| 马 钰,韩 琳,李 冰,王淑艳,沈一凡,贾祎凡,杨 柳,秦建国.降压通络方对大鼠肾小管上皮细胞凋亡的影响[J].,2020,(7):1211-1216 |
| 降压通络方对大鼠肾小管上皮细胞凋亡的影响 |
| Effect of JiangyaTongluo Formula on Apoptosis of Rat Renal Tubular Epithelial Cells |
| 投稿时间：2019-12-07 修订日期：2019-12-31 |
| DOI：10.13241/j.cnki.pmb.2020.07.003 |
| 中文关键词: 高血压肾损害 降压通络方 肾小管上皮细胞 缺血缺氧 细胞凋亡 |
| 英文关键词: Hypertensive Nephrosclerosis JiangyaTongluo Formula Renal tubular epithelial cells Ischemia-hypoxia Cell apoptosis |
| 基金项目:国家自然科学基金项目（811734071）；北京市中医管理局北京中医药"薪火传承3+3工程"建设项目郭士魁名家研究室项目（2011-SZ-A-27） |
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| 中文摘要: |
| 摘要 目的：研究降压通络方对大鼠肾小管上皮细胞凋亡的影响。方法：体外培养大鼠肾小管上皮细胞，经过缺血缺氧，将大鼠肾小管细胞随机分为4组：正常组、模型组、降压通络方组、缬沙坦组。采用CCK-8检测细胞增殖情况，免疫组化法检测大鼠肾小管上皮细胞p-AKT蛋白的表达，蛋白免疫印迹法检测大鼠肾小管上皮细胞凋亡相关基因调控蛋白Bcl-2、Bax的表达，对Bcl-2、Bax蛋白的表达水平进行相关性分析。结果：缺血缺氧呈时间依赖性抑制大鼠肾小管上皮细胞活性；免疫组化结果示：p-AKT在正常组呈高表达，模型组低表达，降压通络方组p-AKT表达明显高于模型组（P＜0.01），缬沙坦组表达低于模型组（P＞0.05）；蛋白免疫印迹法结果示：模型组Bcl-2表达较正常组明显降低（P＜0.01），降压通络方组和缬沙坦组Bcl-2的表达均高于模型组（P＜0.05，P＜0.01）；模型组Bax表达较正常组明显升高（P＜0.01），降压通络方组和缬沙坦组Bax的表达均较模型组降低（P＜0.05）；Bcl-2蛋白与Bax蛋白表达呈负相关性（r=-0.811，P＜0.01）。结论：降压通络方可抑制大鼠肾小管上皮细胞凋亡，其作用机制可能与上调p-AKT、Bcl-2蛋白，下调Bax蛋白表达有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of JiangyaTongluo Formula on apoptosis of renal tubular epithelial cells in rats. Methods: Rat renal tubular epithelial cells were cultured in vitro and then randomly divided into 4 groups after ischemia and hypoxia: normal group, model group, JiangyaTongluo Formula group, valsartan group. Cell proliferation was detected by CCK-8, p-AKT protein expression in rat renal tubular epithelial cells was detected by immunocytochemistry, and expression of apoptosis-related gene regulatory proteins Bcl-2 and Bax in rat renal tubular epithelial cells was detected by Western blot. Correlation analysis was conducted on the expression levels of Bcl-2 and Bax proteins. Results: Ischemia and hypoxia inhibited the activity of rat renal tubular epithelial cells in a time-dependent manner; Immunocytochemical results showed that the expression of p-AKT was high in the normal group, but low in the model group. The expression of p-AKT in JiangyaTongluo Formula group was significantly higher than that in the model group (P<0.01), and that in the valsartan group was lower than that in the model group (P>0.05); Western blot results showed that the expression of Bcl-2 in the model group was significantly lower than that in the normal group (P<0.01), while the expression of Bcl-2 in JiangyaTongluo Formula group and the valsartan group was higher than that in the model group (P<0.05, P<0.01). Bax expression in the model group was significantly higher than that in the normal group(P<0.01), while Bax expression in JiangyaTongluo Formula group and the valsartan group was significantly lower than that in the model group (P<0.05); the expression of Bcl-2 protein and Bax protein was negatively correlated（r=-0.811, P<0.01）. Conclusion: The apoptosis of rat renal tubular epithelial cells can be inhibited by JiangyaTongluo Formula, and the mechanism may be related to the up-regulation of p-AKT and Bcl-2 proteins and the down-regulation of Bax protein expression. |
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