陈怡环,薛姗姗,顾婷婷,王化宁,彭正午.电针早期干预对创伤后应激模型大鼠的行为及前额叶皮质BDNF、IL-1β和IL-6水平的影响[J].,2020,(9):1619-1623 |
电针早期干预对创伤后应激模型大鼠的行为及前额叶皮质BDNF、IL-1β和IL-6水平的影响 |
Effect of Early Intervention with Electroacupuncture on Anxiety-like Behavior and Expression of BDNF, IL-1β and IL-6 in the Prefrontal Cortex of PTSD Rats Model |
投稿时间:2019-10-28 修订日期:2019-11-24 |
DOI:10.13241/j.cnki.pmb.2020.09.004 |
中文关键词: 电针 脑源性神经营养因子 白细胞介素-6 创伤后应激障碍 |
英文关键词: Electroacupuncture Brain-derived neurotrophic factor Interleukin-6 Posttraumatic stress disorder |
基金项目:国家自然科学基金项目(81571309) |
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中文摘要: |
摘要 目的:观察电针(Electroacupuncture,EA)早期干预对创伤后应激(Posttraumatic stress disorder,PTSD)模型大鼠的焦虑样行为及前额叶皮质(Prefrorntal cortex,PFC)中脑源性神经营养因子(Brain-derived neurotrophic factor,BDNF)、白介素1β(Interleukin-1beta,IL-1β)和白介素6(Interleukin-6,IL-6)水平的影响。方法:将32只雄性SD大鼠经环境适应后,随机分为Sham组、Sham + EA组、ESPS组和ESPS + EA组,每组8只。对ESPS组和ESPS + EA组大鼠进行增强型单次延长应激(Enhanced single prolonged stress,ESPS)造模处理,其他两组不接受ESPS,但是置于同一实验室环境。造模结束后24 h,各组大鼠进行EA干预:Sham + EA组和ESPS + EA组的大鼠每天接受EA刺激(百会穴,1 mA,2/15 Hz)30 min,连续1周;另外两组给予假刺激(无电流)每天30 min,连续1周。静置一周后,采用旷场和高架十字实验观察各组大鼠的行为,之后处死大鼠,分别用蛋白质印迹法和酶联免疫法(Enzyme-linked immunosorbent assay,ELISA)检测各组大鼠PFC中BDNF的表达水平以及IL-1β和IL-6的水平。结果:(1)ESPS处理导致大鼠焦虑样行为,在旷场中心区运动距离和探索时间百分比减少,在高架十字开臂运动距离及停留时间百分比减少。ESPS组大鼠PFC中BDNF表达下降,IL-1β和IL-6的水平升高;(2)EA早期干预可以改善大鼠的焦虑样行为,提高ESPS模型大鼠PFC中BDNF的表达,降低IL-6的水平,对IL-1β的影响无统计学差异。结论:EA早期干预改善了ESPS诱导的大鼠焦虑样的行为,这可能与其增加了PFC中BDNF表达,降低了炎性因子IL-6的表达有关。 |
英文摘要: |
ABSTRACT Objective: To investigate the effect of early intervention with Electroacupuncture (EA) on the anxiety-like behavior of ESPS-treated rat and the regulation of BDNF, IL-1β and IL-6 in the prefrontal cortex (PFC). Methods: Thirty - two male Sprague - Dawley rats were randomly divided into Sham, Sham + EA, ESPS and ESPS + EA group after adjusting the environment for 1 week. Rats in ESPS group and ESPS + EA group were exposed to ESPS, and rats in Sham and Sham + EA groups were placed in the same experimental environment but did not receive ESPS at the same time. 24 h later, rats in Sham + EA group and ESPS + EA group received EA treatment (Baihui, 1 mA, 2/15 Hz) and the other two groups received sham stimulation (acupuncture treatment without electricity) for 30 min every day for 1 week. The open field test and elevated-plus maze test were performed 1 week later. Then all rats were sacrificed and the level of BDNF and the level of IL-1β and IL-6 in PFC were measured by Western blot and ELISA, respectively. Results: (1) The traveled distance and the percentage of exploration time in central region of open field test and the percentage of exploration time in open arms of elevated-plus maze test were significantly decreased in ESPS group than that of other groups. The expression of BDNF in PFC was decreased, while the levels of IL-1β and IL-6 were increased in the ESPS group than that of other groups. (2) Early intervention of EA ameliorated the anxiety-like behavior of rats, elevated the expression of BDNF and reduced the level of IL-6 in PFC of ESPS-treated rats. However, there was no significant different between ESPS group and ESPS + EA group on the level of IL-1β. Conclusion: Early intervention with EA improved ESPS-induced anxiety-like behavior in rats, which might be related to the increase of BDNF expression and decrease of the level of IL-6 in PFC. |
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