| 王 珑,贾朋丽,田旭升,李艳秋,于婷婷,申 颖,王作山,孙忠人,邹 伟.基于Wnt/β-catenin信号转导通路的电针抗抑郁中枢效应机制研究[J].,2019,19(6):1011-1015 |
| 基于Wnt/β-catenin信号转导通路的电针抗抑郁中枢效应机制研究 |
| Mechanism of Electroacupuncture Anti-depression Central Effect Based on Wnt/β-catenin Signal Transduction Pathway |
| 投稿时间：2018-10-18 修订日期：2018-11-13 |
| DOI：10.13241/j.cnki.pmb.2019.06.003 |
| 中文关键词: 抑郁 Wnt/β-catenin信号转导通路 电针 行为学 蛋白表达水平 |
| 英文关键词: Depression Wnt/β-catenin signal pathway Electroacupuncture Behavior Protein expression level |
| 基金项目:国家自然科学基金项目(81303044)；黑龙江省自然科学基金项目(H2015036)；中国博士后科学基金项目(2013M531080，2016T90320)；哈尔滨市科技创新人才专项(2014RFQXJ134)；黑龙江中医药大学创新人才支持计划项目资助；黑龙江中医药大学研究生创新科研项目（yjscx2017033，2018yjscx022） |
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| 中文摘要: |
| 摘要 目的：基于Wnt/β-catenin信号转导通路探究电针抗抑郁中枢效应机制。方法：运用随机数字表法将144只SD大鼠分为空白、模型、电针及西药组，每组又分别依据干预时间进一步分为7d、14d、21d 3个亚组。空白组不接受任何刺激。模型组大鼠采取禁食等方式构建CUMS抑郁大鼠模型。电针组选取百会、神庭穴施以电针。西药组每日灌胃给予氟西汀。分别于第1d、7d、14d、21d观测大鼠行为学指标改变情况。采用蛋白免疫印迹法检测大鼠海马内Wnt/β-catenin信号转导通路中关键蛋白表达。结果：模型组大鼠OFT、SPT评分均较空白组有所下降，体质量减轻，电针组和西药组OFT、SPT评分及体质量检测则较模型组明显上升（P＜0.05）。模型组各亚组大鼠wnt1及β-catenin蛋白表达水平下降，GSK-3β蛋白表达水平有所上升，与模型组相比，电针及西药组大鼠Wnt/β-catenin通路中蛋白含量趋近正常水平，且两组与模型组组间比较差异具有统计学意义(P＜0.05)。结论：电针可通过调控海马内Wnt/β-catenin信号转导通路中关键蛋白的表达，减轻抑郁症状。 |
| 英文摘要: |
| ABSTRACT Objective: Probe into the central effect mechanism of electroacupuncture on anti-depression based on Wnt/ esf-catenin signal transduction pathway. Methods: 144 SD rats were divided into blank group, model group, electroacupuncture group and western medicine group by random number table method. The blank group did not receive any stimulation. The model group was established by fasting and other methods. The electroacupuncture group selects baihui and shenting cave to perform electroacupuncture. In the western medicine group, fluoxetine was administered daily by gavage. Changes in behavioral indicators were observed on 1d, 7d, 14d and 21d, respectively. The key protein expressions in the Wnt/ hygro-catenin signal transduction pathway in the hippocampus of rats were detected by using protein western blotting. Results: The OFT, SPT scores and body weight of model group were obviously lower than those of the control group, while the OFT, SPT scores and body weight of the electroacupuncture group and the western medicine group were significantly higher than those of the model group(P<0.05). The expression levels of wnt1 and hygrin-catenin in rats in each subgroup were decreased, and the expression levels of gsk-3 human protein were increased. Compared with the model group, the protein levels in Wnt and hygr-catenin pathways in rats in the electroacupuncture and western medicine group were close to normal, and the comparison between the two groups and the model group was statistically significant(P<0.05). Conclusion: Electroacupuncture can reduce depressive symptoms by regulating the expression of key proteins in the Wnt/ human-catenin signal transduction pathway in the hippocampus. |
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