| 杨莹莹,张 坤,毛康琳,孙 靓,穆 欣.环状RNA Circ_0001073在恶性黑素瘤中的表达及其对A375细胞恶性表型的影响[J].,2020,(7):1236-1240 |
| 环状RNA Circ_0001073在恶性黑素瘤中的表达及其对A375细胞恶性表型的影响 |
| Expression of Circular RNA Circ_0001073 in Malignant Melanoma and Its Effects on Malignant Phenotypes of A375 Cells |
| 投稿时间：2019-09-30 修订日期：2019-10-25 |
| DOI：10.13241/j.cnki.pmb.2020.07.007 |
| 中文关键词: 恶性黑素瘤 Circ_0001073 增殖 迁移 侵袭 |
| 英文关键词: Malignant melanoma Circ_0001073 Proliferation Migration Invasion |
| 基金项目:国家自然科学基金项目（81802935）；陕西省自然科学基金项目（2019JM-487） |
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| 中文摘要: |
| 摘要 目的：通过检测环状RNA Circ_0001073在恶性黑素瘤细胞与正常表皮黑素细胞中的表达差异，及其对恶性黑素瘤A375细胞恶性表型的影响，阐明Circ_001073在恶性黑素瘤细胞中的表达及功能。方法：采用聚合酶链式反应（Real-time quantitative polymerase chain reaction, RT-QPCR）方法检测正常表皮黑素细胞和恶性黑素瘤细胞中Circ_0001073的表达水平；应用小干扰RNAs沉默恶性黑素瘤A375细胞中Circ_0001073表达，5-乙炔基-2'-脱氧尿苷（5-Ethynyl-2'-deoxyuridine，EdU）实验检测沉默Circ_0001073对A375细胞增殖的影响，平板克隆实验检测沉默Circ_0001073对A375细胞克隆形成的影响，细胞划痕实验评估沉默Circ_0001073对A375细胞迁移能力的影响，Transwell实验评估沉默Circ_0001073对A375细胞侵袭能力的影响。结果：qRT-PCR结果显示Circ_0001073在恶性黑素瘤细胞中的表达低于正常表皮黑素HEMa-LP细胞（P<0.05）。应用Circ_0001073 siRNA转染A375细胞后，Circ_0001073的表达水平较转染Circ_0001073 NC的细胞显著降低（P<0.05）。EdU实验结果显示：沉默A375细胞中Circ_0001073的表达后，A375细胞增殖能力显著增强（P<0.05）；平板克隆实验结果显示：沉默A375细胞中Circ_0001073的表达后，A375细胞克隆形成能力显著增强（P<0.05）；细胞划痕实验结果显示：沉默A375细胞中Circ_0001073的表达后，细胞迁移能力显著增强（P<0.05）；Transwell实验结果显示：沉默A375细胞中Circ_0001073的表达后，细胞侵袭能力显著增强（P<0.05）。结论：Circ_0001073在恶性黑素瘤细胞中低表达，并可抑制A375细胞的增殖、克隆形成、迁移及侵袭。 |
| 英文摘要: |
| ABSTRACT Objective: To demonstrate the expression and function of Circ_0001073 in A375 cells by investigating the expression of circular RNA Circ_0001073 in malignant melanoma cells and normal epidermal melanoma cells, and exploring the effects of Circ_0001073 on malignant phenotypes of A375 cells. Methods: Real-time quantitative polymerase chain reaction (RT-QPCR) was performed to detect the expression of Circ_0001073 in normal epidermal melanoma cells and malignant melanoma cells. Silencing the expression of Circ_0001073 in A375 cells by small interfering RNAs (siRNAs). 5-Ethynyl-2'-deoxyuridine (EdU) assays were performed to detect the effects of Circ_0001073 knockdown on cell proliferation of A375 cells; Colony forming assays were performed to detect the effects of Circ_0001073 knockdown on colony forming ability of A375 cells; Wound healing assays were perform to detect the effects of Circ_0001073 knockdown on migrative ability of A375 cells; Transwell assays were performed to detect the effects of Circ_0001073 knockdown on invasive ability of A375 cells. Results: Compared to normal epidermal melanocytes HEMa-LP cells, Circ_0001073 was downregulated in malignant melanoma cells(P<0.05). After silencing the expression of Circ_0001073 by siRNAs in A375 cells, the expression of Circ_0001073 was significantly down-regulated in A375 cells transfected with Circ_0001073 siRNA compared to A375 cells transfected with Circ_0001073 NC(P<0.05). EdU assays indicated the cell proliferation was enhanced after silencing the expression of Circ_0001073 in A375 cells(P<0.05); Colony forming assays indicated the colony forming ability was enhanced after silencing the expression of Circ_0001073 in A375 cells (P<0.05); Wound healing assays indicated the cell migrative ability was enhanced after silencing the expression of Circ_0001073 in A375 cells (P<0.05); Transwell assays indicated the cell invasive ability was enhanced after silencing the expression of Circ_0001073 in A375 cells(P<0.05). Conclusion: Circ_0001073 is down-regulated in malignant melanoma cells, and Circ_0001073 could inhibits cell proliferation, colony forming, migrative and invasive ability of A375 cells. |
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