| 吴小秦,谭业农,唐旭霞,王 翔,符国庆,李淑芳.BRD4在喉鳞状细胞癌病灶转移过程中的作用及机制研究[J].,2019,19(19):3738-3743 |
| BRD4在喉鳞状细胞癌病灶转移过程中的作用及机制研究 |
| Role and Mechanism of BRD4 in the Process of Focal Squamous Cell Carcinoma Metastasis |
| 投稿时间：2019-01-28 修订日期：2019-02-23 |
| DOI：10.13241/j.cnki.pmb.2019.19.032 |
| 中文关键词: BRD4 喉鳞状细胞癌 转移 侵袭 上皮间质转化 |
| 英文关键词: BRD4 Laryngeal Squamous Cell Carcinoma Metastasis Invasion EMT |
| 基金项目:海南省自然科学基金面上项目(SQ2017SHFZ0093) |
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| 中文摘要: |
| 摘要 目的：探讨溴样结构域蛋白4 (Bromoid Domain Protein 4，BRD4)在喉鳞状细胞癌病灶转移过程中的作用及其机制。方法：收集本院2016年4月-2018年4月收治的87例喉鳞状细胞癌患者手术标本。qRT-PCR、Western blot和免疫组化染色检测患者肿瘤组织（Tumor Tissue）、癌旁组织(Adjacent Tissue)和正常组织(Normal Tissue)中BRD4表达；BRD4 拮抗剂 GSK525762A（500 nmoL/L）处理喉鳞状细胞癌Hep2细胞，24、48和72 h后CCK-8检测细胞增殖；Trans-well细胞迁移实验和侵袭实验分别检测GSK525762A给药前后Hep2细胞迁移和侵袭能力；qRT-PCR 和Western blot检测GSK525762A给药前后Hep2细胞BRD4、E-Cadherin、N-Cadherin 和Twist蛋白表达。结果：喉鳞状细胞癌肿瘤组织中 BRD4表达高于癌旁组织和正常卵巢组织(P<0.05)；GSK525762A给药处理后Hep2细胞增殖能力、迁移和侵袭能力及BRD4和上皮间质转化相关蛋白N-Cadherin 和Twist表达均明显低于阴性对照组(P<0.05)，而E-Cadherin表达的表达明显升高(P<0.05)。结论：BRD4可能通过抑制E-Cadherin的表达，增加N-Cadherin 和Twist的表达，进而激活上皮间质转化，促进喉鳞状细胞癌转移。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the role and mechanism of BRD4 in the process of focal squamous cell carcinoma metastasis. Methods: Eighty-six surgical specimens of patients with laryngeal squamous cell carcinoma admitted from April 2016 to April 2018 were enrolled. qRT-PCR, Western blot and immunohistochemical staining were used to detect BRD4 expression in tumor tissues, paracancerous tissues and normal tissues; BRD4 antagonist GSK525762A (500 nmoL/L) was used to treat laryngeal squamous cell carcinoma Hep2 cells, 24, 48 and 72 After C, CCK-8 was used to detect cell proliferation; Trans-well cell migration assay and invasion assay were used to detect the migration and invasion of Hep2 cells before and after GSK525762A administration; qRT-PCR and Western blot were used to detect E-Cadherin, N-Cadherin and Twist in Hep2 cells before and after GSK525762A administration. Results: The expression of BRD4 in laryngeal squamous cell carcinoma was higher than that in adjacent normal tissues and normal ovarian tissues (P<0.05). The proliferation, migration and invasion of Hep2 cells and the protein associated with epithelial-mesenchymal transition were regulated by GSK525762A. The expression of Cadherin and Twist was significantly lower than that of the negative control group (P<0.05), while the expression of E-Cadherin was significantly increased (P<0.05). Conclusion: BRD4 may increase the expression of N-Cadherin and Twist by inhibiting the expression of E-Cadherin, thereby activating epithelial-mesenchymal transition and promoting the metastasis of laryngeal squamous cell carcinoma. |
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