| 梁婉琪,赵宗霞,李芬霞,常丽花,杨淑梅.MicroRNA-21在卵巢癌病灶转移过程中的作用及机制研究[J].,2019,19(21):4137-4142 |
| MicroRNA-21在卵巢癌病灶转移过程中的作用及机制研究 |
| Role and Mechanism of MicroRNA-21 in the Process of Ovarian Cancer Lesion Metastasis |
| 投稿时间：2019-02-27 修订日期：2019-03-23 |
| DOI：10.13241/j.cnki.pmb.2019.21.031 |
| 中文关键词: microRNA-21 卵巢癌 转移 侵袭 上皮间质转化 |
| 英文关键词: microRNA-21 Ovarian cancer Metastasis Invasion EMT |
| 基金项目:陕西省科技统筹基金项目(2016KTZDSF01-013-04) |
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| 中文摘要: |
| 摘要 目的：探讨microRNA-21在卵巢癌病灶转移过程中的作用及其机制。方法：选取本院2016年6月-2018年5月收治的138例卵巢癌患者，其中63例出现结直肠转移，75例未发现有转移。qRT-PCR分别检测两组患者肿瘤组织、癌旁组织和正常组织中microRNA-21的表达；Western blot检测两组患者肿瘤组织中PGDH、PGE2、Twist表达。通过转染过表达载has-microRNA-21上调A2780细胞中microRNA-21的表达，采用平板克隆实验检测细胞克隆形成能力，Trans-well细胞迁移实验和侵袭实验分别检测细胞迁移和侵袭能力。Western blot检测PGDH、PGE2、Twist蛋白表达。结果：卵巢癌转移组肿瘤组织中 microRNA-21表达高于未转移组、癌旁组织和正常卵巢组织(P<0.05)，卵巢癌转移组肿瘤组织中PGDH表达低于未转移组，而PGE2、Twist表达高于未转移组(P<0.05)。microRNA-21过表达的A2780细胞平板克隆形成能力、迁移和侵袭能力及上皮间质转化相关蛋白PGE2和Twist表达均明显高于阴性对照组(P<0.05)，而PGDH表达的表达明显降低(P<0.05)。结论：microRNA-21可能通过抑制PGDH的表达增加PGE2的表达，进而激活上皮间质转化，促进卵巢癌转移。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the role and mechanism of microRNA-21 in the metastasis of ovarian cancer. Methods: A total of 138 patients with ovarian cancer admitted to our hospital from June 2016 to May 1818 were enrolled. Among them, 63 had colorectal metastasis and 75 had no metastasis. The expression of microRNA-21 in tumor tissues, paracancerous tissues and normal tissues of the two groups was detected by qRT-PCR. The expressions of PGDH, PGE2 and Twist in the tumor tissues of the two groups were detected by Western blot. The expression of microRNA-21 in A2780 cells was up-regulated by transfection over-expression of has-microRNA-21. The ability of cell clone formation was detected by plate cloning assay. Trans-well cell migration assay and invasion assay were used to detect cell migration and invasion. Western blot was used to detect the expression of PGDH, PGE2 and Twist proteins. Results: The expression of microRNA-21 in the ovarian cancer metastasis group was higher than that in the non-metastasis group, paracancerous tissues and normal ovarian tissues (P<0.05). The expression of PGDH in the ovarian cancer metastasis group was lower than that in the non-metastasis group, but PGE2. Twist expression was higher than that of the non-metastatic group (P<0.05). The expression, migration and invasion ability of the microRNA-21 overexpressing A2780 cells were significantly higher than those of the negative control group (P<0.05), while the expression of PGDH was significantly decreased (P<0.05). Conclusion: microRNA-21 may increase the expression of PGE2 by inhibiting the expression of PGDH, thereby activates the epithelial mesenchymal transition and promotes the ovarian cancer metastasis. |
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