| 林智斌,汪建林,刘 奇,程天一,窦科峰.TCTP在肝癌细胞增殖过程中的作用及机制研究[J].,2020,(4):634-637 |
| TCTP在肝癌细胞增殖过程中的作用及机制研究 |
| Effects and Mechanisms of Translationally Controlled Tumor Protein on the Proliferation of Hepatoma Cells |
| 投稿时间：2019-09-23 修订日期：2019-10-18 |
| DOI：10.13241/j.cnki.pmb.2020.04.007 |
| 中文关键词: 翻译控制肿瘤蛋白 肝癌 增殖 AKT ERK |
| 英文关键词: Translationally controlled tumor protein Hepatocellular carcinoma Proliferation AKT ERK |
| 基金项目:国家重点研发计划项目（2016YFC0905902） |
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| 中文摘要: |
| 摘要 目的：研究翻译控制肿瘤蛋白(TCTP) 在肝癌细胞增殖过程中的作用及相关机制。方法：通过western blot技术检测14对肝癌与癌旁组织中TCTP的蛋白表达水平。通过siRNA（small interference RNA）技术在肝癌细胞系SMMC-7721和BEL-7404中下调TCTP的表达 ，然后通过CCK-8实验、克隆形成实验和EdU实验观察下调TCTP对肝癌细胞增殖的影响。通过western blot技术分析TCTP促进肝癌发生这一过程中可能涉及的分子通路。结果：相比于对应的癌旁组织，TCTP在肝癌组织中显著高表达。用siRNA技术下调TCTP水平后能够明显抑制肝癌细胞的增殖能力。下调TCTP的表达之后，AKT和ERK蛋白的磷酸化水平也随之降低。结论：TCTP在肝癌组织中显著高表达，并且在肝癌细胞的增殖过程中发挥着极其重要作用，其作用机制可能与AKT和ERK通路的磷酸化激活有关。 |
| 英文摘要: |
| ABSTRACT Objective: To study the effects and mechanisms of translation control protein (TCTP) on the proliferation of hepatocellular carcinoma (HCC) cells. Methods: Western blot was used to detect the protein levels of TCTP in 14 pairs of liver cancer and adjacent tissues. Small interference RNA was used to down-regulate TCTP in liver cancer cell lines SMMC-7721 and BEL-7404, and CCK-8 assays, colony formation assays and EdU assays were used to observe the effect of TCTP-knockdown on the proliferation of liver cancer cells. Western blot was used to analyze the possible molecular pathways involved in TCTP's role in promoting the proliferation of hepatoma carcinoma cells. Results: Compared with the corresponding para-cancer tissues, TCTP was significantly over-expressed in liver cancer tissues. TCTP-knockdown by siRNA can significantly inhibit the proliferation of liver cancer cells. Furthermore, phosphorylation of AKT and ERK were hampered by the down-regulation of TCTP. Conclusion: TCTP is highly expressed in HCC tissues and plays a crucial role in the proliferation of HCC cells, which may be related to the activation of AKT and ERK pathways. |
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