| 杨 敬,代俞燕,熊燕影,任 静,骆 言.人参皂苷Rg1对糖尿病肾病大鼠血清氧化应激指标、炎性因子及肾组织TGF-β1、MCP-1 mRNA的影响[J].,2020,(5):853-856 |
| 人参皂苷Rg1对糖尿病肾病大鼠血清氧化应激指标、炎性因子及肾组织TGF-β1、MCP-1 mRNA的影响 |
| Effects of Ginsenoside Rg1 on Serum Oxidative Stress Indicators, Inflammatory Factors and Kidney Tissue TGF-β1 and MCP-1 mRNA in Diabetic Nephropathy Rats |
| 投稿时间：2019-09-05 修订日期：2019-09-28 |
| DOI：10.13241/j.cnki.pmb.2020.05.011 |
| 中文关键词: 糖尿病肾病 人参皂苷Rg1 氧化应激 炎性因子 单核细胞趋化因子蛋白-1 生长转化因子-β1 |
| 英文关键词: Diabetic nephropathy Ginsenoside Rg1 Oxidative stress Inflammatory factors Monocyte chemokine protein-1 Issue transforming growth factor-β1 |
| 基金项目:重庆市卫生计生委中医药科技项目（ZY201601102） |
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| 中文摘要: |
| 摘要 目的：研究人参皂苷Rg1对糖尿病肾病（DN）大鼠血清氧化应激指标、炎性因子及肾组织生长转化因子-β1（TGF-β1）、单核细胞趋化因子蛋白-1（MCP-1） mRNA的影响。方法：选取60只SD大鼠，将其以随机抽签法分为Rg1组、模型组以及对照组，各20只。其中Rg1组与模型组大鼠均选择腹腔内一次性注射链脲佐菌素（STZ）55 mg/kg建立DN大鼠模型，Rg1组予以人参皂苷Rg1治疗，模型组与对照组则予以等量的生理盐水干预。比较干预12周后各组肾功能相关指标、血清氧化应激指标、炎性因子及肾组织TGF-β1、MCP-1 mRNA表达情况。结果：Rg1组、模型组大鼠干预12周后血肌酐（Scr）、尿素氮（BUN）、胱抑素C（CysC）水平均高于对照组，而Rg1组大鼠干预12周后Scr、BUN、CysC水平均低于模型组（均P＜0.05）。Rg1组、模型组大鼠干预12周后血清超氧化物歧化酶（SOD）、谷胱甘肽（GSH）水平均低于对照组，丙二醛（MDA）水平高于对照组（均P＜0.05）；Rg1组大鼠干预12周后血清SOD、GSH水平均高于模型组，MDA水平低于模型组（均P＜0.05）。Rg1组、模型组大鼠干预12周后血清白细胞介素-6（IL-6）、白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）水平均高于对照组，而Rg1组血清炎性因子水平低于模型组（均P＜0.05）。Rg1组、模型组大鼠干预12周后肾组织TGF-β1、MCP-1 mRNA表达水平均高于对照组，且Rg1组低于模型组（均P＜0.05）。结论：人参皂苷Rg1可显著改善DN大鼠血清氧化应激指标，下调血清炎性因子水平以及肾组织TGF-β1、MCP-1 mRNA表达。 |
| 英文摘要: |
| ABSTRACT Objective: To study the effects of ginsenoside Rg1 on serum oxidative stress indicators, inflammatory factors, kidney tissue transforming growth factor-β1 (TGF-β1) and monocyte chemoattractant protein-1 (MCP-1) mRNA of diabetic nephropathy (DN) rats. Methods: 60 SD rats were selected as, which were divided into Rg1 group, model group and control group according to random lottery method, 20 SD rats in each group. Rats in the Rg1 group and model group were given one-time intraperitoneal injection of streptozotocin (STZ) 55 mg/kg to establish DN rat model. Rg1 group was given Rg1 intervention, model group and control group were given the same amount of normal saline intervention. Renal function related indicators, serum oxidative stress indicators, inflammatory factors and mRNA expressions of kidney tissue TGF-β1 and MCP-1 were compared 12 weeks after intervention. Results: 12 weeks after intervention, the serum creatinine(Scr), Blood urea nitrogen(BUN) and Cystatin C(CysC) levels of rats in the Rg1 group and model group were higher than those in the model group, while The levels of Scr, BUN and CysC in Rg1 group were lower than those in model group at 12 weeks after intervention (all P<0.05). 12 weeks after intervention, the serum superoxide dismutase(SOD) and glutathione(GSH) levels of rats in Rg1 group and model group were lower than those in control group, while malondialdehyde (MDA) level was higher than those in control group (all P<0.05). The serum SOD and GSH levels of rats in the Rg1 group were higher than those in the model group, and MDA level was lower than those in the model group at 12 weeks after intervention (all P<0.05). Serum interleukin-6(IL-6), interleukin-1β(IL-1β), and tumor necrosis factor-α(TNF-α) of rats in the Rg1 group and the model group were all higher than those in the control group at 12 weeks after intervention, while the serum inflammatory factors levels in the Rg1 group were lower than those in the model group (all P<0.05). The expression levels of kidney tissue TGF-β1mRNA and MCP-1 mRNA of rats in the Rg1 group and model group were higher than those in the control group at 12 weeks after intervention, which in the Rg1 group were lower than those in the model group (all P<0.05). Conclusion: Ginsenoside Rg1 can significantly improve the serum oxidative stress indicators of DN rats, down-regulate the expression level of inflammatory factors and mRNA expressions of kidney tissue TGF-β1and MCP-1. |
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