| 强子耘,陈凤收,贾 慧,李 哲,马 虹.舒芬太尼预处理对大鼠脊髓缺血再灌注损伤中炎性因子MPO，IL-6，IL-15的影响[J].,2019,19(16):3013-3017 |
| 舒芬太尼预处理对大鼠脊髓缺血再灌注损伤中炎性因子MPO，IL-6，IL-15的影响 |
| Effects of Sufentanil Pretreatment on MPO, IL-6 and IL-15 in Rats with Spinal Cord Ischemia-reperfusion Injury |
| 投稿时间：2019-01-08 修订日期：2019-01-31 |
| DOI：10.13241/j.cnki.pmb.2019.16.003 |
| 中文关键词: 舒芬太尼 脊髓 缺血再灌注损伤 IL-15 IL-6 |
| 英文关键词: Sufentanil Spinal Ischemia reperfusion injury IL-15 IL-6 |
| 基金项目:国家自然科学基金项目（81771342） |
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| 中文摘要: |
| 摘要 目的：探讨舒芬太尼预处理对大鼠脊髓缺血再灌注损伤模型中炎性因子MPO，IL-6，IL-15的影响。方法：健康SD雄性大鼠30只，随机分为假手术组（Sham组，n=10）；缺血再灌注组（IR组n=10）；舒芬太尼预处理5 μg/kg组（Suf5组，n=10）。采用动脉夹夹闭胸主动脉方法制备脊髓缺血再灌注模型。Tarlov法测大鼠运动评分，HE染色观察大鼠脊髓组织细胞形态，Western Blot法测脊髓组织中MPO的表达，ELISA法检测脊髓组织中IL-6，IL-15含量。结果：IR组Tarlov评分高于sham组，Suf5组Tarlov评分低于IR组。HE染色显微镜下见IR组脊髓组织内出现广泛的变性神经元，胞核固缩偏位碎裂，并有有空泡形成；Suf5组脊髓组织神经元损伤坏死数量减少，细胞核形态基本正常。Suf5组中MPO，IL-6，IL-15，含量均低于IR组，IR组中MPO，IL-6，IL-15含量均高于sham组，差异均有统计学意义（P<0.05）。结论：舒芬太尼能降低大鼠脊髓缺血再灌注损伤组织中MPO，IL-15，1L-6表达，减轻炎症损害，进而减轻脊髓缺血再灌注损伤。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the effects of sufentanil pretreatment on inflammatory cytokines like MPO, IL-6 and IL-15 in rats with spinal cord ischemia-reperfusion injury. Methods: Thirty healthy SD male rats were randomly divided into sham operation group (Sham group, n=10), ischemia-reperfusion group (IR group n=10), and sufentanil pretreatment 5 μg/kg group (Suf5 group, n=10). Spinal cord ischemia-reperfusion model was prepared by occlusion of thoracic aorta with arterial clamp. The postoperative motor scores of rats were measured by Tarlov method, and the morphology of spinal cord histocyte was observed by HE staining. The expression of MPO in spinal cord tissue was detected by Western Blot, and the contents of IL-6 and IL-15 in spinal cord histocyte of rats in each group were detected by ELISA. Results: Tarlov score in the IR group was higher than that of the sham group, and Tarlov score of the Suf5 group was lower than that of the IR group but higher than that of the sham group. Histological observation of the spinal cord: Under HE staining microscope, the spinal cord tissue of the extensive denatured neurons, inflammatory cell infiltration, karyopyknosis deviation, fragmentation, and dissolution as well as vacuolization were spotted in spinal cord tissue of the ischemia-reperfusion group; The number of spinal cord tissue neuron damage necrosis in the Suf5 group decreased, with nuclear morphology being basically normal. Determination of MPO activity as well as content of IL-6 and IL-15 in spinal cord tissue: The contents of MPO, IL-6 and IL-15 in Suf 5 group were all lower than those in the IR group, the difference of which was statistically significant (P<0.05); the contents of MPO, IL-6 and IL-15 in the IR group were all higher than those in the sham group, the difference of which was statistically significant (P<0.05). Conclusion: Sufentanil can reduce the expression of MPO, IL-15, and 1L-6 in rats' tissues injured by spinal cord ischemia-reperfusion, reduce inflammation damage, and alleviate spinal cord ischemia-reperfusion injury. |
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