| 韩 鹏,王延辉,王 萱,张 婷,牛晓蓉,陈阳静,王林古,张少强.西北地区汉族人核苷酸剪切修复蛋白的表达与头颈鳞癌发病风险的相关性研究[J].,2019,19(18):3494-3498 |
| 西北地区汉族人核苷酸剪切修复蛋白的表达与头颈鳞癌发病风险的相关性研究 |
| A Study on the Associations between the Expression Levels of Core Nucleotide Excision Repair Proteins in Lymphocytes and Risk of Squamous Cell Carcinoma of the Head and Neck in Northwest Chinese Population |
| 投稿时间：2019-04-28 修订日期：2019-05-23 |
| DOI：10.13241/j.cnki.pmb.2019.18.020 |
| 中文关键词: 头颈部鳞状细胞癌 核苷酸剪切修复蛋白 DNA 修复 |
| 英文关键词: Squamous cell carcinoma of head and neck Nucleotide excision repair proteins DNA repair |
| 基金项目:西安交通大学第一附属医院青年创新基金(2017QN-11)；中央高校基本科研业务费资助(xjj2018094)；国家自然科学基金青年科学基金项目(81833770) |
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| 中文摘要: |
| 摘要 目的：初步探讨西北地区汉族人核苷酸剪切修复蛋白表达水平与头颈鳞癌发病风险的相关性，从翻译水平为头颈鳞癌提供新的筛检标志物。方法：收集118例头颈鳞癌患者和88例健康对照，均为西北地区汉族人。通过反向蛋白芯片实验检测研究对象外周血淋巴细胞中的5个核心核苷酸剪切修复蛋白的相对表达水平，采用卡方检验分析两组间一般特征的差异，并计算蛋白相对表达水平间的差异，logistic回归计算OR值及95% CI，最后通过绘制接受者操作特性曲线评价模型的诊断价值。结果：病例组XPB (Xeroderma pigmentosum, complementation group B)的表达水平显著低于对照组(P = 0.013)。Logistic回归分析结果显示XPB高表达者相比，其低表达者头颈鳞癌患病风险的OR为1.74(95％CI，0.99-3.06)。此外，XPB的蛋白表达水平降低与SCCHN风险增加之间存在剂量反应关系(P trend = 0.042)。最后，我们通过接受者操作特性曲线计算曲线下面积，评估XPB表达水平的效应对于头颈鳞癌易感性筛检能力。包含XPB表达水平的效应模型中曲线下面积显著改善(P = 0. 048)。结论：在西北地区汉族人中XPB的相对表达水平的降低与头颈鳞癌患病风险的增加相关。XPB表达水平的降低可能在既往吸烟者的头颈鳞癌患病风险中发挥更重要的作用。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the association between expression levels of Nucleotide excision repair (NER) proteins and risk of the squamous cell carcinoma of head and neck (SCCHN) in northwest Chinese population. Methods: The 118 SCCHN patients and 88 cancer-free controls were recruited from northwest part of China. The expression levels of five core NER proteins were tested by reverse-phase protein lysate microarrays assay. The Chi-square test was used to evaluate differences in demographic variables between cases and controls. Then, we compared differences in the protein expression levels. The associations of NER proteins expression levels with SCCHN risk were analyzed by computing ORs and CIs from logistic regression model. To assess the improvement of SCCHN risk models, we compared the ROC curve among two risk models. Results: Compared with the controls, patients had lower expression levels of Xeroderma pigmentosum complementation group B (XPB) (P = 0.013). After dividing the subjects by controls' median expression levels, we found an association between an increased risk of SCCHN and low XPB expression levels [OR and 95% CI: 1.74 and 0.99-3.06, compared with the lower expression level; P trend = 0.042]. When we assessed prediction models integrating demographic variables and protein expression levels on SCCHN risk, the sensitivity of the expanded model was significantly improved with the model including XPB expression level(P = 0. 048). Conclusion: Reduced XPB expression levels were associated with an increased risk of SCCHN in northwest Chinese population. |
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