| 李 会,王 玎,卢 娇,徐 珍,刘 涛.宫颈癌组织RACK-1、Lgr5表达与临床病理特征及预后的关系[J].,2020,(8):1540-1544 |
| 宫颈癌组织RACK-1、Lgr5表达与临床病理特征及预后的关系 |
| Relationship between Expression of RACK-1 and Lgr5 and Clinicopathological Features and Prognosis in Cervical Cancer Tissues |
| 投稿时间：2019-08-27 修订日期：2019-09-22 |
| DOI：10.13241/j.cnki.pmb.2020.08.031 |
| 中文关键词: 宫颈癌 RACK-1 Lgr5 临床病理特征 预后 |
| 英文关键词: Cervical cancer RACK-1 Lgr5 Clinicopathological features Prognosis |
| 基金项目:湖北省教育厅科学研究计划重点项目（D20151202） |
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| 中文摘要: |
| 摘要 目的：探讨宫颈癌（CC）组织激活的蛋白激酶C受体1（RACK-1）、富含亮氨酸重复序列的G蛋白偶联受体5（Lgr5）表达与临床病理特征及预后的关系。方法：选取2012年1月-2016年1月于三峡大学附属仁和医院接受手术治疗的134例CC患者作为研究对象，选取CC组织以及对应的癌旁正常组织，同时选取该院因宫颈良性病变行肿物剥除或附件切除的134例患者的正常宫颈组织作为对照，采用免疫组化链霉素抗生物素蛋白－过氧化物酶连（SP）法对各组织进行免疫组化染色，依据阳性细胞率和染色强度分析CC组织中RACK-1、Lgr5的表达情况，并分析RACK-1、Lgr5表达与CC患者临床病理特征的相关性，分析Lgr5及RACK-1不同阳性表达强度的CC患者的预后。结果：CC组织中RACK-1、Lgr5阳性表达率高于癌旁组织和正常宫颈组织，差异有统计学意义（P<0.05)；RACK-1、Lgr5阳性表达与TNM分期、肿瘤分化程度、淋巴结转移、脉管内癌栓相关（P<0.05)，与患者的年龄、肿瘤大小、病理类型、肿瘤浸润深度、宫旁侵犯、手术切缘无关（P>0.05）；Lgr5、RACK-1阳性高表达组患者的3年生存率、生存时间低于Lgr5、RACK-1阳性低表达组患者，差异有统计学意义（P<0.05）。结论：RACK-1、Lgr5在CC组织中表达上调，并与部分临床病理特征及预后有关，检测RACK-1、Lgr5有助于CC患者的诊断及预后评估。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the relationship between the expression of receptor for activated C kinase 1 (RACK-1) and leucine-rich repeat-containing G protein-coupled receptor 5 (Lgr5) and clinicopathological features and prognosis in cervical cancer tissues (CC). Methods: 134 patients with CC who underwent surgical treatment in Affiliated Renhe Hospital of China Three Gorges University from January 2012 to January 2016 were selected as the study subjects. CC tissues and corresponding normal tissues adjacent to cancer were selected, and normal cervical tissues of 134 patients with benign lesions or attachment resection of benign lesions in the hospital as a control. Immunohistochemical streptomycin antibiotic protein-peroxidase linkage (SP) was used to stain various tissues. The expression of RACK-1 and Lgr5 in CC tissues was analyzed according to the positive cell rate and staining intensity, and the correlation between the expression of RACK-1 and Lgr5 and the clinicopathological characteristics of patients with CC was analyzed. Prognosis of patients with CC with different positive expression intensity of Lgr5 and RACK-1. Results: The positive expression rates of RACK-1 and Lgr5 protein in cervical cancer tissues were higher than those in adjacent tissues and normal cervical tissues, the differences were statistically significant (P<0.05). The positive expression of RACK-1 and Lgr5 were closely related to TNM stage, tumor differentiation, lymph node metastasis, intravascular tumor thrombus (P<0.05), and they were not with the age, tumor size, pathological type, depth of tumor invasion, parametrial invasion, surgery cutting edge (P>0.05). The 3-year survival rate and survival time of patients with positive high expression of Lgr5 and RACK-1 were lower than those of patients with positive low expression of Lgr5 and RACK-1, the differences were statistically significant (P<0.05). Conclusion: The expression of RACK-1 and Lgr5 are up-regulated in CC tissues, which are related to some clinicopathological features and prognosis. Detection of RACK-1 and Lgr5 is helpful to the diagnosis and prognosis of patients with CC. |
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