| 付 凯,王 巨,王雪峰,张 健,姜久仰,刘宏宇.食管鳞癌血管生成拟态的组织学和细胞学研究[J].,2019,19(17):3234-3238 |
| 食管鳞癌血管生成拟态的组织学和细胞学研究 |
| Histology and Cytology Research on the Vasculogenic Mimicry in the Esophageal Squamous Cell Carcinomas |
| 投稿时间：2019-04-30 修订日期：2019-05-23 |
| DOI：10.13241/j.cnki.pmb.2019.17.007 |
| 中文关键词: 食管鳞癌 血管生成拟态 预后 |
| 英文关键词: Esophageal squamous cell carcinoma Vasculogenic mimicry Prognosis |
| 基金项目:黑龙江省卫生计生委科研项目(2014-294) |
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| 中文摘要: |
| 摘要 目的：探讨血管生成拟态(vasculogenic mimicry，VM)与食管鳞癌临床病理特征的关系及其对患者预后的影响，并分析食管癌血管生成拟态的形成机制。方法：收集57例食管鳞癌石蜡包埋样本，进行过碘酸雪夫氏(PAS)及CD34免疫组织化学双重染色，结合HE染色，观察食管鳞癌血管生成拟态的发生情况。对患者临床病理和预后信息进行单因素分析，Kaplan-Meier生存比较和Cox风险模型分析。通过食管鳞癌细胞株Eca-109三维培养建立，观察RNAi沉默VE-cadherin对食管鳞癌Eca109血管生成拟态形成的影响。结果：食管鳞癌中VM表达的阳性率为54.3%，显著高于正常食管黏膜组织；VM在病理分型为低分化食管鳞癌的阳性表达率为78.9%，显著高于中高分化组(P<0.05)；III-Ⅳ期食管鳞癌患者VM阳性率显著高于Ⅰ-Ⅱ期食管鳞癌患者(P<0.05)；有淋巴结转移的食管鳞癌者VM阳性率明显高于无淋巴结转移者(P<0.05)。单因素分析结果显示食管鳞癌VM的发生率与肿瘤的分化程度、TNM分期和淋巴转移显著相关。Kaplan-Meier生存分析显示有VM组食管鳞癌患者的生存期明显短于无VM组(P<0.05)；Cox分析显示VM是影响食管鳞癌患者预后的独立危险因素(RF=0.67)。三维培养结果显示Eca-109细胞在基质胶上形成典型的血管网状样结构，VE-cadherin-siRNA可有效抑制VE-cadherin在Eca109的表达，抑制体外培养的Eca109细胞VM的形成。结论：血管生成拟态是食管鳞癌一种独特的血液供应模式，与食管鳞癌的分化程度、TNM分期、淋巴转移密切相关，是食管鳞癌患者术后生存期的独立危险因素。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the relationship between vasculogenic mimicry(Vasculogenic mimicry, VM) and clinicopathological features of esophageal squamous cell carcinoma and its influence on the prognosis of the patients, and analyze the mechanism of VM formation in the esophageal cancer. Methods: A total of 57 cases of ESCC paraffin bag buried samples and periodic acid Schiff (PAS) and CD34 immunohistochemical double staining, combined with HE staining were selected, the occurrence of vasculogenic mimicry in esophageal squamous cell carcinoma was observed. Combined with the clinical pathology and prognosis information, the correlation parameters were analyzed by univariate analysis, Kaplan-Meier survival comparison and Cox risk model analysis. Through the three-dimensional culture of esophageal squamous cell carcinoma cell line Eca-109 were established in the effect of RNAi silencing of VE cadherin in esophageal squamous cell carcinoma Eca109 vasculogenic mimicry formation. Results: The positive rate of VM expression in the esophageal squamous cell carcinoma was 54.3%, which was significantly higher than that in the normal esophageal mucosa; The positive expression rate of VM expression in the poorly differentiated squamous cell carcinoma was 78.9%, which was significantly higher than that in highly differentiated group (P<0.05). The positive expression rate of VM expression in the Stage III-IV esophageal squamous cell carcinoma was significantly higher than that of esophageal squamous cell carcinoma patients with stage I and II (P<0.05). The positive expression rate of VM expression in the esophageal squamous cell carcinoma with lymph node metastasis was significantly higher than those without lymph node metastasis (P<0.05). Univariate analysis showed that the incidence of VM in esophageal squamous cell carcinoma was closely related to the degree of tumor differentiation, TNM stage and lymph node metastasis. Kaplan-Meier survival analysis showed that there was significantly shorter survival than no VM group, Cox analysis showed that VM was an independent risk factor for the prognosis of patients with esophageal squamous cell carcinoma (RF=0.67, VM). Three dimensional culture results showed that Eca-109 cells in Matrigel formed typical vascular network like structure. VE-cadherin-siRNA can effectively inhibit VE-cadherin expression in Eca109, inhibit the formation of in vitro cultured Eca109 cell VM. Conclusion: Vasculogenic mimicry isunique pattern of blood supply that is closely related to the differentiation, TNM stage, lymph node metastasis of esophageal squamous cell carcinoma, and it is an independent risk factor for survival in the postoperative patients with esophageal squamous cell carcinoma. |
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