| 高志琴,莫小辉,陆海空,钱伊弘,柴 喆,顾 昕,管志芳,龚伟明.男性泌尿道来源的脲原体对氟喹诺酮类药物耐药性研究*[J].,2019,19(1):116-119 |
| 男性泌尿道来源的脲原体对氟喹诺酮类药物耐药性研究* |
| A Study on Resistance to Fluoroquinolones in Ureaplasmafrom Male Urinary Trac* |
| 投稿时间：2018-03-08 修订日期：2018-03-30 |
| DOI：10.13241/j.cnki.pmb.2019.01.025 |
| 中文关键词: 脲原体 聚合酶链反应 氟喹诺酮 耐药性 |
| 英文关键词: Ureaplasma Polymerase chain reaction Fluoroquinolones Drug resistanc |
| 基金项目:上海市卫生局科研计划资助项目（20134324） |
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| 中文摘要: |
| 摘要 目的：运用酶链聚合反应(PCR)技术分析和比较不同生物群的脲原体对氟喹诺酮类药物的耐药情况。方法：以脲原体16S rRNA保守区域基因为扩增靶序列检测脲原体的不同生物群，采用PCR方法扩增拓扑异构酶gyrA和parC基因并进行测序，分析基因突变与耐药的关系。结果：脲原体生物一群对左旋氧氟沙星的耐药性高于生物二群，二者差异有统计学意义（t=2.071，P=0.044）。gyrA基因主要为112号编码蛋白D112E的变异，parC基因主要为编码蛋白S83L的变异，即83 号位丝氨酸（TCA）到亮氨酸(TTA)的变异的变异。与未突变株相比，拓扑异构酶基因突变株对环丙沙星MIC存在统计学差异（P<0.001）。结论：不同生物群的脲原体对部分氟喹诺酮类耐药存在差异，拓扑异构酶基因突变与脲原体对喹诺酮类耐药存在相关性。 |
| 英文摘要: |
| ABSTRACT Objective:To explore the association between different biovars of Ureaplasma and the resistance to fluoroquinolones. Methods: Ureaplasma from male urinary tract were detected using 16s rRNA gene by PCR to identify the biovars of Ureaplasma. The relationship between biovars of Uu and symptoms was analyzed. PCR and DNA sequencing were conducted to analyze the genes of gyrA and parC. Results:There was a significant difference in the resistance to Levofloxacin between the biovar 1 of Ureaplasma and the biovar 2 of Ureaplasma (P=0.044). Sequencing analysis revealed the mutation of D112E and S83L in gyrA and parC respectively. Compared with non-mutation strain of Ureaplasma, the mutation strain had a higher level of the resistance to Ciprofloxacin (P<0.001). Conclusion: There were differences in resistance to quinolones among Ureaplasma urealyticum in different biovars of Ureaplasma and there had a correlation between the mutation of topoisomerase gene and the resistance of Ureaplasma to quinolones. |
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