| 李海燕,贺红艳,黄凤霞,黄君华,张逍港,姚子淳.乙肝病毒X蛋白通过上调NOX4的表达活化肝星状细胞[J].,2019,19(2):227-230 |
| 乙肝病毒X蛋白通过上调NOX4的表达活化肝星状细胞 |
| HBx Protein Activated Hepatic Stellate Cells Through Up-regulationof NOX4 Expression |
| 投稿时间：2018-05-27 修订日期：2018-07-09 |
| DOI：10.13241/j.cnki.pmb.2019.02.006 |
| 中文关键词: 乙肝病毒X蛋白 NOX4 肝星状细胞 活化 |
| 英文关键词: HBx NOX4 Hepatic stellate cells Activation |
| 基金项目:国家自然科学基金青年基金项目（81700546）；陕西省教育厅西安医学院专项科研计划项目（16JK1664）；西安医学院2016年博士科研启动基金项目（2016DOC15）；西安医学院2017年配套基金项目（2017PT13） |
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| 中文摘要: |
| 摘要 目的：探讨乙肝病毒X蛋白(HBx)对肝星状细胞(HSC)活化的影响及其可能的分子机制。方法：构建稳定转染HBx基因的L02肝细胞(L02-HBx)，Western Blot鉴定细胞株中HBx蛋白的稳定表达。以L02-HBx、转染空质粒(L02-pcDNA3.1)和未转染肝细胞(L02)的条件培养基，分别孵育肝星状细胞株LX-2，即LX-2/L02-HBx、LX-2/L02-pcDNA3.1、LX-2/L02，Western Blot检测上述各组细胞α-SMA(肝星状细胞活化标志)和NOX4的蛋白表达。以L02-HBx的条件培养基孵育LX-2细胞，分为转染si-NOX4(LX-2/L02-HBx+ si-NOX4)、无关干扰片段(LX-2/L02-HBx+snc-RNA)、未转染(LX-2/L02-HBx)3组，Western Blot检测各组LX-2细胞的α-SMA蛋白表达。结果：L02-HBx中稳定表达HBx蛋白；LX-2/L02-HBx细胞中α-SMA和NOX4的蛋白表达显著高于LX-2/L02-pcDNA3.1、LX-2/L02细胞(P＜0.01)；LX-2/L02-HBx细胞敲减NOX4后(LX-2/L02-HBx+si-NOX4)，α-SMA的蛋白表达显著低于LX-2/L02-HBx+snc-RNA和LX-2/L02-HBx细胞(P＜0.05)。结论：肝细胞中表达的HBx蛋白可以通过HSC中NOX4上调α-SMA的表达，促进HSC的活化。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the mechanism of HSC activation by HBxprotein. Methods: HBx-transfected human non-tu- mour hepatic L02 cell line, named L02-HBx was established and showed HBx expression by western blot analysis. Western blot tested the effects of HBx on NOX4 and α-SMA expression in human HSC HSC cell line(LX-2) after exposure to conditioned medium from L02-HBx , L02-pcDNA3.1, and L02 cells. After LX-2/L02-HBx cells treated with si-NOX4, the NOX4 and α-SMAexpression were tested by western blot analysis. Results: The L02-HBx cell models were successfully constructed. The expression of HBx was significantly ex- pressed. Compared with LX-2/L02-pcDNA3.1 and LX-2/L0 groups, NOX4 and α-SMAexpression were increased significantly in LX-2/L02-HBx group(P＜0.01)． α-SMA in LX-2/L02-HBx expressions were depressed, coupled with the fact that the NOX4 was si- lenced(P＜0.05). Conclusion: HBx protein activate hepatic stellate cells, and NOX4 play a vital role in the activation. |
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