infiltration and cells degeneration were observed in ALI groups, and hepatocyte necrosis in 7 d group were significantly relieved than 1 d and 4 d. The result of real time quality-PCR indicated that the level of Pink1/Parkin enhanced significantly at 1 d (4.79/1.82 fold), 4 d (1.74/4.17 fold) and 7 d (2.91/1.25 fold) in ALI groups compared with sham group (P<0.05). Western blot results showed that the expression of Pink1/Parkin were detected in the extracted protein of liver tissue which were significantly increased in the group of ALI 1 d (2.03/1.82 fold), 4 d (1.78/4.17 fold) and 7 d (1.25/1.25 fold）compared with sham group (P<0.05). Conclusion: Pink1 and Parkin were highly expression in protein and mRNA levels of acute liver injury, and its expression level were decreased with the relief of liver tissue damage. In addition, Pink1/Parkin could be have a potential significance for the treatment of acute liver injury. |
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