| 郭云山,郝定均,王晓东,胡慧敏,黄研生,章雪芳,薛留洁,张新亮.STIM1调控细胞运动促进骨肉瘤转移的研究[J].,2019,19(24):4601-4606 |
| STIM1调控细胞运动促进骨肉瘤转移的研究 |
| STIM1 Promotes the Metastasis of Osteosarcoma via Regulating the Cell Movement |
| 投稿时间：2019-06-06 修订日期：2019-06-30 |
| DOI：10.13241/j.cnki.pmb.2019.24.001 |
| 中文关键词: STIM1 细胞运动 骨肉瘤 转移 |
| 英文关键词: STIM1 Movement Osteosarcoma Metastasis |
| 基金项目:国家自然科学基金青年科学基金项目(81502330)；中央高校基本科研业务费专项资金资助项目(xzy012019123)；陕西省博士后科研项目资助(2017BSHQYXMZZ12) |
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| 中文摘要: |
| 摘要 目的：明确STIM1是否参与调控细胞运动促进骨肉瘤的转移。方法：应用靶向STIM1的siRNA沉默MG-63骨肉瘤细胞中STIM1的表达，然后用侵袭实验、迁移实验以及黏附实验检测骨肉瘤细胞侵袭、迁移与黏附能力的变化，采用Western Blot检测细胞FAK和paxillin的表达及Rac1和RhoA信号通路的活性。结果：转染靶向STIM1的siRNA后，MG-63骨肉瘤细胞中STIM1的蛋白表达和mRNA表达均明显降低(P<0.05)，细胞的侵袭、迁移与黏附能力均显著下降(P<0.05)，细胞伪足与细胞骨架的重要组分FAK和paxillin的表达及调控细胞运动的Rac1和RhoA信号通路活性均显著降低(P<0.05)。结论：STIM1可能通过激活RhoA和Rac1的信号通路，增加FAK和paxillin的表达，从而调控骨肉瘤细胞运动，促进骨肉瘤转移。 |
| 英文摘要: |
| ABSTRACT Objective: The aim of this study was to determine whether STIM1 is involved in the regulation of cellular movement in the promotion of osteosarcoma metastasis. Methods: The expression of STIM1 was silenced by siRNA targeting STIM1 in MG-63 osteosarcoma cells. Then the invasion experiment, migration experiment and adhesion experiment were used to detect the changes in the ability of invasion, migration and adhesion of osteosarcoma cells. The expression of FAK and paxillin, as well as the activity of RhoA and Rac1signaling pathway were detected by Western Blot. Results: The expression and transcription of STIM1 in MG-63 osteosarcoma cells were significantly reduced after we transfected siRNA targeting STIM1(P<0.05). Then, we found that the invasion, migration and adhesion abilities of MG-63 osteosarcoma cells were significantly decreased after silencing STIM1 expression in MG-63 osteosarcoma cells(P<0.05). In addition, the levels of FAK and paxillin, important components of cellular pseudopod and cytoskeleton, were also significantly lowered(P<0.05). Further studies showed that silencing STIM1 expression in MG-63 osteosarcoma cells significantly inhibited the activity of RhoA and Rac1signaling pathways(P<0.05). Conclusion: STIM1 activates RhoA and Rac1signaling pathways to increase expression of FAK and paxillin, thus regulate osteosarcoma cell movement and promote osteosarcoma metastasis. |
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