| 马瑞金,韩 波,杨 宇,林 珊,李 瑛.探讨As2O3诱导人胃癌SGC-7901细胞凋亡过程中AKT相关
抗凋亡通路表达情况[J].,2019,19(5):816-819 |
| 探讨As2O3诱导人胃癌SGC-7901细胞凋亡过程中AKT相关
抗凋亡通路表达情况 |
| Explore the AKT Related Anti-apoptotic Pathways Expression in Arsenic Trioxide Induced Apoptosis of Human Gastric Cancer SGC-7901 Cells |
| 投稿时间：2018-05-28 修订日期：2018-06-23 |
| DOI：10.13241/j.cnki.pmb.2019.05.004 |
| 中文关键词: 三氧化二砷 胃肿瘤 细胞凋亡 蛋白激酶B |
| 英文关键词: Arsenic trioxide Stomach neoplasms Apoptosis AKT |
| 基金项目:黑龙江省自然科学基金面上项目（H2016041） |
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| 中文摘要: |
| 摘要 目的：探讨三氧化二砷诱导胃癌细胞凋亡过程中，对AKT相关抗凋亡通路表达的影响。方法：分别用不同浓度（0 μmol/L、7.5 μmol/L、10 μmol/L、12.5 μmol/L和15 μmol/L）三氧化二砷（As2O3）溶液处理胃癌细胞48 h，倒置显微镜观察细胞凋亡情况，并用Western blot方法检测p70S6kα、p-p70S6kα、p70S6kβ（S6蛋白激酶β， Ribosomal Protein S6 Kinase β）、p-p70S6kβ、rpS6、p-rpS6、p-BAD、BAD、p-GSK3β、GSK3β及NF-κB2等蛋白的表达情况与As2O3作用浓度之间的关系。结果：随As2O3作用细胞的浓度增大，p70S6kα、p-p70S6kα、p70s6kβ、p-p70S6kβ、p-BAD、p-GSK3β、NF-κB2蛋白的表达减少，rpS6、p-rpS6、GSK3β蛋白表达增多，各组间数据经统计学分析，P值均<0.05，具有统计学意义。BAD蛋白的表达无明显改变，P>0.05。结论：As2O3诱导人胃癌SGC-7901细胞凋亡机制中包括AKT相关的多个抗凋亡途径的激活，AKT在其中发挥重要作用。 |
| 英文摘要: |
| ABSTRACT Objective: Explore the AKT related anti-apoptotic pathways expression in Arsenic trioxide induced apoptosis of human gastric cancer SGC-7901 cells. Methods: The gastric cancer cells were treated with different concentration (0 μmol/L, 7.5 μmol/L, 10 μmol/L, 12.5 μmol/L and 15 μmol/L) of As2O3 for 48 h. Cell apoptosis was observed by inverted microscope, the relationship between the expression of p70S6kα, p-p70S6kα, p70S6kβ, p-p70S6kβ, rpS6, p-rps6, p-BAD, BAD, p-GSK3β, GSK3β, NF-κB2 and the concentration of As2O3 was detected by Western blot. Results: Using Western blot assay to detect protein level, we founded that the expression of p70S6kα, p70S6Kβ, p-p70S6kα, p-p70S6kβ, p-BAD, p-GSK3β, NF-κB2 decreased, the expression of rpS6, p-rpS6, GSK3βexpression increased, Statistical analysis of each group of data showed that the P value was <0.05, with statistical significance. The expression of BAD protein had no obvious change with the concentration of As2O3 cells increased, the P value was >0.05, with no statistical significance. Conclusion: As2O3 induces the activation of multiple anti-apoptotic pathways related to AKT in the apoptosis mechanism of human gastric cancer SGC-7901, and AKT plays an important role in it. |
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