| 李 鹏,胡 强,张 颖,王 娟,孙理华.MitoQ对高糖诱导的心肌细胞线粒体功能影响[J].,2019,19(14):2652-2656 |
| MitoQ对高糖诱导的心肌细胞线粒体功能影响 |
| Effect of MitoQ on High Glucose-induced Mitochondrial Function in Myocardial Cells |
| 投稿时间：2018-11-08 修订日期：2018-11-30 |
| DOI：10.13241/j.cnki.pmb.2019.14.010 |
| 中文关键词: 线粒体辅酶Q 心肌细胞 线粒体功能障碍 |
| 英文关键词: Mitochondrial coenzyme Q Cardiomyocytes Pressure overload Mitochondrial dysfunction |
| 基金项目:新疆维吾尔自治区自然科学基金项目(2016D01C244) |
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| 中文摘要: |
| 摘要 目的：探讨MitoQ对高糖诱导的心肌细胞线粒体功能影响。方法：常规获取与纯化SD大鼠新生仔鼠心肌细胞，分为对照组、高糖组、实验组。对照组用含10%血清的DMEM培养基(5.5 mmol/L葡萄糖)培养；高糖组用含血清的高糖DMEM培养基(33 mmol/L葡萄糖)培养；实验组用含血清的高糖DMEM培养基(33 mmol/L葡萄糖)和MitoQ。MTT法检测心肌细胞存活率，氯离子荧光探针检测细胞内氯离子浓度，流式细胞术检测各组心肌细胞凋亡率，超氧化物阴离子荧光染色检测心肌细胞活性氧(reactive oxygen，ROS)含量，利用ATP检测试剂盒检测心肌细胞中的ATP水平，Western blot法检测心肌细胞胱天蛋白酶3(caspase-3)蛋白水平。结果：与对照组相比，高糖组的心肌细胞增凋亡率、ROS产生、氯离子相对浓度均明显增加，ATP显著降低（P<0.05），细胞内caspase-3蛋白表达显著上升(P<0.05)；与高糖组相比，实验组凋亡率降低，ROS产生、细胞内caspase-3蛋白表达均显著降低(P<0.05)。结论：高糖会引起心肌细胞线粒体障碍，造成心肌细胞凋亡，MitoQ可降低细胞内ROS和caspase-3水平，抑制心肌细胞凋亡，改善心肌细胞线粒体功能。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of MitoQ on high glucose-induced mitochondrial function in cardiomyocytes. Methods: The neonatal rat cardiac myocytes of SD rats were routinely obtained and purified and were divided into control group, high sugar group and experimental group. The control group were cultured with 10% serum DMEM medium (5.5 mmol/L glucose); the high sugar group were cultured with high glucose DMEM medium containing serum (33 mmol/L glucose); the experimental group were treated with high glucose DMEM medium containing serum (33 mmol/L glucose) and MitoQ. The chlorine ion fluorescence probe were used to detect the concentration of intracellular chlorine ion, the rate of apoptosis were detected by flow cytometry, the content of active oxygen (ROS) was detected by superoxide anion fluorescence staining, detection of ATP levels in cardiomyocytes using the ATP detection kit, and caspase-3 protein levels were detected by Western blot. Level. Results: The relative concentration of chloride in the control group, the high sugar group and the experimental group were(2.14±0.44)%, (23.11±4.39)% and (5.20±0.92)% respectively. Compared with the control group, the cardiomyocytes in the high glucose group increased the apoptosis and increased the ROS production, the ATP levels of cardiomyocytes in the control group and the experimental group were significantly higher than those in the high glucose group(P<0.05), and the caspase-3 protein level in the cardiomyocytes increased significantly(P<0.05). Compared with the high glucose group, the experimental group was apoptotic. Decreased, ROS production decreased, and caspase-3 protein levels in cardiomyocytes were significantly decreased(P<0.05). Conclusion: High glucose can cause the mitochondrial disorder and apoptosis of myocardial cells. MitoQ can improve the mitochondrial function of myocardial cell via reducing the intracellular ROS and caspase-3 levels, inhibit the apoptosis of cardiomyocytes. |
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