| 周小明,邓桂明,龙 云,刘建和,吴亦之,谭 琦.CXCL8对小鼠动脉粥样硬化发生发展的影响及其分子机制研究[J].,2020,(6):1057-1060 |
| CXCL8对小鼠动脉粥样硬化发生发展的影响及其分子机制研究 |
| Effects of CXCL8 on the Development of Atherosclerosis in Mice and Its Molecular Mechanism Study |
| 投稿时间：2019-11-23 修订日期：2019-12-18 |
| DOI：10.13241/j.cnki.pmb.2020.06.012 |
| 中文关键词: 动脉粥样硬化 趋化因子8 基质金属蛋白酶9 血脂 发病机制 |
| 英文关键词: Atherosclerosis Mhemokinefactor8 Matrix metalloproteinase9 Blood lipid Pathogenesis |
| 基金项目:教育部重点实验室开放基金项目（ZYNK201702） |
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| 中文摘要: |
| 摘要 目的：探讨趋化因子8(CXCL8)对小鼠动脉粥样硬化(AS)发生发展的影响及其可能机制。方法：40只雄性Apo E-/-小鼠随机分为AS组和CXCL8抑制剂（G31P）组，每组各20只，另选取20只雄性C57BL/6J小鼠作为对照组。AS组小鼠予高脂饲料喂养12周，G31P组小鼠予高脂饲料喂养12周的同时皮下注射G31P（注射剂量为500 μg/kg），对照组的小鼠予普通饲料喂养12周。ELISA法检测小鼠血清中的甘油三脂(TG)、低密度脂蛋胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、总胆固醇(TC)水平，实时聚合酶链反应(RT-PCR)法检测小鼠主动脉组织中CXCL8及基质金属蛋白酶9(MMP9)mRNA的表达量，Western-blot法检测主动脉CXCL8及MMP9蛋白表达量。结果：高脂饮食喂养12周后，AS组小鼠血清中的TG、LDL-C、TC水平均高于G31P组，而HDL-C水平明显低于G31P组(P<0.05)；G31P组小鼠血清中的TG、LDL-C、TC水平均高于对照组，HDL-C水平低于对照组(P<0.05)。AS组小鼠主动脉组织中的CXCL8、MMP9的mRNA表达量及蛋白表达量高于G31P组(P<0.05)，G31P组小鼠主动脉组织中的的CXCL8、MMP9的mRNA表达量及蛋白表达量高于对照组(P<0.05)。结论：CXCL8在AS小鼠主动脉组织中呈高表达，CXCL8可能通过影响MMP9的表达在一定程度上参与了AS的发生发展，抑制CXCL8的表达或可延缓AS的发生发展。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of chemokine 8(CXCL8) on the development of atherosclerosis (AS) in mice and its possible mechanism. Methods: 40 male Apo E-/- mice were randomly divided into AS group and G31P group, 20 mice in each group, meanwhile 20 male C57BL/6J mice was assigned into the control group. AS group mice were fed with high fat diet for 12 weeks, G31P group mice were were fed with high fat diet for 12 weeks and subcutaneous injection at the same time of G31P(The injection volume is 500 μg/kg), The control group mice were fed with regular diet for 12 weeks. Then ELISA method was used to measure serum triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), total cholesterol (TC) level. Real-time polymerase chain reaction (RT-PCR) were used to measure CXCL8mRNA and MMP9mRNA expression in aorta tissue. Western-blot methods was performed to test the protein expression levels of CXCL8 and MMP9 in aorta tissue. Results: After 12 weeks high-fat diet feeding, the levels of TG, LDL-C and TC in serum of AS group were higher than those in the G31P group, while the HDL-C level was lower than that in the G31P group (P<0.05). The levels of TG, LDL-C and TC in the serum of G31P group were higher than control group, HDL-C level was lower than the control group(P<0.05). The expression levels of CXCL8mRNA, MMP9mRNA, CXCL8 protein and MMP9 protein in aortic tissue of AS group were higher than those in G31P group (P<0.05), and the expression levels of CXCL8mRNA, MMP9mRNA, CXCL8 protein and MMP9 protein in aortic tissue of G31P group were higher than those in the control group(P<0.05). Conclusion: CXCL8 has high expression in the aortic tissues of as mice, it may be involved in the development of as by affecting the expression of MMP9, inhibiting the expression of CXCL8 or delaying the development of AS. |
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