| 陈 婷,侯 晋,李心竹,宋 词,张雪洋,吴补领.三维联合培养牙髓细胞和血管内皮祖细胞促进成牙本质向/成骨向分化[J].,2019,19(16):3028-3032 |
| 三维联合培养牙髓细胞和血管内皮祖细胞促进成牙本质向/成骨向分化 |
| Three-dimensional Coculture of Dental Pulp Cells and Endothelial Progenitor Cells Enhances Osteo/odontogenic Differentiation |
| 投稿时间：2019-05-06 修订日期：2019-05-30 |
| DOI：10.13241/j.cnki.pmb.2019.16.005 |
| 中文关键词: 牙髓细胞 血管内皮祖细胞 三维联合培养 成牙本质向/成骨向分化 |
| 英文关键词: Dental pulp cells Endothelial progenitor cells Three-dimensional coculture Osteo/odontogenic differentiation |
| 基金项目:国家自然科学基金项目（81800950；81870755；81670986） |
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| 中文摘要: |
| 摘要 目的：探讨三维联合培养牙髓细胞（dental pulp cells, DPCs）和血管内皮祖细胞(endothelial progenitor cells, EPCs)对成牙本质向/成骨向分化的影响。方法：取单独培养DPCs及联合培养的DPCs和EPCs进行三维培养后成牙本质向/成骨向诱导，使用茜素红染色及半定量分析、RT-PCR和细胞免疫荧光检测成牙本质向/成骨向分化能力。采用SPSS 23.0统计软件对数据进行统计学分析。结果：茜素红染色显示联合培养组和单独培养组之间未见显著差异。RT-PCR和细胞免疫荧光显示成牙本质向/成骨向相关基因mRNAs和蛋白表达水平联合培养组显著高于单独培养组。结论：三维联合培养的DPCs和EPCs促进成牙本质向/成骨向分化，为牙髓再生提供可能实验依据。 |
| 英文摘要: |
| ABSTRACT Objective: To observe the effect of three-dimensional coculture of dental pulp cells (DPCs) and endothelial progenitor cells (EPCs) on the osteo/odontogenic differentiation. Methods: After osteo/odontogenic induced of three-dimensional culture of the coculture of DPCs and EPCs and the culture of DPCs, Alizarin red stain, RT-PCR and immunofluorescence were used to detect the osteo/odontogenic differentiation. The data was analyzed using SPSS 23.0 software package. Results: Alizarin red stain showed that it is no significant differences between the coculture group and the culture group. RT-PCR and immunofluorescence showed that the coculture group is significant higher than the culture group on the mRNA and protein level of osteo/odontogenic differentiation. Conclusion: Three-dimensional coculture of dental pulp cells and endothelial progenitor cells enhances osteo/odontogenic differentiation. |
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