| 李 莉,孙颖颖,王怡璐,魏庆庆,王 冀.miR-125b在急性加重期慢性阻塞性肺疾病患者血浆中的表达及与肺功能和炎症细胞因子的关系[J].,2020,(5):944-948 |
| miR-125b在急性加重期慢性阻塞性肺疾病患者血浆中的表达及与肺功能和炎症细胞因子的关系 |
| Expression of miR-125b in Plasma of Patients with Acute Exacerbation of Chronic Obstructive Pulmonary Disease and Its Relationship with Pulmonary Function and Inflammatory Cytokines |
| 投稿时间：2019-05-29 修订日期：2019-06-23 |
| DOI：10.13241/j.cnki.pmb.2020.05.032 |
| 中文关键词: 微小RNA-125b 慢性阻塞性肺疾病 肺功能 炎症细胞因子 相关性 |
| 英文关键词: MicroRNA-125b Chronic obstructive pulmonary disease Pulmonary function Inflammatory cytokines Relevance |
| 基金项目:国家自然科学基金青年科学基金项目（81600195） |
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| 中文摘要: |
| 摘要 目的：探讨微小RNA-125b（miR-125b）在急性加重期慢性阻塞性肺疾病（COPD）患者血浆中的表达及其与肺功能、炎症细胞因子的关系。方法：选择2016年11月至2019年1月应急总医院收治的69例急性加重期COPD患者作为急性加重组，并于同期随机选取58例稳定期COPD患者作为稳定组和50例健康体检者作为对照组。采用实时荧光定量PCR法检测各组血浆miR-125b表达水平；采用肺功能检测仪测定肺功能，包括用力肺活量（FVC）、第1秒用力呼气量（FEV1）、FEV1/FVC；采用酶联免疫吸附法测定血清炎症细胞因子，包括白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、高敏C反应蛋白（hs-CRP）、肿瘤坏死因子-α（TNF-α）。结果：急性加重组血浆miR-125b表达水平高于稳定组和对照组，差异有统计学意义（P<0.05）。急性加重组、稳定组肺功能指标FVC、FEV1、FEV1/FVC低于对照组，且急性加重组低于稳定组，差异有统计学意义（P<0.05）。急性加重组、稳定组血清炎症细胞因子IL-6、IL-8、hs-CRP、TNF-α水平高于对照组，且急性加重组高于稳定组，差异有统计学意义（P<0.05）。Pearson相关分析结果显示，急性加重期COPD患者血浆miR-125表达水平与FVC、FEV1、FEV1/FVC呈负相关（P<0.05），与IL-6、IL-8、hs-CRP、TNF-α呈正相关（P<0.05）。结论：miR-125b在急性加重期COPD患者血浆中异常表达，并与肺功能及炎症细胞因子密切相关，可作为临床辅助诊断及评估患者病情严重程度的参考指标。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the expression of microRNA-125b (miR-125b) in plasma of patients with acute exacerbation of chronic obstructive pulmonary disease (COPD) and its relationship with pulmonary function and inflammatory cytokines. Methods: 69 patients with acute exacerbation of COPD (acute exacerbation group) admitted to Emergency General Hospital from November 2016 to January 2019 were selected. At the same time, a total of 58 stable COPD patients (stable group) and 50 healthy volunteers (control group) were randomly selected. The expression level of plasma miR-125b were detected by real-time fluorescence quantitative PCR method. The pulmonary function was measured using a pulmonary function tester, including forced vital capacity (FVC), forced expiratory volume in one second (FEV1), FEV1/FVC. The serum inflammatory factors were tested by enzyme-linked immunosorbent assay, including interleukin-6 (IL-6), interleukin-8 (IL-8), high-sensitive C-reactive protein (hs-CRP), tumor necrosis factor-α (TNF-α). Results: The expression level of miR-125b in acute exacerbation group was higher than that in stable group and control group, the difference was statistically significant (P<0.05). The FVC, FEV1, FEV1/FVC in acute exacerbation group and stable group were lower than those in control group, and the acute exacerbation group were higher than those in stable group, the difference was statistically significant (P<0.05). The levels of IL-6, IL-8, hs-CRP, TNF-α in acute exacerbation group and stable group were higher than those in control group, and the acute exacerbation group were higher than those in stable group, the difference was statistically significant (P<0.05). Pearson correlation analysis results showed that the expression level of plasma miR-125b in patients with COPD in acute exacerbation stage was negatively correlated with FVC, FEV1 and FEV1/FVC (P<0.05), and was positively correlated with IL-6, IL-8, hs-CRP, TNF-α (P<0.05). Conclusion: The plasma miR-125b in patients with COPD in acute exacerbation stage is abnormally expressed, and it is closely correlated with pulmonary function and inflammatory factors, which can be used as an important index for clinical diagnosis and evaluation of the severity of the disease. |
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