| 牛 舜,龙 华,李永伟,赵 振,马保安.人工关节假体无菌性松动动物模型的快速建立[J].,2019,19(20):3818-3822 |
| 人工关节假体无菌性松动动物模型的快速建立 |
| Rapid Establishment of Artificial Joint Aseptic Loosening Animal Model |
| 投稿时间：2019-03-23 修订日期：2019-04-18 |
| DOI：10.13241/j.cnki.pmb.2019.20.004 |
| 中文关键词: 超高分子量聚乙烯 动物模型 人工关节置换 无菌性松动 |
| 英文关键词: UHMWPE Animal model Arthroplasty Aseptic loosening |
| 基金项目:国家自然科学基金项目（81401825）；空军军医大学科技发展基金资助项目（2019XC063）；唐都医院科技创新发展基金项目（2018LCYJ013） |
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| 中文摘要: |
| 摘要 目的：人工关节置换手术发展至今，取得了非常大的成功，已在临床解决了许多终末期的关节疾患。研究表明，假体无菌性松动的患者，其假体周围处于高骨更新状态。因此骨-假体界面的早期整合对于阻止磨屑颗粒和细胞因子的迁移至关重要，而限制人工关节假体使用寿命的一个主要原因是磨屑颗粒诱导的假体无菌性松动。在本研究中，我们旨在尝试使用107 个UHMWPE颗粒快速建立人工关节假体无菌性松动的兔动物模型，以便为进一步探讨假体无菌性松动的机制及防治奠定实验基础。方法：我们用雌性新西兰大白兔建立动物模型，随机均分为实验组和对照组，在两组动物的左侧胫骨髓腔内植入羟基磷灰石（hydroxyapatite，HA）涂层假体。在实验组中，分别于假体表面和膝关节腔内植入0.5×107超高分子量聚乙烯（Ultra-high molecular weight polyethylene，UHMWPE）颗粒。结果：同对照组相比，实验组动物的关节内压力更高、骨组织形态学参数更差、假体生物力学固定强度更低，同时，促炎细胞因子和骨更新标志均显著高于对照组。结论：我们使用很少的UHMWPE颗粒成功快速地建立了人工关节假体无菌性松动的兔动物模型，从而为今后人工关节假体无菌性松动的进一步研究奠定了实验基础。 |
| 英文摘要: |
| ABSTRACT Objective: Artificial joint arthroplasty has achieved great success till now, which has treated many late stage joint-related diseases. Studies have showed that the peri-implant environment was in the status of high bone turnover rate in the patients suffering from aseptic loosening. Therefore, early bone-implant integration is important for inhibiting the migration of particles and cytokines. Wear debris-induced aseptic loosening is a major cause of artificial prostheses failure. In this study, we tried to use only 107 particles to establish artificial joint aseptic loosening rabbit model, which may lay the experimental foundation for further studying the mechanism and the prevention of aseptic loosening. Methods: We used female New Zealand white rabbits to establish animal model. Animals were randomly divided into an experimental group and a control group, and HA-coated implants were inserted into the left tibias of both groups. In the experimental group, 0.5×107 UHMWPE wear particles were injected both around implants and into knee joints. Results: Compared to the control group, animals in the experiment group presented higher intra-articular pressure, poorer bone architecture, lower biomechanical properties, and higher levels of pro-inflammatory cytokines and bone turnover markers. Conclusion: We used fewer UHMWPE particles to establish artificial joint aseptic loosening rabbit model successfully and quickly, which laid the experimental foundation for further aseptic loosening related studies. |
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