| 赵 静,杨兴武,王 旗,王 亮,王国泰,嵇文波,陈国仓,王 鑫.虎杖提取物对人胰腺癌细胞系Panc-1增殖与凋亡的影响[J].,2020,(1):50-54 |
| 虎杖提取物对人胰腺癌细胞系Panc-1增殖与凋亡的影响 |
| Effects of Polygonum Cuspidate Extract on the Proliferation and Apoptosis of Human Pancreatic Cancer Cell Line Panc-1 |
| 投稿时间：2019-04-24 修订日期：2019-05-24 |
| DOI：10.13241/j.cnki.pmb.2020.01.010 |
| 中文关键词: 虎杖 胰腺癌 Panc-1 细胞增殖 细胞凋亡 |
| 英文关键词: Polygonum cuspidatum Pancreatic cancer Panc-1 Cell proliferation Apoptosis |
| 基金项目:咸阳市科技局科研项目（2018KT-23） |
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| 中文摘要: |
| 摘要 目的：通过虎杖提取物干预人胰腺癌细胞系Panc-1，探讨虎杖提取物对人胰腺癌细胞 增殖凋亡表型的影响。方法：制备不同浓度（0、10、50、100、150、200 μg/mL）的虎杖提取物，将各个浓度的虎杖提取物分别加入待处理的人胰腺癌Panc-1细胞系中持续培养24 h后，利用CCK-8（cell counting kit-8）法检测细胞株Panc-1的增殖活性；将100 μg/mL虎杖提取物处理人胰腺癌细胞系Panc-1 24 h后，利用流式细胞术（FCM）检测其细胞周期及凋亡分布；100 μg/mL虎杖提取物处理人胰腺癌细胞株Panc-1 24 h后，提取细胞总RNA及总蛋白，后续利用实时荧光定量PCR及Western blot分别检测人胰腺癌细胞株Panc-1增殖标志基因PCNA、CDK2及凋亡标志基因BAD、BAX的转录和翻译水平。结果：CCK-8结果表明虎杖提取物对人胰腺癌细胞系Panc-1细胞增殖的抑制率随浓度增加；流式细胞术结果显示虎杖提取物抑制人胰腺癌细胞增殖促进其凋亡；荧光定量PCR和Western blot结果显示虎杖提取物能使人胰腺癌细胞增殖标志基因PCNA，CDK2表达量下降，凋亡标志基因BAD，BAX表达量上升。结论：虎杖提取物能够抑制人胰腺癌细胞系Panc-1细胞增殖并促进其凋亡。 |
| 英文摘要: |
| ABSTRACT Objective: The effect of Polygonum cuspidatum extract on Pancreatic cancer cell line Panc-1 was investigated by extracting Polygonum cuspidatum extracts into human pancreatic cancer cell proliferation and apoptosis phenotype. Methods: Different concentrations (0, 10, 50, 100, 150, 200 μg/mL) of Polygonum cuspidatum extract were prepared, and each concentration of Polygonum cuspidatum extract was added to the human pancreatic cancer Panc-1 cell line to be treated for 24 h. The proliferation activity of the cell line Panc-1 was detected by CCK-8 (cell counting kit-8) method; the human pancreatic cancer cell line Panc-1 was treated with 100 μg/mL Polygonum cuspidatum extract for 24 h, and then flow cytometry was used ( FCM) was used to detect the cell cycle and apoptosis distribution; 100 μg/mL Polygonum cuspidatum extract was used to treat human pancreatic cancer cell line Panc-1 for 24 h, and the total RNA and total protein were extracted. Subsequent real-time PCR and Western blot were used to detect human pancreatic cancer. Transcription and translation levels of the cell line Panc-1 proliferation marker genes PCNA, CDK2 and apoptosis marker genes BAD, BAX. Results: The results of CCK-8 showed that the inhibition rate of Polygonum cuspidatum extract on the proliferation of human pancreatic cancer cell line Panc-1 increased with concentration. The results of flow cytometry showed that Polygonum cuspidatum extract inhibited the proliferation of human pancreatic cancer cells and promoted its apoptosis. The results of real-time PCR and Western blot showed The extract of Polygonum cuspidatum can decrease the expression of PCNA and CDK2 in human pancreatic cancer cell proliferation, and the expression of apoptosis marker gene BAD and BAX increased. Conclusion: Polygonum cuspidatum extract can inhibit the proliferation and promote apoptosis of human pancreatic cancer cell line Panc-1. |
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