| 刘 玄,侯汶青,付 洪,谢博文,李古兵,郭泰林.纳米形貌诱导干细胞成骨分化的相关信号通路[J].,2020,(4):619-623 |
| 纳米形貌诱导干细胞成骨分化的相关信号通路 |
| Nano-topography Induce Signaling Pathways Involved in Stem Cell Osteoblastic Differentiation |
| 投稿时间：2019-05-23 修订日期：2019-06-18 |
| DOI：10.13241/j.cnki.pmb.2020.04.004 |
| 中文关键词: 骨髓间充质干细胞 纳米形貌 信号通路 |
| 英文关键词: BMSC Nano-topography Signaling Pathways |
| 基金项目:中国国家重点研究发展计划项目（2016YFB0700803）；学生研究培训项目（201810613078）；四川省科技厅基金项目（2018SZYZF0012） |
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| 中文摘要: |
| 摘要 目的：探究纳米形貌诱导间充质干细胞（MSC）分化中的作用以及相关分子机制。方法：利用阳极氧化法制备二氧化钛纳米管形貌，使用qRT-PCR技术，RNA-seq技术，分析接种在纳米形貌表面的间充质干细胞的基因表达情况。并筛选对成骨相关的信号通路中的成员，观察他们基因上调或下调情况。结果：在钛金属表面构建出了纳米形貌，利用实时定量PCR确定了成骨相关的基因：碱性磷酸酶（ALP），骨桥蛋白（OPN）和骨钙素（OCN）相比没有纳米形貌的钛片上培养的细胞均发生上调。通过对这些基因相关的成骨信号通路进行转录组数据分析（筛选基因P<0.05），发现在BMP2信号通路中的相关蛋白基因表达没有太大变化，同时Notch以及Wnt非经典信号通路中相关蛋白基因发生较为明显变化。结论：通过分析间充质干细胞成骨分化相关基因，以及转录组数据分析表明在纳米形貌诱导BMSC分化过程中，相对于平坦的表面，纳米形貌启动了Notch以及非经典的Wnt信号通路，因此表现出更加优良的促成骨分化的效果。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the role and molecular mechanism of nano-topography induced MSC osteoblastic differentiation. Methods: The nanotopography of the titanium surface was prepared by anodization. qRT-PCR been used analyzed osteoblastic related gene expression. RNA sequencing obtained transcriptome data and screened the results(P<0.05). Changes of gene expression in the osteoblastic -related signaling pathway were observed. Results: Nano-topography was constructed on the surface of titanium. Osteoblastic-related genes (alkaline phosphatase (ALP), osteopontin (OPN) and osteocalcin (OCN)) were up-regulated (vs. cells on flat Ti) by real-time quantitative PCR. By analyzing the RNA sequencing data of these gene-related osteoblastic signaling pathways, it was found that the related protein genes in Notch and Wnt non-canonical signaling pathways changed significantly. Conclusion: By analyzing the genes related to osteoblastic differentiation of BMSC and RNA sequencing data, it is shown that in the process of Nano-topography-induced BMSC differentiation, Nano-topography up-regulated Notch and non-canonical Wnt signaling pathways compare with the flat surface. Therefore, it exhibits a more excellent effect of promoting bone differentiation. |
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