| 殷艳蓉,朱萧玲,常 盼,邢书娟,董明清,吕 颖.外周血miR-223和血清BNP水平在慢性心力衰竭患者中的诊断价值[J].,2019,19(22):4344-4347 |
| 外周血miR-223和血清BNP水平在慢性心力衰竭患者中的诊断价值 |
| Diagnostic Value of Peripheral Blood miR-223 and Serum BNP Levels for the Patients with Chronic Heart Failure |
| 投稿时间：2019-08-08 修订日期：2019-08-31 |
| DOI：10.13241/j.cnki.pmb.2019.22.031 |
| 中文关键词: 心力衰竭 miR-223 B型利钠肽 诊断 |
| 英文关键词: Heart failure miR-223 B-type natriuretic peptide Diagnosis |
| 基金项目:国家自然科学基金面上项目（81670365） |
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| 中文摘要: |
| 摘要 目的：探究慢性心力衰竭（chronic heart failure，CHF）患者外周血microRNA-223（miR-223）和血清B型利钠肽（B-type natriuretic peptide，BNP）水平及其诊断价值。方法：选取CHF患者65例（CHF组），其中美国纽约心脏病协会（New York Heart Association，NYHA）心功能分级Ⅱ级24例，Ⅲ级22例，Ⅳ级19例。另取40例同期在体检中心进行健康体检者（Control组），采用实时荧光定量PCR检测外周血中miR-223水平，ELISA检测血清BNP含量，ROC曲线评价miR-223及BNP对CHF的诊断价值。结果：CHF组左心室短轴缩短率及左心室射血分数显著低于Control组（P＜0.01）；左心室舒张末期内径显著高于Control组（P＜0.01）。CHF组不同NYHA心功能分级患者miR-223和BNP水平均高于Control组，且miR-223和BNP水平随NYHA心功能分级逐渐递增，组间两两比较均显示统计学差异（P＜0.05）。miR-223诊断CHF的曲线下面积（area under the curve，AUC）为0.7375，临界值为43.51时灵敏度为92.82%，特异度为89.44%；BNP诊断CHF的AUC为0.7925，临界值为128 ng/L时灵敏度为92.93%，特异度为92.58%。结论：CHF患者外周血miR-223和血清BNP水平高于健康对照人群，其对CHF的诊断具有一定的临床参考价值。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the level of microRNA-223 (miR-223) in peripheral blood and serum B-type natriuretic peptide (BNP) level of patients with chronic heart failure (CHF), and to analyze their diagnostic value. Methods: Sixty-five patients with CHF were enrolled in the CHF group: 24 patients with New York Heart Association (NYHA) functional class II, 22 with class III and 19 with class IV. Another 40 healthy subjects who underwent a physical examination were included in the control group. The level of miR-223 in peripheral blood was measured by real-time quantitative PCR. The serum BNP was determined by ELISA. The ROC was used to evaluate the diagnostic value of miR-223 and BNP for CHF. Results: Compared to the control group, the left ventricular ejection fraction and fractional shortening were significantly lower, left ventricular end-diastolic diameter was significantly wider in the CHF group (all P<0.01). The level of miR-223 or BNP in the patients with CHF was higher than that in the control group (P<0.05), and the level of miR-223 or BNP gradually increased with NYHA functional class. The area under the curve (AUC) of miR-223 to diagnose CHF was 0.7375; while the cutoff of miR-223 was 43.51, sensitivity and specificity were 92.82% and 89.44%. The AUC of BNP was 0.7925; while the cutoff of BNP was 128 ng/L sensitivity and specificity were 92.93% and 92.58%. Conclusion: The level of miR-223 in peripheral blood and serum BNP level of patients with CHF are up-regulated, which had a good diagnostic value for CHF. |
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