| 韩俊柱,朱勋兵,武世伍,李徽徽,王 胜.3种间充质干细胞成软骨分化潜能比较[J].,2019,19(12):2252-2255 |
| 3种间充质干细胞成软骨分化潜能比较 |
| Comparison of Chondrogenic Differentiation Potential of Three Mesenchymal Stem Cells |
| 投稿时间：2019-01-29 修订日期：2019-02-22 |
| DOI：10.13241/j.cnki.pmb.2019.12.011 |
| 中文关键词: 干细胞 骨髓间充质干细胞 脂肪间充质干细胞 滑膜间充质干细胞 成软骨分化 |
| 英文关键词: Stem cells Bone marrow mesenchymal stem cells Adipose-derived mesenchymal stem cells Synovial mesenchymal stem cells Chondrogenic differentiation |
| 基金项目:安徽省教育厅自然科学基金项目（KJ2018A0243）；蚌埠医学院科技发展基金项目（BYKF17112） |
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| 中文摘要: |
| 摘要 目的：比较骨髓间充质干细胞、脂肪间充质干细胞、滑膜间充质干细胞3种间充质干细胞的成软骨分化潜能，为软骨组织工程中种子细胞的选择提供实验依据。方法：采用贴壁法分别分离提取兔骨髓间充质干细胞、脂肪间充质干细胞、滑膜间充质干细胞3种间充质干细胞，并进行传代培养，绘制3种间充质干细胞的生长曲线并比较其倍增时间。将3种间充质干细胞成软骨诱导14 d后，行甲苯胺蓝染色及II型胶原免疫组化染色以观测3种细胞成软骨分化能力。结果：脂肪间充质干细胞的倍增时间短于骨髓间充质干细胞，滑膜间充质干细胞的倍增时间最短；3种细胞成软骨诱导14 d后均产生糖胺聚糖和II型胶原，且组与组之间II型胶原表达水平的差异有统计学意义，骨髓间充质干细胞组高于脂肪间充质干细胞组（P＜0.01），滑膜间充质干细胞组高于骨髓间充质干细胞组（P＜0.01）。结论：在一定的培养条件下，3种间充质干细胞均有一定的成软骨细胞分化潜能，滑膜间充质干细胞最快的增殖速度及最强的成软骨分化潜能。 |
| 英文摘要: |
| ABSTRACT Objective: To compare the chondrogenic differentiation potential of bone marrow mesenchymal stem cells(BMSCs), adipose-derived mesenchymal stem cells(AMSCs) and synovi-al mesenchymal stem cells(SMSCs). Methods: The rabbit BMSCs, AMSCs and SMSCs were respectively separated with the adherence screening method, and were continually subcultured. The growth curves of the passage 3 BMSCs, AMSCs and SMSCs were drawn, and the doubling times of three kinds of cells were compared. Then the passage 3 BMSCs, AMSCs and SMSCs were treated with chondrogenic induction. After induced for 14 days, the BMSCs, AMSCs and SMSCs were treated with toluidine blue staining and type II immunohistochemistry staining respectively to evaluate the ability of the chondrogenic differentiation potential of them. Results: The doubling time of AMSCs was shorter than that of the BMSCs. The doubling time of SMSCs was the shortest of the three. After induced for 14 days, all of them could express glycosaminoglycans and type II collagen, and there was significant difference in the expression of collagen type II between any two groups. The expression level of type II collagen in SMSCs after chondrogenic induction was higher than that in the BMSCs（P＜0.01）, and the expression level of type II collagen in BMSCs after chondrogenic induction was higher than that in the AMSCs（P＜0.01）. Conclusion: BMSCs, AMSCs and SMSCs all could transform into chondrocytes under certain conditions, but SMSCs seemed to be the fastest proliferation andthe most potential of the three. |
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